All our packages include a high antibody titer guarantee (1/64000 in ELISA). Our anti-protein package even includes WB positive results.
With all-included packages starting from 310.50€, we provide the most competitive price on the market!
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Save time and buy polyclonal antibodies directly online thanks to our online form!
Large choice of species
Choose between different species depending on the volume and format of antibodies you require!
We offer integrated polyclonal antibody production solutions from antigen design to purification and conjugation (if requested).
Get your polyclonal antibodies in 28 days thanks to our Expressway™ protocol.
Designed sequence for customer’s validation
Proteins: 3-5 weeks
Peptides: 3 weeks
Antigen sample (protein or peptide)
Fast: 28 days
Proteins: 51 days
Peptides: 70 days
Final Immune serum (if no purification)
-Purified polyclonal antibodies (serum of each animal is purified separately)
-Certificate of analysis (CoA)
Antibody conjugation and fragmentation
Sandwich ELISA development
To select the most suitable species for your polyclonal antibody production, several factors need to be taken into account:
The first choice for polyclonal antibody production given their size, ease of handling, and ability to produce high titers of high-affinity antibodies.
Sheep and Goats
Ideal hosts when larger amounts of antisera are needed.
Ideal hosts when generating antibodies against conserved mammalian proteins. Possibility to harvest antibodies using non-invasive methods.
Llama and Alpaca
Ideal when targeting cryptic antigens and when the final application requires a higher capacity for tissue penetration and higher antibody stability.
Host selection is an important step of every polyclonal antibody production process. Although rabbit hosts are the most conventional choice, there is a growing interest in producing chicken and camelid antibodies.
Polyclonal antibody production in chicken can be quite advantageous when the process needs to be scaled-up because IgY antibodies are extracted from egg yolk instead of serum. It is known that egg yolk is more challenging to purify than serum; however, it can be produced in higher quantities in comparison to mammalian polyclonal production.
In contrast, camelids are increasingly appreciated as polyclonal antibody production hosts. In addition to conventional IgG antibodies, they can produce immunoglobulins devoid of light chains – heavy chain antibodies (HAbs). These molecules have unique properties including:
We have the capacity to produce all types of antigens at our facilities including peptides, proteins, DNA, small molecules, and cells overexpressing the target antigen. We can also produce polyclonal antibodies using customer-provided antigens.
Achieving high titers of target-specific antibodies depends on the antigen’s capacity for eliciting a strong immune response. For this reason, choosing a suitable antigen for immunization remains one of the most important steps of the polyclonal antibody production process
Several antigens may be used for polyclonal antibody generation including:
Other antigens may be used for immunization such as small molecules or even whole cells (native or recombinant); however, these projects require the development and testing of custom immunization solutions.
Our standard immunization protocol starts at:
Both protocols can be extended if guaranteed antibody titers are not reached. Typically, it is better to immunize animals with a lower quantity of antigen and for longer periods, rather than using higher quantities of antigen and shorter immunization times.
Polyclonal antibodies are typically harvested by bleeding the hosts after desired antibody titers are reached. The cellular fraction and the antibody-enriched serum can be separated by centrifugation resulting in a crude polyclonal antibody solution.
Crude preparations are useful for many applications. However, for enhanced sensitivity and reduced off-target binding, these preparations should be purified. Polyclonal antibody purification can be carried out by:
Need advice for your custom polyclonal antibody production? Please feel free to contact your dedicated account manager!
To monitor the response to immunization, we recurrently take samples from hyperimmunized hosts to measure their antibody titers. Conventionally, this is done using ELISA, but alternatively, our clients may request antibody titer verification by Western Blot as well.
Polyclonal antibodies are a mixture of monoclonal antibodies that originated in different B cell clones. Consequently, these antibodies display different epitope-specificity and binding affinity. This property makes them highly sensitive to low abundance markers and highly effective at tackling complex targets.
Given their diverse nature, polyclonal antibodies are often classified according to the host species and antigen used for their generation. These antibodies are generally produced in rabbits, goats, and sheep. Rabbit polyclonal antibodies are particularly useful because the rabbit’s immune system can generate high-affinity antibodies in higher abundance than other host species.
Polyclonal antibodies are also named according to this classification, in this way, anti-mouse rabbit polyclonal antibodies are pAbs designed to bind to murine IgG and produced in a rabbit host.
Polyclonal antibodies are a mixture of monoclonal antibodies with distinct epitope-specificity and binding affinity. They are produced by immunizing animal hosts with a specific target (protein, peptide, DNA, etc.) and harvested by separation and purification of their serum after the development of a strong immune response.
Unlike monoclonal antibodies, prized for their high specificity and selectivity, polyclonal antibodies are known for their enhanced sensitivity. This property makes them invaluable tools for multiple applications including research (basic, medical, etc.), therapy, and diagnostics. They are particularly advantageous reagents to detect low abundance markers, toxins, among other substances. The detection of these rare markers is extremely useful for the design of early diagnostic tools, food monitoring applications, and for capturing rare targets and purifying them before further analysis.
From a therapeutic perspective, polyclonal antisera are invaluable to treat complex acute conditions like snakebite envenoming. Venoms cause acute reactions and are composed of a complex mixture of different proteins with key roles in pathogenesis. In this way, successful antivenom therapies need to be able to tackle several antigens and epitopes present in a single venom to block the pathogenic pathways and avoid adverse reactions.
From a technical point of view, polyclonal antibodies are cheaper to produce than their monoclonal counterparts. Plus, the timelines for polyclonal antibody production are significantly shorter than the lead time of monoclonal antibody production. In contrast, their most notable limitations are the difficulty in scaling up the process and high batch-to-batch variability. The impact of the latter can be mitigated by including proper controls (positive and negative) and standards in every assay.
Overcoming scale-up hurdles is more challenging, but recent breakthroughs have provided interesting solutions. One of the most interesting solutions is the purification of IgY antibodies present in egg yolk using ionic liquids. Due to the non-invasive methods of antibody harvesting and the cost-effective new purification methods, these molecules may soon gain ground over other polyclonal antibodies for a multitude of applications.
Polyclonal antibodies can be used by themselves or in tandem with their monoclonal counterparts in different assays. The most common application of polyclonal and monoclonal antibody pairs is the enzyme-linked immunosorbent assay (ELISA). In ELISA, monoclonal antibodies (also called primary antibodies) are typically used to bind a specific target, while polyclonal antibodies (secondary antibodies) are used to bind the primary antibodies.
In this configuration, the primary antibodies are “naked” (no tags) while the secondary antibodies carry the enzymatic tag. This format allows the amplification of the detection signal, lowering detection thresholds considerably. The signal amplification occurs because, while primary antibody binds to a single epitope on the target molecule, secondary antibodies bind to multiple regions of the primary antibodies. In this way, multiple secondary antibodies conjugated with an antibody can coat the surface of the primary antibody, dramatically enhancing the detection signal.
The enhanced sensitivity of these molecules comes with its own set of specific hurdles. For instance, polyclonal antibodies are notorious for their propensity for off-target binding, which may lead to false-positive results. To overcome this hurdle, polyclonal antibodies must be submitted to stricter purification processes (e.g. by performing antigen-specific antibody purification). Additionally, the inclusion of controls and standards can help researchers correctly identify thresholds of detection.
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