Our high throughput protein expression test service is the solution to overcome the most demanding challenges regarding difficult-to-express proteins! ProteoGenix developed a HTP platform dedicated to protein expression optimization. Our solution: 1000+ expression conditions carefully selected and tested in parallel to skyrocket your protein production in a short timeline.
Protein expression “à la carte”
We design the most suitable expression conditions to test to meet the specifications.
We can perform 1000 tests in 4 weeks. Thanks to our fully automated high throughput platform.
The ULTIMATE solution for difficult-to-express proteins
A challenging protein expression? Our high throughput protein expression screening overcomes any challenge.
Unique protein expression expertise
We expressed over 1500 proteins since 2003.
5 expression systems
We have a deep understanding of 5 different expression systems: E. coli, B. subtilis, yeast, baculovirus/insect cells, and mammalian cells.
Our service can include our high-performance mammalian expression system.
ProteoGenix combines the knowledge of its best protein production experts to provide you the most advanced high throughput protein expression platform. Our high throughput platform was designed following one rule: “The more relevant conditions we test, the more probability we have to find the way to express your protein successfully”. Thus, our high throughput protein expression platform allows us to test 1000 conditions in 4 weeks in order to meet or even outperform your requirements in terms of:
Achieving such results is only possible by proposing a wide range of protein expression conditions. Here is a list of parameters ProteoGenix can vary:
Are you facing difficulties in protein expression optimization? Contact our PhD account managers to unlock the potential of your production.
Determination of best host/vector pair
Optimization of protein expression in flask
Optimization of protein purification
2 Best System Contructions Combinations
Best Expression Condition Selection
200 media screening
5 best fermentation conditions
Production of difficult-to-express proteins requires varying several parameters that can strongly influence the final yield, purity, stability, solubility and biological activity. If we take a closer look at the productivity more specifically, here are some of the parameters to consider for protein expression optimization:
The choice of the best expression system is of primary importance when it comes to protein expression. This choice will depend on your requirements: high productivity, proper protein folding, “human-like” post-translational modifications or a compromise between all of these requirements… Here is a small table explaining the essential parameters to consider when choosing the right expression system:
Gene synthesis should be optimized for the host organism by the so-called codon optimization process. This optimization aims at avoiding rare codons in the mRNA sequence thus limiting the risk of protein expression failing and increasing the chance to get a high yield protein production.
Protein expression optimization is also partly driven by the choice of the best expression vector. The non-coding sequence part regulates several elements essential to protein expression:
Several factors are known to influence protein expression:
This list is non-exhaustive and represents only part of the parameters ProteoGenix can optimize thanks to its high throughput protein expression service. Contact us to talk about your specific requirements with our PhD account manager who will define with you the best parameters to vary to optimize your protein expression.
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