ProteoGenix can perform phage display technique to provide you with the most refined binders possible for your project. The construction of an immune library potentially increase the affinity of your final antibody against the targeted protein.
An immune library requires prior immunization, which takes longer than performing a phage display screening from a naive library.
Any kind of animal species available
High affinity antibodies
ProteoGenix is an expert in antibody production so many antibody libraries from various species can be generated at your convenience: Rabbit, Mouse, Sheep or even Alpaca and Llama (for VHH libraries).
(example for rabbit monoclonal antibodies)
The library capacity is measured at 109 to 1016 different binders. We are able to offer you several types of libraries: scFv, Fab and VHH. The library screening is performed to identify binders with the best affinity.
Construction and screening of immune library can be tailored to your research for high specificity and stability.
The screening process is based on our standard protocol of panning/biopanning and ProteoGenix can adapt this protocol to any request. You can find out more about the steps of biopanning in the screening from a naive library.
After 3 to 5 rounds of biopanning, ProteoGenix provides you with several binders (3 to 10) while allowing you to ask for more if you wish.
Chart of panning (biopanning) process with Phage display. 1 - Incubation with phage library 2 - Wash away non-binders 3 - Elution of binders 4 - 3 to 5 rounds of panning/biopanning process 5 - ELISA to determine specificity of binding after 3-5 rounds of biopanning
The choice of building and screening an immune library by phage display at ProteoGenix will allow you to produce monoclonal antibodies
with the highest affinity for any species.
For more information or to get a quote for our phage display services with the use of immune libraries, don’t hesitate to contact us.
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