With more than 13 years of experience (since 2003) in gene synthesis, subcloning, recombinant protein production, and antibody development, ProteoGenix perfectly understands how important gene optimization, synthesis and subcloning are for the development of reagents. Our high success rate in protein and antibody production also relies on the quality of our gene sequence engineering work and codon optimization service, both of which are based on strong empirical data.

 

Our Gene synthesis services:





ProteoGenix has developed four different gene synthesis services to meet all our customers' requirements.

STANDARD
EXPRESS
PCR FRAGMENTS
<0.5
kb
>0.5-
1,5kb
>1.5-
3kb
<0.5
kb
<0.5-
1kb
>1kb
2kb
Up to 1250bp
Lead time
(business days)
±9 ±12 ±14
-16
<7 7 12 -
Price Min. 140€ 0.28€/bp Upon request Starting at
105€

We’re able to synthetize from short to very long (>10 000bp) genes, for any gene synthesis request >3kb don’t hesitate to contact us.


ProteoGenix guarantees:

  • Double-stranded cDNA synthesis
  • Sequence delivered = 100% accuracy guaranteed by quality control
  • Sequencing
  • Efficiency >99.9%
  • Gene Mutagenesis run to remove errors
  • Delivery of 5µg of lyophilized gene in a pUC57, pUC-SP or pBluescriptIISK+ vector included

    -> Or any vector of your choice

 

Complementary services:

  • Free codon optimization
  • ProteoGenix has developed a unique codon optimization algorithm based on experimental data
What is codon optimization?
Each living species has a different preference for codon usage. The redundancy of the genetic code allows the exchange of rare codons for the most widely used codons encoding the same amino acid. For recombinant protein production purpose, this exchange permits a better expression of the protein in the chosen organism.

Codon optimization is widely used for:

Recombinant protein production in various expression systems
Cell transfection
Gene therapy
Genetic immunization

 

  • Sub-cloning: into one of our vector or directly into yours
  • Site-directed mutagenesis from any existing template provided
  • Gene variant libraries with particular changes in one or more designed position(s)
ProteoGenix can provide gene variant libraries with changes in one or several positions. This service can be very useful for high-throughput screening for applications such as protein engineering (change/mutation of AAs in one or several positions), and high quality plasmid preparation, including endotoxin-free plasmids for transformation, transfection, or in vitro transcription

 

  • Storage

Request a quote

Featured Publications

Bicc1 Polymerization Regulates the Localization and Silencing of Bound mRNA
Benjamin Rothé,a Lucia Leal-Esteban,a Florian Bernet,a Séverine Urfer,a Nicholas Doerr,b Thomas Weimbs,b Justyna Iwaszkiewicz,c and Daniel B. Constama

Fine Mapping and Characterization of the L-Polymerase-Binding Domain of the Respiratory Syncytial Virus Phosphoprotein
Julien Sourimant,a,c Marie-Anne Rameix-Welti,a,c,d Anne-Laure Gaillard,a Didier Chevret,b Marie Galloux,a Elyanne Gault,c,d and Jean-François Eléouëta

Human CalDAG-GEFI gene (RASGRP2) mutation affects platelet function and causes severe bleeding
Matthias Canault,1,2,3 Dorsaf Ghalloussi,1,2,3 Charlotte Grosdidier,1,2,3 Marie Guinier,4 Claire Perret,5,6,7 Nadjim Chelghoum,4 Marine Germain,5,6,7 Hana Raslova,8 Franck Peiretti,1,2,3 Pierre E. Morange,1,2,3 Noemie Saut,1,2,3 Xavier Pillois,9,10 Alan T. Nurden,10 François Cambien,5,6,7 Anne Pierres,3,11,12 Timo K. van den Berg,13 Taco W. Kuijpers,13 Marie-Christine Alessi,1,2,3 and David-Alexandre Tregouet5,6,7

Interaction of a Partially Disordered Antisigma Factor with Its Partner, the Signaling Domain of the TonB-Dependent Transporter HasR
Idir Malki,1,2,3 Catherine Simenel,1,2 Halina Wojtowicz,1,2 Gisele Cardoso de Amorim,1,2 Ada Prochnicka-Chalufour,1,2 Sylviane Hoos,4 Bertrand Raynal,4 Patrick England,4 Alain Chaffotte,1,2 Muriel Delepierre,1,2 Philippe Delepelaire,5 and Nadia Izadi-Pruneyre1,2,*

Kinetics of nif Gene Expression in a Nitrogen-Fixing Bacterium
César Poza-Carrión, Emilio Jiménez-Vicente, Mónica Navarro-Rodríguez, Carlos Echavarri-Erasun, and Luis M. Rubio

Bacterial cytoplasm as an effective cell compartment for producing functional VHH-based affinity reagents and Camelidae IgG-like recombinant antibodies
Selma Djender, Aurelie Schneider, Anne Beugnet, Ronan Crepin, Klervi Even Desrumeaux, Chiara Romani, Sandrine Moutel, Franck Perez, and Ario de Marco

Trypanosoma vivax GM6 Antigen: A Candidate Antigen for Diagnosis of African Animal Trypanosomosis in Cattle
Davita Pillay,1,* Julien Izotte,1 Regassa Fikru,2,3,4 Philipe Büscher,3 Hermogenes Mucache,5 Luis Neves,5 Alain Boulangé,5,7 Momar Talla Seck,6 Jérémy Bouyer,6,7,8 Grant B. Napier,9 Cyrille Chevtzoff,10 Virginie Coustou,1 and Théo Baltz1

α-Catenin and Vinculin Cooperate to Promote High E-cadherin-based Adhesion Strength*
William A. Thomas,a,b Cécile Boscher,c,d,1 Yeh-Shiu Chu,e,2 Damien Cuvelier,f Clara Martinez-Rico,b Rima Seddiki,c,d,g Julie Heysch,b Benoit Ladoux,g,h Jean Paul Thiery,e,h,i,j,3 René-Marc Mege,c,d,3 and Sylvie Dufourb,3,4

Structural, Expression and Interaction Analysis of Rice SKP1-Like Genes
Senda Kahloul,1,2 Imen HajSalah El Beji,1 Aurélia Boulaflous,1 Ali Ferchichi,2 Hongzhi Kong,3 Said Mouzeyar,1,* and Mohamed Fouad Bouzidi1

Unique Features of a Pseudomonas aeruginosa α2-Macroglobulin Homolog
Mylène Robert-Genthon,a,b,c,d Maria Guillermina Casabona,a,b,c,d David Neves,e Yohann Couté,a,c,d Félix Cicéron,a,b,c,d* Sylvie Elsen,a,b,c,d Andréa Dessen,b,c,d,e and Ina Attréea,b,c,d

Recognition of Multivalent Histone States Associated with Heterochromatin by UHRF1 Protein
Nataliya Nady, Alexander Lemak,‡,1 John R. Walker,§,1 George V. Avvakumov,§,1 Michael S. Kareta,¶,1 Mayada Achour, Sheng Xue,§ Shili Duan, Abdellah Allali-Hassani,§ Xiaobing Zuo,** Yun-Xing Wang,** Christian Bronner, Frédéric Chédin, Cheryl H. Arrowsmith,‡§,2 and Sirano Dhe-Paganon§‡‡,