Antibody sequencing service

Antibody sequencing form

    Entrust experienced professionals for antibody sequencing. Getting your monoclonal antibody sequence is a key starting point :

    Hybridoma sequencing scientist
    • to protect you antibody sequence by patent application
    • to optimize the potential of your antibody by further engineering
    • to prevent the loss of the hybridoma cell line
    • for recombinant antibody production.

    Why choose ProteoGenix for your antibody sequencing?

    Hybridoma sequencing competitive price

    Competitive price

    Our prices are among the most competitive on the market!

    Guaranteed DNA sequencing service

    No sequence no fee

    If we do not succeed in determining the sequence? You don’t pay!

    Fast antibody sequencing service

    Fast turnaround

    Benefit from a fast service thanks to our standardized process!

    Antibody engineering experts

    Monoclonal antibody development expert

    Increase your success rate by using our engineering and production services!

    Custom antibody sequencing process

    Custom process

    Get an antibody sequencing service strictly adapted to your requirements!

    One stop solution from sequencing to therapeutic antibody

    One stop solution

    From early antibody discovery to the clinic!

    Our antibody sequencing process:
    a one-stop solution from your hybridoma cell
    line to your antibody sequence

    Hybridoma cell line
    Hybridoma cell line delivery
    Hybridoma mRNA extraction
    mRNA extraction
    Reverse transcription for protein sequencing

    Reverse transcription

    DNA sequencing
    DNA sequencing of at least 5 samples for sequence alignment
    Antibody sequence subcloning
    Sub-cloning into a standard vector
    PCR amplification before antibody sequencing
    PCR amplification
    PCR amplification before antibody sequencing
    PCR amplification
    Antibody sequence subcloning
    Sub-cloning into a standard vector
    DNA sequencing
    DNA sequencing of at least 5 samples for sequence alignment
    Antibody sequence analysis
    Data analysis and report writing
    Antibody-antigen interaction
    Optional: test expression + antibody/antigen interaction test

    ProteoGenix offers a fully customizable antibody sequencing service with high guarantees. With 16 years of experience, our services are exhaustive and standardized and featured as follow for optimal performances:

    • Length of the sequence: antibody variable domains (VH, VL), full-length antibody (VH, VL, Fc)
    • Optional:
    • Deliverables:
      • A complete report including the CDR sequence annotated,
      • Plasmid containing the sequence (if requested),
      • Up to 2mg recombinant antibody (if requested).

    Case study: DNA sequencing of variable
    regions of hybridoma cell line

    Aim of the study

    Here is an example of a customer requesting to sequence a hybridoma cell line we had previously developed for him.

    Materials

    • PacBio RS II
    • Hybridoma cells provided by ProteoGenix
    • TaKaRa MiniBEST Universal RNA Extraction Kit
    • PrimeScript RT reagent Kit with gDNA Eraser
    • TA-cloning Kit

    Hybridoma sequencing protocol

    Total RNA was extracted from hybridoma cells and cDNA subsequently synthesized. Antibody variable genes were then amplified by isotype-specific PCR, separately sub-cloned into a standard cloning vector and sequenced.

    Isotype determination and antibody sequence analysis

    Antibody isotype determination is performed by gel electrophoresis. Details of the protocol are described in the complete report provided in PDF file.

    Results of the sequencing are based on the analysis of at least 10 positive clones and include:

    • Gene sequence
    • Amino acid sequence with annotated CDR

    After completion of the antibody sequencing work, we were asked to engineer a chimeric version, synthesize both antibody cDNAs, subclone them into an expression vector, produce them in XtenCHO cells and determine antibody binding affinity (KD determination) of all 3 antibodies. The aim was to confirm that the chimeric recombinant antibody functionality at least remained equivalent to the mouse recombinant antibody and the mouse hybridoma antibody.

    After confirmation, we were then able to develop humanized antibodies as well as bispecific antibodies for human therapeutic purposes.

    Click on the button below to get the complete report “Parental Antibody Sequencing from Hybridoma”

    Why should you sequence your antibody?

    Patent your antibody sequence
    • Patent application

    The development of a monoclonal antibody can be the result of years of research. Demonstrating the uniqueness of your sequence is the first step to patenting it and securing your past and future investments.

    Antibody engineering
    • Antibody engineering

    Antibody sequencing provides valuable data for further engineering modifications. For example, antibody humanization or affinity maturation can be performed once your mAb sequence has been obtained. Also obtain access to various antibody formats such as bispecific antibodies or antibody-drug conjugates.

    Recombinant antibody production
    • Recombinant production

    Therapeutic antibody development requires a stable cell line able to produce a high amount of stable antibodies without contaminants. Accessing your antibody sequence allows you to benefit from the advantages of recombinant antibody production: high reproducibility, ease of scalability and animal free production

    Hybridoma sequence safety
    • Safety

    Hybridoma cell lines can be lost due to gene loss, gene mutation or contamination. Your hybridoma cell line sequence is the guarantee that you can continue recombinant antibody production even if your hybridoma line is lost.

    What comes after your antibody
    sequence analysis?

    With more than 15 years of experience in antibody development and production, ProteoGenix can offer you far more than your hybridoma sequence. Using our antibody sequencing service can be the start of a long and fruitful collaboration that will significantly increase your chance to go to the clinic. ProteoGenix offers a one-stop solution tailored to take your project further, such as:

    Therapeutic antibody development

    Therapeutic antibody
    development

    ProteoGenix offers high-quality engineering solutions to optimize the properties of your therapeutic antibodies including antibody humanization, affinity maturation, bispecific antibodies, antibody drug conjugates etc..

    Guaranteed recombinant antibody expression

    Recombinant
    antibody production

    ProteoGenix provides one of the highest performing recombinant antibody production platforms in the world thanks to 16 years of experience and proprietary technologies like XtenCHOTM.