Rabbit monoclonal antibody form

ProteoGenix has overcome some challenges to be able to offer a unique rabbit monoclonal antibody production service using phage display antibody library technique.

Rabbit monoclonal antibodies are a class of reagents with very high added value for research, diagnostic and therapeutic applications.

They naturally have:

 

Higher affinity (excellent for IHC usage)

Higher specificity (higher signal to noise ratio)

Excellent for post translational modification detection

 

as well as higher epitope recognition compared to other monoclonal antibodies.

Rabbit monoclonal antibodies are mostly derived from hybridomas, which are generally not well characterized and which sometimes show high batch-to-batch variability.
The hybridoma method screening is very limited (~103), often it misses rare clones and takes a while to produce antibodies (approximately 9 months). Additionally, clones are often very unstable and antibody production from them can be troublesome. In contrast, phage display combines the superior antigen recognition of the rabbit immune system with the specificity and consistency of a monoclonal antibody generated via hybridoma development without the drawbacks of rabbit hybridomas.


Advantages of rabbit monoclonal antibody production by phage display

 

Based on their binding affinity and specificity, phage display is one of the most robust technologies used for large-scale studies and the selection of proteins, peptide and antibodies. We believe that, for the production of rabbit monoclonal antibodies, using phage display offers many advantages compared to other technologies:

  • Faster and cost effective

    We can build phage display libraries very quickly. Our technology will radically reduce the time (3 – 5 months) and expenses for your project

  • Higher screening range

    (107– 109)

  • Abundance

    Once we have created libraries, we can reproduce them promptly in phage and use them for an unlimited number of screenings

  • Enhance the specificity and stability

    with which antibodies recognize antigens in various environmental conditions

  • Higher production

    Of antibody clones at a time

  • No fusion

    No stability issues of rabbit hybridomas and availability of the antibody sequence to secure it forever

  • Animal protection

    As phage display (from naive libraries) doesn’t require any animal use

ProteoGenix rabbit monoclonal antibody production services content

 

In this case we offer two possible ways to start the project by phage display:

One method is based on a naive library (coming soon), all the genes of antibodies collected from bone marrow, blood and other samples are included in the library and are suitable for selecting antibodies that match with all kinds of antigens. If you are requesting an immunization with a higher chance of getting the best binders, we offer you the possibility of building an immune library.

Below are the timeframes for the screening of both libraries:


Starting with screening a naive library

Takes about 6-7 weeks (no immunization needed)

 


Starting with construction and screening an immune library

Takes about 15-18 + 6-7 weeks (including immunization)

 


Rabbit monoclonal antibody production steps (Immune library)

 

 

Rabbit Monoclonal Antibody Production Steps : immunization of 3-5 animals, phage display library construction, Biopanning : screening, amplification and identification, validation by ELISA

 

For more information or to get a quote for our service, don’t hesitate to contact us.