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ANTIBODY SEQUENCING SERVICES

HomeTherapeutic antibody development / Antibody sequencing

Entrust experienced professionals for antibody sequencing services.
Getting your monoclonal antibody sequence is a key starting point :

Hybridoma sequencing scientist
  • to protect you antibody sequence by patent application
  • to optimize the potential of your antibody by further engineering
  • to prevent the loss of the hybridoma cell line
  • for recombinant antibody production.

Why choose ProteoGenix for your
antibody sequencing?

Hybridoma sequencing competitive price

Competitive price

Our prices are among the most competitive on the market!

Guaranteed DNA sequencing service

No sequence – no fee

We do not succeed in determining the sequence? You don’t pay!

Fast antibody sequencing service

Fast turnaround

Benefit from a fast service thanks to our standardized process!

Antibody engineering experts

Monoclonal antibody development expert

Increase your success rate by using our engineering and production services!

Custom antibody sequencing process

Custom process

Get an antibody sequencing service strictly adapted to your requirements!

One stop solution from sequencing to therapeutic antibody

One stop solution

From early antibody discovery to the clinic!

Our antibody sequencing process:
a one-stop solution from your hybridoma cell
line to your antibody sequence

Hybridoma cell line
Hybridoma cell line delivery
Hybridoma mRNA extraction
mRNA extraction
Reverse transcription for protein sequencing

Reverse transcription

DNA sequencing
DNA sequencing of at least 5 samples for sequence alignment
Antibody sequence subcloning
Sub-cloning into a standard vector
PCR amplification before antibody sequencing
PCR amplification
PCR amplification before antibody sequencing
PCR amplification
Antibody sequence subcloning
Sub-cloning into a standard vector
DNA sequencing
DNA sequencing of at least 5 samples for sequence alignment
Antibody sequence analysis
Data analysis and report writing
Antibody-antigen interaction
Optional: test expression + antibody/antigen interaction test

ProteoGenix proposes a fully customizable antibody sequencing service with high guarantees. After 16 years of experience, our services are exhaustive and standardized and featured as follow for optimal performances:

  • Length of the sequence: antibody variable domains (VH, VL), full-length antibody (VH, VL, Fc)
  • Optional:
  • Deliverables:
    • A complete report including the CDR sequence annotated,
    • Plasmid containing the sequence (if option chosen),
    • Up to 2mg recombinant antibody (if option chosen).

Case study: DNA sequencing of variable
regions of hybridoma cell line

Aim of the study

Here is an example of a customer requesting us to sequence a hybridoma cell line we previously developed for him.

Materials

  • PacBio RS II
  • Hybridoma cells provided by ProteoGenix
  • TaKaRa MiniBEST Universal RNA Extraction Kit
  • PrimeScript RT reagent Kit with gDNA Eraser
  • TA-cloning Kit

Hybridoma sequencing protocol

Total RNA was extracted from hybridoma cells and cDNA subsequently synthesized. Antibody variable genes were then amplified by isotype-specific PCR, sub-cloned into a standard cloning vector separately and sequenced.

Isotype determination and antibody sequence analysis

Antibody isotype determination is performed by gel electrophoresis. Details of the protocol are described in the complete report provided as PDF file.

Results of the sequencing are based on the analysis of at least 10 positive clones and include:

  • Gene sequence
  • Amino acid sequence with annotated CDR

After completion of the antibody sequencing work, we were requested engineer a chimeric version, synthesize both antibody cDNAs, subclone them into an expression vector, produce them in XtenCHO cells and determine antibody binding affinity (KD determination) of all 3 antibodies. The purpose was to confirm that the chimeric recombinant antibody functionality remained at least equivalent to the mouse recombinant antibody and the mouse hybridoma antibody.

After confirmation, we were then able to develop humanized antibodies as well as bispecific antibodies for human therapeutic purposes.

Click on the button below to get the complete report “Parental Antibody Sequencing from Hybridoma”

Why should you sequence your antibody?

Patent your antibody sequence
  • Patent application

The development of a monoclonal antibody can represent years of research. Demonstrating the uniqueness of your sequence is the first step to patent it and to secure your past and future investments.

Antibody engineering
  • Antibody engineering

Antibody sequencing provides valuable data for further engineering modifications. For example, antibody humanization or affinity maturation can be performed once your mAb sequence has been obtained. Get also access to various antibody formats such as bispecific antibodies or antibody-drug conjugates.

Recombinant antibody production
  • Recombinant production

Therapeutic antibody development requires a stable cell line able to produce a high amount of stable antibodies without contaminants. Accessing to your antibody sequence allows you to benefit from the advantages of recombinant antibody production: high reproducibility, ease of scalability and animal free production

Hybridoma sequence safety
  • Safety

Hybridoma cell lines can be lost due to gene loss, gene mutation or contamination. Your hybridoma cell line sequence is the guarantee that you can continue recombinant antibody production even if your hybridoma line is lost.

What comes after your antibody
sequence analysis?

With more than 15 years of experience in antibody development and production, ProteoGenix can offer you far more than your hybridoma sequence. Using our antibody sequencing service can be the starting point of a long and fruitful collaboration that will significantly increase your chance to go to the clinic. ProteoGenix proposes a one-stop solution tailored to bring your project further such as:

Therapeutic antibody development

Therapeutic antibody
development

ProteoGenix offers high-quality engineering solutions to optimize the properties of your therapeutic antibodies including antibody humanization, affinity maturation, bispecific antibodies, antibody drug conjugates…

Guaranteed recombinant antibody expression

Guaranteed recombinant
antibody production

ProteoGenix provides one of the most performing recombinant antibody production platform in the world thanks to 16 years of experience and proprietary technologies like XtenCHOTM.