You want to develop a monoclonal antibody conserving its native properties with maximized developability? With our single B-cell screening service, get a highly affine antibody preserving a natural VH and VL pairing and a maximum B-cell diversity. We guarantee you receive minimum 3 antibody sequences within maximum 2 months from animal immunization until antibody screening thanks to our high throughput screening platform.
Why choose ProteoGenix for your Single B-cell
screening service?
3 binders guaranteed
We guarantee at least 3
binders to your antigen of interest
Maximized developability
We make sure you get antibodies with best
physicochemical characteristics to get
maximum developability
High throughput screening
Our screening method allows to
perform different screening in a
highly efficient manner
Native monoclonal antibody
We guarantee a VL and VH natural
pairing
Maximum diversity
Our screening method generates a high
repertoire of antibodies thus preserving B
cell natural diversity
Pay only if satisfied
If you get 3 binders, pay 100%
If no binders, we charge minimal fees only
Market’s leading Antibody solution
Benefit from our system optimized for
high yields and high-throughput
antibody production
Your antibody in 2 months
Get your antibody sequence within
2 months for the whole process including
animal immunization
IP free
Be the owner of the antibody
sequences that we generate for you
Workflow of Single B Cell Antibody screening
IMMUNIZATION
- Antigen checking by SDS-PAGE
(Antigen provided by You or designed by us) - Rabbit immunization
4-6 rounds of injections using an optimized protocolImmunization is performed on Rabbits
6-8 weeks
SINGLE B-CELL
SORTING & SCREENING
- Isolation of PBMCs and spleen lymphocytes
- Fluorescence-Activated B-Cell Sorting (FACS) antigen-specific single B-cells sorted (1cell/well)
- B-cell culture and supernatant screening against antigen in ELISA
2-3 weeks
Want to test additional clones?
RECOMBINANT RABBIT ANTIBODY
(IgG) PRODUCTION & SCREENING
- Best positive clones sequencing
- Transient expression into high performance proprietary XtenCHO™
- B-cell culture and supernatant screening against antigen in ELISA
Want additional ELISA?
ELISA for positive/negative screening
(Against one specific peptide/protein/small
molecule- cross reactivity profiling)
Competitive ELISA
(To identify clones with blocking/
neutralizing activity)
Advantages of Single B cell sequencing
Single B-cell sequencing is an efficient monoclonal antibody development and screening strategy which principle is based on the amplification of genes encoding the VH and VL region from B-cells directly. This technology presents several advantages making it a very useful approach for antibody biology understanding and for antibody use in clinical essays. Among these advantages, single B-cell sorting is characterized by:
- High throughput B-cell sorting – Single B-cell sequencing integrates high throughput platforms for antibody selection which allows to perform different screenings in a highly efficient manner
- Native monoclonal antibody preservation – The main advantage of single B-cell sorting technology is that it preserves the native VH and VL pairing, two domains of monoclonal antibodies critical for antigen recognition and binding. Thus, natural cognate pairing is maintained and development of antibodies with high affinity, stability and specificity is guaranteed.
- Rare antibodies identification – Some antibodies with relevant therapeutic properties are hard to identify. Thanks to the single B-cell screening approach, some rare antibodies are identified directly from B-cells and this limits the risks of unnatural pairing resulting from in vitro screening. Moreover, this approach is ideal for discovery of rare antibodies against challenging targets such as conformational epitopes.
- Antibody diversity maximization – Thanks to the highly sophisticated screening method, single-B cells are screened individually leading to a various repertoire of monoclonal antibodies which increases the B cell diversity.
- Higher efficiency and expedited timelines – Single B-cell screening is a very efficient technology characterized by an unprecedented single-cell-resolution and an exceptional rapid timeline saving months of work as a result of avoiding the cell fusion (hybridoma) and library construction (phage display) steps.
Single-B cell vs other monoclonal
antibody screening methods
To date, several techniques have been developed to isolate and screen monoclonal antibodies (mAbs). This table summarizes the main advantages and drawbacks of each technique.
Pro | Cons | |
---|---|---|
Hybridoma |
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Phage Display |
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Single B-Cell Sorting |
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