You need to get an antibody with reduced immunogenicity and high specificity and stability? Use our huge human (2×1010 different clones) and rabbit (1.12×1010 different clones) Fab libraries. In ProteoGenix, our experts make sure you receive at least 3 different binders within only 4 weeks.
We guarantee at least 3 binders to your antigen of interest
Your Fab in 4 weeks
Get your full Fab antibody sequence within maximum 4 weeks
High Fab human and rabbit naïve libraries
Maximize the number ofrelevant binders using our huge diversity human and rabbit Fab libraries
Immune Antibody Library construction
Benefit from our expertise in antibody production to construct your own immune library if you need an antibody with maximized binding affinity
No animal uses
Naïve library screening does not require any animal use
Be the owner of the Fab antibody sequence that we generate for you
Immune Library construction
Fab library screening and Biopanning
Screening and validation by ELISA
Phage DNA extraction + antibody screening
Fab sequence delivery to the customer
Recombinant antibody production
Stable cell line development
Fab fragments, with a size of 50 kDa, represent the antigen binding fragment of a full-length antibody. They contain both the variable region of heavy chain (VH), a variable region of light chain (VL), a constant region of heavy chain 1 (CH1) and a constant region of light chain (CL). The fragments which contain disulfide bridge thiols are named Fab’ fragments, whereas those lacking the thiol functional group are called Fab fragments.
There are three different methods to generate Fab fragments:
Fab antibodies are used as powerful molecular tools in therapeutic and clinical applications as well as in research, thanks to their several advantages. First, their small size allows them a fast and efficient deep tissue penetration and clearance. Moreover, lacking the Fc-mediated effector functions reduces immunogenicity and immune reactions. In addition, the monovalent format of Fab and Fab’ antibodies lowers cross-linked immunocomplexes that can trigger anaphylaxis. To date, many Fab antibodies have received, or continue receiving FDA approval for a wide range of uses, mainly therapeutic, clinical, diagnostic and research applications. Here is a non-exhaustive list of some Fab applications reported to date:
Fab and scFv antibodies have several similarities as they both are made of fragments of an IgG. These fragment antibodies have several features making them an outstanding tool in modern medicine and in scientific research fields. Although they have shared features, these two antibodies have some specific characteristics which help to select the antibody of interest depending on the use and the different purposes. This table is a brief comparison between Fab and scFv antibodies where some key characteristics are highlighted.
Got a question or need a quote? Message us and we'll get back to you 48 hours or less.