Protein expression

Protein Production Form

We are your protein expression experts
1500+ PROTEINS5 SYSTEMS
We are your protein expression experts
1500+ PROTEINS
5 SYSTEMS

With more than 15 years of extensive experience in custom protein expression, and over 1500 proteins successfully produced in 5 systems, ProteoGenix is a leader in difficult-to-express recombinant proteins and antibodies productions.  READ MORE

Recombinant protein synthesis services
Difficult-to-express protein production experts

We provide small to large-scale protein expression and purification services in each of or 5 platforms at competitive prices but also more advanced and customized packages in which we test for instance custom recombinant protein expression conditions in several systems in parallel or with extended protein production conditions in a given system. Our services include all the steps from gene synthesis and expression vector construction to pilot protein production in flasks and large-scale protein expression in fermenters and bioreactors.

Our High throughput protein expression condition screening is intended to overcome challenges related to solubility, purity, functionality, productivity, refolding or formulation. Such a commitment involves having access to, and mastering, all existing technologies, processes and tools available. With extensive experience in the expression of recombinant proteins of any kind, such as antibodies, enzymes, membrane proteins, cytokines, etc., and of any species, ProteoGenix has developed a complete toolbox to tackle any such challenge and to provide best-in-class protein engineering services. Try guaranteed packages from the most reliable protein production company of its kind.

E. coli

Protein production in E. coli

PROTEIN
EXPRESSION IN E. COLI

Periplasmic expression, Refolding tests, guaranteed
packages, endotoxin removal, Custom E. coli expression,
protein production in bacteria

READ MORE

Mammalian

Protein production in Mammalian cells

PROTEIN EXPRESSION
IN MAMMALIAN CELLS

Transient expression, Recombinant antibody
production, SEC and IEX purifications,
Low endotoxin levels, CHO, HEK293, BHK

READ MORE

RAB

Recombinant antibody production services

RECOMBINANT
ANTIBODY PRODUCTION

Low cost production,
guaranteed yield,
HTP antibody variants

READ MORE

Stable cell line

Stable cell line development

STABLE CELL LINE
DEVELOPMENT

IP free systems, guaranteed yield, process
development, HEK293 and CHO stable
cell line

READ MORE

HTP

High Throughput protein expression screening

HIGH THROUGHPUT PROTEIN
EXPRESSION SCREENING

For difficult-to-express proteins, 1000+
expression conditions, small-scale, high
throughput screening

READ MORE

Insect cells

Protein production in Insect cells

INSECT CELLS
PROTEIN EXPRESSION

Sf9, Sf21, Hi5, glycosylated proteins,
protein purification, baculovirus
production

READ MORE

B. subtilis

Protein production in B.subtilis

PROTEIN
PRODUCTION IN B. SUBTILIS

Low endotoxin level,
Recombinant protein secretion,
protein characterization

READ MORE

Yeast

Protein production in Yeast

YEAST
PROTEIN EXPRESSION

Saccharomyces cerevisia,
Pichia pastoris,
stable expression

READ MORE

Multi system

Multi-system protein expression

MULTI-SYSTEM
EXPRESSION PACKAGES

For unknown optimal system, tests in up
to 5 systems, purified protein delivery for
each system

READ MORE

Large scale

Large scale protein production in fermenter and bioreactor

FERMENTATION / BIOREACTOR
LARGE – SCALE PROTEIN PRODUCTION

1 to 4000L in each 5 systems,
Large scale purification services,
Process development services

READ MORE

Custom Protein Production
Testimonial

” I recently chose ProteoGenix to produce a recombinant protein in HEK293 cells. The high productivity and strong activity of the protein obtained, probably due to proper glycosylation and folding, allowed us to generate exciting results in functional studies and reach new milestones in our research project. The quality of the service provided, and the smooth and kind communication I had with the account manager in charge of our project, were paramount to this success. I’m happy to recommend ProteoGenix for recombinant protein productions. “

production_portrait01
Dr Christian BRONNER,
PhD and principal
scientist at CNRS

Why Choose ProteoGenix for your Protein Expression?

Process development experience
 More than 16 years of outstanding track record in the production of difficult-to-express proteins
5 protein expression systems
5 available protein expression systems and complete toolbox to overcome any particular protein expression challenges
Onestop solution from gene to protein
One-stop solution from gene to protein, process development and large scale production. Consequences: faster service, increased success rate, less administrative work.
IP Free strains and cell lines
IP-free strains and cell lines
transient protein expression service
Very small to large scale productions with 1 to 4000 L batch
Transient expression and stable cell line
Productivity of up to 800mg/L in transient expression and 5g/L in stable expression

Why Choose ProteoGenix
for your Protein Expression ?

Process development experience

 More than 14 years of outstanding track record in the production of difficult-to-express proteins

5 protein expression systems

5 available protein expression systems and complete toolbox to overcome any particular protein expression challenges

5 protein expression systems

One-stop solution from gene to protein, process development and large scale production. Consequences: faster service, increased success rate, less administrative work.

IP Free strains and cell lines

 IP-free strains and cell lines

transient protein expression service

Very small to large scale productions with 1 to 4000 L batch

Transient expression and stable cell line

Productivity of up to 800mg/L in transient expression and 5g/L in stable expression.

Fermentation facilities

Protein production in bacteria, yeast, insect, mammalian cells
Process development in bioreactors

Case Study:

Claudiximab protein expression

A customer requested the recombinant expression of Claudiximab. He ordered a protein expression package including:

  • Gene synthesis with codon optimization
  • Subcloning in an expression vector
  • Small-scale protein expression and purification

GENE SYNTHESIS AND SUBCLONING

The genes coding for the Heavy chain (HC) and the light chain (LC) of the therapeutic antibody Claudiximab were chemically synthesized with optimization for protein expression in mammalian cells. The genes were cloned in ProteoGenix’s proprietary mammalian cells expression vector pTXs1.

PROTEIN EXPRESSION

recombinant protein expression purification

Figure 1. Purification profile of Claudiximab. Reduced PAGE with Coomassie blue staining.
IN. Input sample. FT. Flow through. W. Wash step. E1-E11. Eluted fractions.

protein production QC

Figure 2. Final QC of purified Claudiximab. Coomassie blue staining.
A. Non-reduced PAGE. B. Reduced PAGE

PROTEIN EXPRESSION RESULT

Production YieldPurity
Claudiximab1281mg/L≥95%

Which System choose for your Protein Production?

COMPARISON BETWEEN 5 EXPRESSION SYSTEMS PROVIDED BY PROTEOGENIX -
PROKARYOTIC VS EUKARYOTIC CELLS EXPRESSION SYSTEMS.

From a general standpoint, the table below gives an idea of the advantages of each five different heterologous protein expression systems for production of recombinant proteins. Of course, features can vary from one protein to another. The more arrows there are, the better it is.

Expression
system
TimeCostPTMLow Endotoxin levelsSimple process scale upSecretion
E. coli**************
B. subtilis****************
Yeast (P. pastoris,
S. cerevisiae)
**************
Baculovirus/
Insect cells
**************
Mammalian cells******************

The table below gives an idea of the advantages of each five different heterologous protein expression systems for production of recombinant proteins from a general standpoint. Of course features can vary from one protein to another. The more arrows there are, the better it is.

Expression
system
TimeCostPTMLow Endotoxin levels
E. coli**********
B. subtilis***********
Yeast (P. pastoris,
S. cerevisiae)
**********
Baculovirus/
Insect cells
**********
Mammalian cells************
Expression
system
Simple process scale upSecretion
E. coli****
B. subtilis*****
Yeast (P. pastoris,
S. cerevisiae)
****
Baculovirus/
Insect cells
****
Mammalian cells******

TIME DURATION AND PRICE

Expression systemTime durationPrice
E. coli3-6 weeksStarting from
1 650€
B. subtilis4-6 weeks
Yeast (P. pastoris,
S. cerevisiae)
6-7 weeks
Baculovirus/
Insect cells
8-11 weeks
Mammalian cells7-9 weeks

Service Content
Small scale expression and purification evaluation with many expression conditions tested.
1L production and purification

Package codeTypeSystemsPrice
ATX-PTR4S014 systems
Expression package
E. coli / Yeast / Baculovirus /
Mammalian cells
Starting from
2 450€
ATX-PTR3S053 system
Expression package
B. subtilis/ Yeast / Baculovirus
ATX-PTR2S022 systems
Expression package
E. coli / Baculovirus

  The most commonly used system of recombinant protein purification is E. coli. Indeed, it is the simplest, fastest and most cost effective system for custom expression of a protein. Bacterial cultures grow much faster than insect or mammalian cells or yeast. It is therefore easier and quicker to test a large number of different conditions. More solutions are also available for testing and therefore allow a higher success rate in expressing recombinant proteins. Transient expression yields of recombinant protein are also generally much higher than in yeast, baculovirus / insect cells or mammalian cells. Production of recombinant proteins in E. coli (Escherichia Coli) is often the preferred solution proposed by ProteoGenix when the final application requires large quantities of proteins such as crystallography and nuclear magnetic resonance (NMR) and no Post-Translational Modifications (PTM) are required. However, the main drawbacks of E. coli system are the high amounts of endotoxins remaining in the final sample and the lack of Post-Translational modifications. It is possible to remove endotoxins but it isn’t convenient at an industrial stage when there is not much to be done to solve PTM problems apart from using a different expression system. With these five systems, we are able to meet the requirements of a large number protein synthesis orders and we therefore have a success rate of over 95% in recombinant protein synthesis. We are constantly working on integrating new systems and solutions to provide the best level of service for anyone who needs to buy high quality recombinant protein production services.

Team, Capacity, Organization

  If you have any doubts about which system to choose from, we, as a leading protein production manufacturer, can guide you from gene to protein and help you define an optimal strategy while taking into account your time constraints, budget and required protein features..

  Our recombinant protein expression laboratories are organized across several platforms and supporting labs to ensure the best quality of service, the highest product purity, the lowest risk of contamination and the best organization for a fast and reliable service.

  We have a platform for each of our five systems and a separate quality control lab, molecular biology lab, packing lab… Each platform is managed by experienced team leaders that ensure they deliver proteins corresponding to initial customer specifications. With more than 1500 proteins produced to date, and specialized teams for each system, our expertise is unrivalled.

Custom recombinant protein
expression applications

  ProteoGenix recombinant proteins are used in a wide spectrum of applications and our customers are usually involved in Life sciences, including Pharma companies, Diagnostics, Biotech, the Agri-Food Industry, Crop science, New energies, Cosmetics, National Research Laboratories, etc..

  Below is a non-exhaustive list of applications:

  • Protein involved in diagnostic assays for calibration, positive controls or capture components
  • Production of recombinant antibody variants for therapeutic antibodies humanization
  • Production of food enzymes involved in food production processes or quality controls
  • Recombinant protein antigen for the production of polyclonal or monoclonal antibodies (antigen preparation)
  • Functional and activity studies
  • Crystallography
  • Protein interaction studies
  • Patent issue investigations
  • Mutant engineering studies for drug efficacy improvements in drug discovery