As a leading supplier of products and services in recombinant proteins, ProteoGenix offers expression optimization services for difficult or large scale recombinant protein productions through High Throughput Screening (HTS) of expression conditions.

checkCost effective solution to accelerate your project development

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Up to 1000 conditions can be tested

check2 – 3 weeks

checkLow cost per test

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Advantages of using high throughput screening

 

It is widely accepted that for some difficult to express proteins in heterologous systems, it is essential to test a large number of conditions at every steps of the process to identify the optimal expression protocol to:

 

  • Reach the highest possible yield

  • Produce in soluble form

  • Preserve protein activity

  • Reach the highest purity

  • Define the most stable formulation

High throughput expression condition screening example steps

 

 

ProteoGenix offers an example of a one-stop solution from the gene to production via bioreactor or fermentor. The "HTP" screening can intervene after the production of proteins in order to obtain a stable expression and optimize your project as much as possible.

ProteoGenix High Throughput screening services content

Thanks to state-of-the-art scientific know-how, ProteoGenix is capable of screening more than a thousand conditions in a few days. We usually work out the following conditions:

  • Construction (Vector, SIP, purification tag, solubility tag…)
  • Strain
  • Culture condition
  • Medium composition
  • Extraction condition
  • Purification
  • Lyophilization

With our high quality automation systems, get the opportunity to identify hit compounds for your reasearch projets or for drug discovery.

Major equipments:

  • BC workstation system for culture
  • Qpix for colony picking
  • Octet Red96
  • Akta (Avant, Explorer, Pilot and Purifier)

For more information or to get a quote for our high throughput screening services, don’t hesitate to contact us.

Related sections which could interest you:

  • Protein Production in coli
  • Protein Production in subtilis
  • Protein Production in insect cells / baculovirus
  • Protein Production in mammalian cells
  • Protein Production in yeast
  • Recombinant monoclonal antibody production
  • Stable cell line development