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Are you looking for a high-performing custom cat monoclonal antibody? Look no further than ProteoGenix’s cat antibody phage display service. Simply tell us what feline antigen you would like to target and we will send you three novel antibodies verified to bind your target… in fewer than 7 weeks. Each antibody is ethically sourced using animal-free methods from our prebuilt antibody libraries, ensuring maximized antibody library diversity for identifying high-affinity antibodies. With our extensive libraries and over 300+ successful phage display projects on record, we are confident we can help you obtain the perfect antibody for research, diagnostic, or therapeutic needs. Speak with one of our highly skilled antibody professionals today!
Huge Antibody Diversity
Exploring vast antibody diversity in phage display libraries derived from many Cat species in scFv and Fab antibody formats.
You possess sole ownership of each custom monoclonal antibody that we isolate.
You will receive at least three distinct antibodies that bind your specific cat antigen with precision.
Antibody Delivery in less than 7 Weeks
Rapidly receive your custom antibody solutions, tailored to your specifications, in just a matter of weeks.
Animal-free Cat Antibody Discovery
We isolate novel feline antibodies from a preexisting library of antibody candidates, an ethical source of monoclonal antibodies.
Streamline your tasks with our extra services – personalized antigen production and thorough antibody developability evaluation. We manage everything!
We have successfully delivered 300+ custom monoclonal antibodies using our antibody phage display services
Constant communication from PhD Account Managers
We update you on every project milestone allowing you to evaluate our progress so we stay on target and on budget.
Antigen procurement or design
Your cat-specific antigen can be acquired by:
Library Screening and Biopanning
ELISA Screening of Single Phage Binders
DNA Extraction & Antibody Sequencing
Recombinant Antibody Production
Therapeutic Antibody Production
Stable Cell Line Development
The use of a cat monoclonal antibody library is crucial for precision in researching, diagnosing, and treating specific feline diseases. Any antibody that is required for therapeutic use in felines will need to be isolated from a cat-specific phage display library to avoid immune reactions.
Creating a feline antibody for phage display library involves isolating whole blood samples from affected or healthy cats. Next, the nucleated cells in the blood are isolated and the VH and VL chains are reverse-transcribed into a DNA copy of the mRNA (cDNA) and amplified by PCR. The amplicons are then cloned into a phage display vector, fusing the cDNA to a cell surface phage protein (pIII or pillinIII).
The cat antigen is then used to isolate a unique antibody, using biopanning, from a diverse cat antibody repertoire. Veterinary scientists can use this library to isolate novel monoclonal antibodies for various research, diagnostic, and therapeutic needs. The benefits of a cat antibody library include:
Suitability for Feline Disease Models:
Versatility in Research:
Customization and Engineering:
Availability of Diverse Libraries:
Contribution to Comparative Medicine:
Our current phage display libraries are built from human, rabbit, dog, and camelid (camel, llama, and alpaca) species. These libraries are perfect for making custom antibodies for research or diagnostic use. However, antibodies used developed into therapeutics would require constructing a naïve cat antibody library to maintain species compatibility and to prevent cross-species immune reactions.
Conventional methods for developing cat monoclonal antibodies involve subjecting the cat to multiple rounds of immunization and extraction of antibody-producing cells from the cat’s blood. This approach is known to induce stress and discomfort in the feline subjects.
However, ProteoGenix’s antibody phage display strategy offers a more humane and efficient approach to generating cat monoclonal antibodies for the following reasons:
The utilization of cat antibody libraries offers a paradigm shift in ethical considerations, promoting responsible and humane practices in antibody development while advancing the field through innovative and animal-friendly approaches.
Cat monoclonal antibody libraries can be used by veterinary scientists or physicians who:
Below are specific applications cat monoclonal antibody libraries can be used for:
These are just a few examples demonstrating the power of cat monoclonal antibody libraries. Book a free call with one of our antibody experts today and learn how affordably we can make your custom cat monoclonal antibody library.
Cat monoclonal antibody libraries can be used to identify and isolate antibodies that selectively bind human antigens with precision. The resulting antibodies can be used for research purposes or clinically. However, it’s important to note that the in vivo use of a cat monoclonal antibody in a human would require antibody humanization to avoid unwanted immune reactions.
The antibody phage display technique is a laboratory approach crafted to explore interactions between antibodies and diverse molecules, including antigens. This involves genetically modifying bacteriophages, viruses infecting bacteria, by merging antibody genes with a coat protein gene of the phage. Through this genetic alteration, the phage exhibits the antibody on its surface while maintaining the antibody gene within the bacteriophage.
This genetic link between genotype and phenotype allows for the identification of antibodies interacting with specific antigens via screening the displayed phages against them. Mimicking natural selection and referred to as in vitro selection, this process facilitates thorough screening and amplification of antibody libraries.
The initial phase of constructing a cat antibody library for phage display involves integrating antibody variable region genes into a phage display vector. This begins by extracting mRNA from B-cells obtained from an animal or human patient, followed by converting mRNA into cDNA through reverse transcription. PCR amplification, using antibody cDNA as a template, generates variable regions for both heavy and light chains of each antibody.
Post-amplification, these variable regions are inserted into a modified phage display vector expressing them as a fusion protein with a coat protein on the phage’s surface. This structure allows visible presentation of antibodies on the phage’s exterior, with the DNA encoding the antibody enclosed within the phage particle.
Subsequently, the library undergoes screening against a specific target antigen to identify phages displaying antibodies capable of binding to the target. Successful phages are isolated, allowing for elution, and subsequent DNA sequencing of the antibody-encoding DNA facilitates the cat monoclonal antibody production for further examination or therapeutic applications.
A naive cat monoclonal antibody library for phage display incorporates genes from an organism unexposed to the specific antigen, while an immune library integrates genes from an organism immunized with the antigen. Naive libraries offer diverse antibodies without antigen-driven selection, increasing the potential for unique binding properties.
In contrast, immune libraries contain antibodies chosen for their affinity to the specific antigen, valuable for rapid identification. The primary distinction lies in gene origin: naive libraries encompass a broad spectrum of antibodies unfamiliar with the antigen, whereas immune libraries comprise antibodies selected for their capability to bind to that specific antigen.
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