+ Custom polyclonal antibodies VS Custom monoclonal antibodies
Comparison between monoclonal and polyclonal properties for custom antibodies production
Monoclonal antibodies Polyclonal antibodies
Specificity One hybridoma = one single antibody type, detecting one single epitope present on the immunogen.

Avoids cross-reactivity issues with other target proteins.

Possible to select a clone the most efficient in the given application (protein function inhibition, flow cytometry, sandwich ELISA)
Unpurified serum:

Pool of antibodies, including non-antigen-specific antibodies, binds different epitopes of the antigen.

Can generate background and cross-reactions with other proteins.
Affinity purified serum:

Sample purity = 99.99%

Specific for the antigen targeting all accessible and immunogenic epitopes of the immunogen.

Low background and potential cross-reactions with other proteins.
Sensitivity Sometimes less sensitive because of the high specificity for only one epitope.
A good selection of the most sensitive monoclonal antibody can allow strongly binding ones to be found.
Sometimes more sensitive due to the wide variety of different antibodies represented in the serum.
Lead time ~6 months ~2-3 months (can be less with our Expressway protocol in 28 days)
Cost 3 to 4 times more expensive than the cost of custom pAbs. 3 to 4 times less expensive than the cost of custom mAb.
Reproducibility Antibodies always identical

Many lots of identical antibodies can be produced over dozens of years.

Possible to sequence the hybridoma to make recombinant antibodies.


Different antibodies from batch to batch.

Require new immunizations with other animals.
Quantities Almost unlimited quantities.

Production possible over many years, as long as clones are viable.
Maximum of a few mg / animal.
Available species Rat, mouse Rat, mouse, rabbit, chicken, guinea pig, sheep, and goat.
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As an experienced antibodies manufacturer, ProteoGenix has successfully raised more than 1500 polyclonal antibodies during the past 13 years.

+ I want to provide my own antigen, what quantity do you need ?

Step 1: Define your package

  1. Antigen from customer
    2 rabbits
    Antigen Purification

    PACK 1
  2. Anti-Peptide
    2 rabbits
    Antigen Purification

    PACK 2
  3. Gene Synthesis
    E. Coli Protein Production
    2 rabbits
    Antigen Purification
    PACK 3
  4. Antigen from customer
    2 rabbits
    No purification
    PACK 1XS
  5. Anti-2 Peptides
    2 rabbits
    Antigen Purification

    PACK 2.2
  6. Phospho-Specific
    2 rabbits

    PACK 4
  7. Design
    your own

More information regarding our custom antibodies production services:

Antibodies development

ProteoGenix has more than 14 years of experience in this field with more than 1500 antibodies generated including a large number of rabbit polyclonal antibodies.
Our expertise in biological engineering such as gene and peptide synthesis, recombinant protein production and polyclonal antibodies production services allows us to provide very complete services. Thank to our long experience on these domains (from cDNA to antibody) we can determine the best strategy for each project depending on the needs of each one of our customers.

Antibodies services

We offer a One-Stop solution for your polyclonal antibodies generation project as we are able to cover all the different steps if needed:
• Antigen production (peptide synthesis / recombinant protein production)
• Animal Immunizations – 7 species available: Rabbit, Chicken, Mouse, Rat, Guinea Pig, Sheep and Goat antiserum
• Antibodies Purification against protein A or G or against the antigen
• Antibodies Conjugation

Antibodies manufacturer

As an experienced antibody manufacturer, we have a high quality commitment and that’s the reason why we guarantee: • ELISA results : 1/32 000 for crude serum and 1/64 000 for purified antibodies (for anti-protein polyclonal antibodies services)
• The final quantity of antiserum or of purified antibodies (depends on the species and the package the amount of rabbit antiserum is higher than guinea pig antiserum but lower than goat antiserum)
• WB guarantee for the anti-protein polyclonal antibodies package n°3
We also offer a phospho-specific polyclonal antibodies package.

Experienced antibodies manufacturer:

For polyclonal antibody development, we have carried out thousands of different projects, in particular for: Pharmaceutical / diagnostic / veterinary / cropscience / food agribusiness multinationals such as Bayer, Sanofi, Servier, or Virbac.
Public research centers such as CNRS, INSERM and Institut Pasteur in France.

Animals immunization protocol

Our standard immunization protocol is 51 days – 4 injections for anti-protein polyclonal antibody (with possibility to extend the protocol if the guarantees are not reached)
For anti peptide polyclonal antibodies, our standard protocol is 70 days – 5 injections (with possibility to extend the protocol if the guarantees are not reached)

It is usually recommended to immunize animals with a lower quantity of antigen and for a longer time than to immunize the animals on a shorter timing with a larger quantity of antigen.

If this seems too long, we also propose our Xpressway protocol of 28 days (Fast polyclonal antibody production) which is made using our special adjuvant which is aimed to increase the immune response.
It is usually only recommenced for anti-protein polyclonal antibodies because peptides might sometimes be not immunogenic enough to give a good immune response on this short period of time.

Species available at ProteoGenix

About animal species, rabbit polyclonal antibodies are usually the first choice for pab generation, however other species should be considered.
When looking for larger amounts of antiserum it may be more beneficial to use polyclonal antibodies from a larger animal such as sheep polyclonal antibody or goat polyclonal antibody.
Usually we advise to select an animal which is genetically distant from the antigen source (for example chicken polyclonal antibodies are very suitable to develop polyclonal antibodies against conserved mammalian proteins).

We also recommend our customers to use at least 2 animals per project. There is often a variability of immune response between individuals, so it increases the probability to get a highly reactive and specific polyclonal antibody targeting your antigen of interest.

Feel free to complete the online form above to design you own package !