As a leader in antibody production, ProteoGenix offers and develops a wide range of innovative services based on the phage display technique.

 

Large size libraries up to 1016

Short delivery time

3 to 10 binders guaranteed

Full rights transferred to customers

 

 

Our extensive expertise allows us to provide binders with the best possible features in terms of affinity, specificity, functionality, stability to meet your projects requirements.


Naive library vs immune library in phage display?

Naive library screening

  • Screening of a pre-established antibody library
    against the desired antigen.
  • Human, Rabbit (coming soon), Mouse, (scFv and Fab libraries)
    Alpaca, Camel (VHH libraries)
  • IDEAL FOR NON STANDARD LABORATORY ANIMALS SPECIES
    OR FOR HUMAN
  • 7 weeks
    No animal use
  • Cheaper

Immune library generation and screening

  • ProteoGenix starts with animal immunizations, followed by spleen cells and bone marrow collection, RNA extraction, antibody hybridization and library preparation.
  • Rabbit, Mouse, Sheep
    Alpaca, Camel (VHH libraries)
  • IDEAL FOR SMALL
    AND MODIFIED ANTIGENS
  • 15-18 + 7 weeks
    Animal injections
  • More expensive

Depending on your needs, ProteoGenix proposes to use a naive library or immune library with up to 1016 available clones.


Monoclonal antibody humanization service

 

A key advantage of phage display is that for bio therapeutic antibodies, we don’t need to perform any humanization as the library that we screen can be already human. In sharp contrast, monoclonal antibodies developed by the classical hybridoma method or via phage display usually require humanization procedures. And this is not always straight forward as changing the germline of the antibody and performing CDR grafting on it can affect some of its key features. Feel free to consult our various services concerning the humanization of monoclonal antibodies.


Affinity maturation service in phage display

 

Once best binders are identified, they are sequenced, subcloned and expressed for further characterization in different applications, which can potentially be followed by affinity maturation. ProteoGenix is producing highly specific and high affinity antibodies from various species including camel, alpaca, rabbit, mouse, sheep and human. But in some cases, affinity expectations are high enough to justify further antibody optimization. We therefore generate a new library by site directed mutagenesis followed by a new biopanning to select high affinity binders.


Additional sequence optimization services offered by ProteoGenix

 

If you want to know more, ProteoGenix usually engineers the sequence to increase the production yield of the antibody, optimize the stability of the molecule and increase its affinity (affinity maturation service).

Through the process of therapeutic antibodies development, it is important to optimize the molecule at this stage as it is impossible to modify the sequence after the first validation studies have started. However, optimizing the sequence is the most efficient way to tackle, yield, affinity and stability issues. Our experts will study the sequence and optimize some amino acids or motifs to generate up to 60 variants that will be tested and selected to meet your expectations. 3D modeling analysis supports our work to make the most accurate and relevant change in the sequences.

Don’t hesitate to contact us for further information or to get a free quote.

Featured applications of phage display with ProteoGenix – VHH antibodies and rabbit monoclonal antibodies

 

At ProteoGenix, several services related to the technique of phage display are offered to you: humanization of monoclonal antibodies, production of rabbit monoclonal antibodies (via the phage display technique) but also the production and the use of VHH antibodies, (attracting enormous interest due to their small size, which makes them penetrate the cells more easily) of which we own specific libraries developed from alpaca.


What is phage display?

 

The phage display technology involves the determination of interaction partners of a protein (protein-protein, protein-DNA or protein-peptide interactions), which would be used as the “bait” for phages expressing peptides, proteins or antibodies coded by a huge DNA library (consisting of all coding sequences of a given organism). The function of that protein may therefore be determined.