ProteoGenix has solved some issues offering a unique rabbit monoclonal antibody production service using phage display antibody library technique.
Rabbit monoclonal antibodies constitute a class of reagents with very high added value for research, diagnostic and therapeutic applications.
They have naturally:
Higher affinity (excellent for IHC usage)
Higher specificity (higher signal to noise ratio)
Excellent for post translational modification detection
as well as higher epitopes recognition compared to other monoclonal antibodies. READ MORE >
Rabbit monoclonal antibodies are mostly derived from hybridomas, which are generally not well characterized and show sometimes high batch-to-batch variability.
The hybridoma method screening is very limited (~103), oftentimes it misses rare clones and it takes a while to produce antibodies (approximately 9 months). Additionally, clones are very often unstable and antibody production from them can be troublesome. In contrast, phage display combines the superior antigen recognition of the rabbit immune system with the specificity and consistency of a monoclonal generated via hybridoma development without all the drawbacks of rabbit hybridomas. .
Advantages of rabbit monoclonal antibody production by phage display
Phage display is one of the most robust technologies used for large scale study and selection of proteins, peptide and antibodies based on their binding affinity and specificity. We believe that using phage display offers many advantages compared to other technologies for rabbit monoclonal antibodies production:
ProteoGenix rabbit monoclonal antibody production services content
In this case we offer two possible ways to start the project by phage display:
One method is based on a naive library (coming soon), all the genes of the antibodies collected from bone marrow, blood and other samples are included in the library and it is suitable to select antibodies matching with all kinds of antigens. If you are requesting an immunization to have higher chance of getting best binders, we offer the possibility to build an immune library.
Below, the timeframes for the screening of both libraries:
Rabbit monoclonal antibody production steps (Immune library)
For more information or to get a quote for our service, don’t hesitate to contact us.