Phage Display form

ProteoGenix can perform phage display technique to provide you with the most refined binders possible for your project. The construction of an immune library potentially increases the affinity of your final antibody against the targeted protein.

 

An immune library requires prior immunization, which takes longer than performing a phage display screening from a naive library.

 

checkAny kind of animal species available

checkHigh affinity antibodies

Immune library construction service

 

ProteoGenix is an expert in antibody production so many antibody libraries from various species can be generated at your convenience: Rabbit, Mouse, Sheep or even Alpaca and Llama (for VHH libraries).

 

Immunization 8 weeks ; Isolation of PBMC + spleen + bone marrow, Total RNA extraction, cDNA synthesis in 3/4 weeks ; PCR VH and VL amplification in 3/4 weeks ; Construction of the library phagemid synthesis and cloning, Transformation for production in 2 weeks and TOTAL : 15/18 weeks

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Advantages of using an immune library for a specific project

  • Provides binders with a higher affinity compared to a naive library

  • More antibodies directed against the antigen of interest

  • Ideal for small and modified antigens (specific antibody with low capacity)

  • Allows work with any species as there is no fusion necessary compared to hybridoma technique

    (example for rabbit monoclonal antibodies)


Construction and screening of an immune library

 

The library capacity is measured at 109 to 1016 different binders. We are able to offer you several types of libraries: scFv, Fab and VHH. The library screening is performed to identify binders with the best affinity.
Construction and screening of an immune library can be tailored to your research for high specificity and stability.

The screening process is based on our standard protocol of panning/biopanning and ProteoGenix can adapt this protocol to any request. You can find out more about the stages of biopanning in the screening from a naive library.

After 3 to 5 rounds of biopanning, ProteoGenix provides you with several binders (3 to 10) whilst giving you the option to ask for more if required.

 

Chart of panning (biopanning) process : 1 - Incubation with phage library 2 - Wash away non-binders 3 - Elution of binders 4 - 3 to 5 rounds of panning/biopanning process 5 - ELISA to determine specificity of binding after 3-5 rounds of biopanning
Chart of panning (biopanning) process with Phage display. 1 – Incubation with phage library 2 – Wash away non-binders 3 – Elution of binders 4 – 3 to 5 rounds of panning/biopanning process 5 – ELISA to determine specificity of binding after 3-5 rounds of biopanning

 

At ProteoGenix, the choice of building and screening an immune library by phage display will allow you to produce monoclonal antibodies
with the highest affinity for any species.

For more information or to get a quote for our phage display services with the use of immune libraries, don’t hesitate to contact us.

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