Single B Cell Screening Service

Protein expression form

    You want to develop a monoclonal antibody conserving its native properties with maximized developability? With our single B-cell screening service, get a highly affine antibody preserving a natural VH and VL pairing and a maximum B-cell diversity. We guarantee you receive minimum 3 antibody sequences within maximum 2 months from animal immunization until antibody screening thanks to our high throughput screening platform.

    Why choose ProteoGenix for your Single B-cell
    screening service?

    3 binders guaranteed by single B cell screening
    3 binders guaranteed

    We guarantee at least 3
    binders to your antigen of interest

    Maximized developability with single B cell screening service
    Maximized developability

    We make sure you get antibodies with best
    physicochemical characteristics to get
    maximum developability

    High Throughput screening
    High throughput screening

    Our screening method allows to
    perform different screening in a
    highly efficient manner

    Native monoclonal antibody by single B cell Screening
    Native monoclonal antibody

    We guarantee a VL and VH natural
    pairing

    Maximized antibody diversity with single B cell screening
    Maximum diversity

    Our screening method generates a high
    repertoire of antibodies thus preserving B
    cell natural diversity

    Pay our single B cell screening service only if satisfied
    Pay only if satisfied

    If you get 3 binders, pay 100%
    If no binders, we charge minimal fees only

    XtenCHO Market’s leading antibody solution
    Market’s leading Antibody solution

    Benefit from our system optimized for
    high yields and high-throughput
    antibody production

    Your binders in 2 months
    Your antibody in 2 months

    Get your antibody sequence within
    2 months for the whole process including
    animal immunization

    IP free single B cell screening service
    IP free

    Be the owner of the antibody
    sequences that we generate for you

    Workflow of Single B Cell Antibody screening

    IMMUNIZATION
    • Antigen checking by SDS-PAGE
      (Antigen provided by You or designed by us)
    • Rabbit immunization
      4-6 rounds of injections using an optimized protocolImmunization is performed on Rabbits

    6-8 weeks

    SINGLE B-CELL
    SORTING & SCREENING
    • Isolation of PBMCs and spleen lymphocytes
    • Fluorescence-Activated B-Cell Sorting (FACS) antigen-specific single B-cells sorted (1cell/well)
    • B-cell culture and supernatant screening against antigen in ELISA

    2-3 weeks

    RECOMBINANT RABBIT ANTIBODY
    (IgG) PRODUCTION & SCREENING
    • Best positive clones sequencing
    • Transient expression into high performance proprietary XtenCHO™
    • B-cell culture and supernatant screening against antigen in ELISA

    2 weeks

    Want to test additional clones?
    Want additional ELISA?

    Additional services

    ELISA for positive/negative screening
    (Against one specific peptide/protein/small
    molecule- cross reactivity profiling)

    Competitive ELISA
    (To identify clones with blocking/
    neutralizing activity)

    Advantages of Single B cell sequencing

    Single B-cell sequencing is an efficient monoclonal antibody development and screening strategy which principle is based on the amplification of genes encoding the VH and VL region from B-cells directly. This technology presents several advantages making it a very useful approach for antibody biology understanding and for antibody use in clinical essays. Among these advantages, single B-cell sorting is characterized by:

    • High throughput B-cell sorting – Single B-cell sequencing integrates high throughput platforms for antibody selection which allows to perform different screenings in a highly efficient manner
    • Native monoclonal antibody preservation – The main advantage of single B-cell sorting technology is that it preserves the native VH and VL pairing, two domains of monoclonal antibodies critical for antigen recognition and binding. Thus, natural cognate pairing is maintained and development of antibodies with high affinity, stability and specificity is guaranteed.
    • Rare antibodies identification – Some antibodies with relevant therapeutic properties are hard to identify. Thanks to the single B-cell screening approach, some rare antibodies are identified directly from B-cells and this limits the risks of unnatural pairing resulting from in vitro screening. Moreover, this approach is ideal for discovery of rare antibodies against challenging targets such as conformational epitopes.
    • Antibody diversity maximization – Thanks to the highly sophisticated screening method, single-B cells are screened individually leading to a various repertoire of monoclonal antibodies which increases the B cell diversity.
    • Higher efficiency and expedited timelines – Single B-cell screening is a very efficient technology characterized by an unprecedented single-cell-resolution and an exceptional rapid timeline saving months of work as a result of avoiding the cell fusion (hybridoma) and library construction (phage display) steps.

    Single-B cell vs other monoclonal
    antibody screening methods

    To date, several techniques have been developed to isolate and screen monoclonal antibodies (mAbs). This table summarizes the main advantages and drawbacks of each technique.

    Pro Cons
    Hybridoma
    • High antibody affinity
    • Preservation of natural VH-VL pairing (positively impacts developability)
    • Cheap
    • High loss of diversity due to low efficiency of cell fusion
    • Limited to rodents as myeloma cells not efficient in other species
    • Humanization needed
    • Not adapted to antigens with low immunogenicity and/or toxicity
    • Animal use
    Phage Display
    • No species restriction
    • No need for humanization thanks to fully human libraries & humanized mice
    • Maximized diversity due to random VH-VL pairing
    • High-throughput screening allowing identification of multiple binders
    • Adapted to antigens with low immunogenicity and/or toxicity
    • Very fast
    • No animal use thanks to premade libraries
    • Non-natural VH-VL pairing can negatively impact developability
    • Potential bias due to phage particle
    • Low/medium antibody affinity due to lack of immunizations (naïve libraries)
    • High affinity antibodies expensive & long to develop as require custom immune library construction
    Single B-Cell Sorting
    • No species restriction
    • High antibody affinity
    • Preservation of natural VH-VL pairing
    • High-throughput screening
    • Best for discovery of rare antibodies against challenging targets (e.g. conformational epitopes) with maximized developability
    • Not adapted to antigens with low immunogenicity and/or toxicit
    • Animal use