Mammalian cells are in most cases the optimal system to produce mammalian proteins as the resulting recombinant proteins are very close to the native form. However, production yields are often lower than in other systems and require much greater effort to get good amounts of purified proteins.

Our skills in protein production and engineering, especially in mammalian cells, are wide, and include the following custom recombinant protein services:

  • Basic expression evaluation of recombinant proteins and antibodies
  • High throughput expression condition screening
  • Antibody humanization
  • Production in flasks and in bioreactors of up to 120L
  • Bi-specific antibody production
  • Stable cell line development
  • Process development optimization
  • Master and working cell bank production
  • Stable isotopically labeled recombinant protein production

 

Standard mammalian cells protein expression package

  • Project study
  • Endotoxin-free plasmid preparation
  • Mammalian cells transfection
  • Transient expression evaluation in 80 ml
  • SDS/PAGE analysis and WB if required
  • Purification tests
  • SDS/PAGE analysis and WB if required
  • 1L expression and purification scale up
  • SDS/PAGE analysis and WB if required
 
  • Final detailed report
  • Purified protein
 
  • 7 - 9 weeks

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