With more than 15 years of experience in custom gene synthesis, gene subcloning, recombinant protein production, and antibody development, 90+ technical experts and PhD account managers with deep domain expertise in gene synthesis services resulting from over a decade of experience, and state of the art equipment, ProteoGenix has demonstrated its core expertise in this space.
Our high success rate in protein and antibody production also relies on the quality of our gene sequence engineering work and codon optimization service, both of which are based on strong empirical data, while providing cost effective, one-stop-shop solutions.
Multiple accreditations, 700+ customers and over 85% repeat business for gene synthesis alone, is a true reflection of the confidence that customers place in us. We have a diverse customer base including academia – universities, public research laboratories, as well as industry, including pharmaceuticals, laboratories, diagnostics, veterinary, agri-food industry etc. We offer customer-focused and flexible gene synthesis solutions. With rapid turnaround times (<7 days) and competitive pricing starting from 0.15€/bp we ensure your satisfaction. Our featured publications cited below are illustrative of successful case studies of our genes having been utilized.
Free codon optimization using ProteoGenix’s proprietary, high efficiency, codon optimization algorithm, choice of vector used, site-directed mutagenesis and gene variant libraries are some of our unique differentiators. We have a library of cloning vectors and offer you the flexibility of using your own vector as well. Our existing gene libraries of several dozens of pre-synthesized, human wild type genes drive significant cost and process efficiencies. Gene synthesis has been widely used for improving the expression of genes and has leveraged expensive techniques such as RT-PCR, RACE etc. Fast and reliable access to multiple cDNA sequences, the removal of unwanted restriction sites by the introduction of desired mutations, and the ability to drive speedy and effective antibody drug discovery, not only through rapid gene synthesis services, but by leveraging our high throughput platform for protein synthesis as well, define The ProteoGenix Advantage.
Buy Gene Synthesis
As a leading gene synthesis company, ProteoGenix has developed the following services to meet our customers’ requirements.
|GENE SYNTHESIS PRICES|
|<0.5kb||0.5-1.5kb||1.5-3kb||<0.3 kb||0.3-1.5kb||Up to 1250bp|
|Lead time||9||12||14 to 16||<7||7||12|
|Gene synthesis price per base||Min. 70€||0.18€/bp||Upon request||Starting at 105 €|
We can provide you with custom gene synthesis solutions, from short sequences to very long (up to 40kb) genes. To order gene synthesis fragments >3kb, don’t hesitate to contact us. ProteoGenix is known for its completion of projects for a wide range of complexity levels of sequences with high or low GC content. Our cost-effective pricing and short turnaround times make us the partner of choice for leading research institutions across the globe. Over 15 years of gene synthesis services experience provide our clients opportunities to create any custom synthetic DNA sequence.
Our guaranteed gene synthesis
Any gene in any vector
Delivery of 5µg of lyophilized gene in a pUC57, pUC-SP or pBluescriptIISK+ vector included. If that does not suffice, our seamless technology can efficiently and accurately insert your gene in any vector of your choice.
Our competitive custom gene synthesis prices supported by fast turnaround times rival the cost of materials like oligonucleotides and molecular biology reagents.
We guarantee quality control by running gene mutagenesis to remove errors. All synthesized genes are sequenced; only constructs with exact sequence agreement are shipped.
No win – No fee
Our gene synthesis service is guaranteed. Service Highlight – If product delivered is not as specified, no fee will be taken.
Process to purchase gene synthesis
OLIGO DESIGN AND SYNTHESIS
Our complementary gene synthesis services include
ProteoGenix has developed a unique codon optimization algorithm based on experimental data.
Most of amino acids are encoded by multiple codons that are called synonymous codons. However, specific codons are used more often than others during gene translation. This phenomenon is called the codon usage bias and is species specific. So, as a result of the redundancy of the genetic code, rare codons can be replaced by codons that are more abundant in the genes of the host organism, without changing the amino acid sequence of the protein, per se. ProteoGenix’s Codon Optimization Tool leverages a codon bias strategy and additional parameters like GC content, repetitive sequences, avoiding restriction sites and mRNA secondary structure.
Applications of codon optimization include:
Ease of DNA Synthesis: ProteoGenix’ Complimentary Codon Optimization Tool, while accounting for natural codon bias and synthesis complexity, significantly enhances the efficiency of designing customized synthetic genes.
Recombinant protein production in a repertoire of expression systems: Codon optimization is essential for recombinant protein production as it allows easy gene subcloning steps and enables gene expression as well as yields improvement.. The heterologous expression of recombinant proteins is a critical aspect of modern biotechnology and biomedicine.
Gene therapy: Gene therapy, leveraging codon optimization, is being applied to treat multiple conditions including Duchene muscular dystrophy (Athanasopoulos, T et al, 2011) and Hepatitis C (Frelin L et al, 2004).
Genetic immunization: An immune response can be obtained by injecting the gene encoding the antigen directly into the animals. In this way, DNA vaccine is a perfect tool when a protein is difficult to express or purify. Genetic immunization using DNA vaccines requires the high expression of the gene of interest. It has been demonstrated that wild type genes generate a low or moderate levels of immune response as compared to genes that have been codon-optimized (Stratford et al., 2000; Uchijima et al., 1998). In addition, while adjuvants are known to enhance the immune responses in the case of recombinant protein vaccines, no such adjuvants are in the case of DNA vaccines, resulting in the poor performance of the DNA vaccines in larger animals. Engineering CpG islands into DNA vaccines has on the other hand enhanced the immunogenicity of DNA vaccines (Krieg et al., 1998). Thus, customized DNA synthesis and gene assembly can add considerable value.
ProteoGenix can provide gene variant libraries with changes in one or several positions to optimize protein expression. This service can be very useful for high-throughput screening for applications such as protein engineering (change/mutation of AAs in one or several positions).
As a leading gene synthesis manufacturer, ProteoGenix also offers endotoxin free plasmid amplification services. Indeed, after gene optimization and gene cloning, it is usually necessary to amplify the construction in order to have endotoxin free plasmid available in sufficient amounts for experiments.
Why choose ProteoGenix for your gene synthesis?
More than 85% of our clients return for 2 or more orders.
<48hr Response Time
Our end-to-end support from the start of the project to product shipping as well as our comprehensive downstream services will assure you receive your product on time.
PhD- scientists with strong molecular biology backgrounds including 30+ technical experts with more than 3 years experience and team leaders with more than 10 years experience in gene synthesis can help tailor the order to the desired specifications, and support you through the project.
>10 000 gene constructs synthesized
Benefit from our experience with universities, public research laboratories and with private companies (pharmaceutical laboratories, diagnostics, veterinary and agri-food companies)
Fast Worldwide delivery
Have your desired gene construct delivered to your doorstep anywhere in the world with standard turnaround times starting at less than 7 days.
Applications of custom gene synthesis
Immunology and antibody discovery
Recombinant antibodies can be synthetically engineered to reduce immunogenicity while enhancing specificity, stability, cross-reactivity and affinity, for increased safety and efficacy. Monoclonal antibodies are the new generation biotherapeutics.
Synthetic biology applies custom gene synthesis and utilizes it to build artificial biological systems for research, engineering and medical applications. The CRISPR/Cas9 system for gene editing has been hailed by The Washington Post as “the most important innovation in the synthetic biology space in nearly 30 years”.
Agrigenomics research is being emphasized to optimize agricultural productivity and nutrition.
The use of biomarkers and companion diagnostics as neuro/chemical/biological indicators in research are driving the onset of precision medicine. Medical practitioners depend on patients’ pharmacogenomic profile to detect genetic disorders in them.
Custom gene synthesis advancements have seen a major increase in the data produced by cancer research initiatives. Using Next Generation Sequencing and whole genome targeting scientists are better understanding protein functioning in oncogenesis.
Phage display is a powerful tool to screen antibody related DNA libraries. Generating DNA libraries for antibody engineering requires strong molecular biology skills to generate relevant cDNA to build a powerful library.
Gene synthesis testimonial
” The optimized genes ordered had an excellent quality, were cost-effective and perfectly adapted for biochemical and biophysical studies. In addition, the account manager in charge of the service advised us really well before and after the sales process, and we got the constructs on time. “
Gene synthesis: featured publications
Bicc1 Polymerization Regulates the Localization and Silencing of Bound mRNA
Benjamin Rothé,a Lucia Leal-Esteban,a Florian Bernet,a Séverine Urfer,a Nicholas Doerr,b Thomas Weimbs,b Justyna Iwaszkiewicz,c and Daniel B. Constama
Fine Mapping and Characterization of the L-Polymerase-Binding Domain of the Respiratory Syncytial Virus Phosphoprotein
Julien Sourimant,a,c Marie-Anne Rameix-Welti,a,c,d Anne-Laure Gaillard,a Didier Chevret,b Marie Galloux,a Elyanne Gault,c,d and Jean-François Eléouëta
Human CalDAG-GEFI gene (RASGRP2) mutation affects platelet function and causes severe bleeding
Matthias Canault,1,2,3 Dorsaf Ghalloussi,1,2,3 Charlotte Grosdidier,1,2,3 Marie Guinier,4 Claire Perret,5,6,7 Nadjim Chelghoum,4 Marine Germain,5,6,7 Hana Raslova,8 Franck Peiretti,1,2,3 Pierre E. Morange,1,2,3 Noemie Saut,1,2,3 Xavier Pillois,9,10 Alan T. Nurden,10 François Cambien,5,6,7 Anne Pierres,3,11,12 Timo K. van den Berg,13 Taco W. Kuijpers,13 Marie-Christine Alessi,1,2,3 and David-Alexandre Tregouet5,6,7
Interaction of a Partially Disordered Antisigma Factor with Its Partner, the Signaling Domain of the TonB-Dependent Transporter HasR
Idir Malki,1,2,3 Catherine Simenel,1,2 Halina Wojtowicz,1,2 Gisele Cardoso de Amorim,1,2 Ada Prochnicka-Chalufour,1,2 Sylviane Hoos,4 Bertrand Raynal,4 Patrick England,4 Alain Chaffotte,1,2 Muriel Delepierre,1,2 Philippe Delepelaire,5 and Nadia Izadi-Pruneyre1,2,*
Kinetics of nif Gene Expression in a Nitrogen-Fixing Bacterium
César Poza-Carrión, Emilio Jiménez-Vicente, Mónica Navarro-Rodríguez, Carlos Echavarri-Erasun, and Luis M. Rubio
Bacterial cytoplasm as an effective cell compartment for producing functional VHH-based affinity reagents and Camelidae IgG-like recombinant antibodies
Selma Djender, Aurelie Schneider, Anne Beugnet, Ronan Crepin, Klervi Even Desrumeaux, Chiara Romani, Sandrine Moutel, Franck Perez, and Ario de Marco
Trypanosoma vivax GM6 Antigen: A Candidate Antigen for Diagnosis of African Animal Trypanosomosis in Cattle
Davita Pillay,1,* Julien Izotte,1 Regassa Fikru,2,3,4 Philipe Büscher,3 Hermogenes Mucache,5 Luis Neves,5 Alain Boulangé,5,7 Momar Talla Seck,6 Jérémy Bouyer,6,7,8Grant B. Napier,9 Cyrille Chevtzoff,10 Virginie Coustou,1 and Théo Baltz1
α-Catenin and Vinculin Cooperate to Promote High E-cadherin-based Adhesion Strength*
William A. Thomas,a,b Cécile Boscher,c,d,1 Yeh-Shiu Chu,e,2 Damien Cuvelier,f Clara Martinez-Rico,b Rima Seddiki,c,d,g Julie Heysch,b Benoit Ladoux,g,h Jean Paul Thiery,e,h,i,j,3 René-Marc Mege,c,d,3 and Sylvie Dufourb,3,4
Structural, Expression and Interaction Analysis of Rice SKP1-Like Genes
Senda Kahloul,1,2 Imen HajSalah El Beji,1 Aurélia Boulaflous,1 Ali Ferchichi,2 Hongzhi Kong,3 Said Mouzeyar,1,* and Mohamed Fouad Bouzidi1
Unique Features of a Pseudomonas aeruginosa α2-Macroglobulin Homolog
Mylène Robert-Genthon,a,b,c,d Maria Guillermina Casabona,a,b,c,d David Neves,e Yohann Couté,a,c,d Félix Cicéron,a,b,c,d* Sylvie Elsen,a,b,c,d Andréa Dessen,b,c,d,e and Ina Attréea,b,c,d
Recognition of Multivalent Histone States Associated with Heterochromatin by UHRF1 Protein
Nataliya Nady,‡ Alexander Lemak,‡,1 John R. Walker,§,1 George V. Avvakumov,§,1 Michael S. Kareta,¶,1 Mayada Achour,‖ Sheng Xue,§ Shili Duan,‡ Abdellah Allali-Hassani,§ Xiaobing Zuo,* Yun-Xing Wang,* Christian Bronner,‖ Frédéric Chédin,¶ Cheryl H. Arrowsmith,‡§,2 and Sirano Dhe-Paganon§‡‡,