CUSTOM PROTEIN EXPRESSION SERVICES

With more than 15 years of extensive experience in custom protein expression, and over 1500 proteins successfully produced in 5 systems, ProteoGenix is a leader in difficult-to-express recombinant proteins and antibodies productions.

Custom Protein Production
Testimonial

Fermentation facilities

Case Study:

Claudiximab protein expression

A customer requested the recombinant expression of Claudiximab. He ordered a protein expression package including:

• Gene synthesis with codon optimization
• Subcloning in an expression vector
• Small-scale protein expression and purification

Gene Synthesis And Subcloning

The genes coding for the Heavy chain (HC) and the light chain (LC) of the therapeutic antibody Claudiximab were chemically synthesized with optimization for protein expression in mammalian cells. The genes were cloned in ProteoGenix’s proprietary mammalian cells expression vector pTXs1.

Protein Expression

Which System choose for your Protein Production?

Omparison Between 5 Expression Systems Provided By
Proteogenix –
Prokaryotic Vs Eukaryotic Cells Expression Systems.

Service Content
Small scale expression and purification evaluation with many expression conditions tested.
1L production and purification

The most commonly used system of recombinant protein purification is E. coli. Indeed, it is the simplest, fastest and most cost effective system for custom expression of a protein. Bacterial cultures grow much faster than insect or mammalian cells or yeast. It is therefore easier and quicker to test a large number of different conditions. More solutions are also available for testing and therefore allow a higher success rate in expressing recombinant proteins. Transient expression yields of recombinant protein are also generally much higher than in yeast, baculovirus / insect cells or mammalian cells. Production of recombinant proteins in E. coli (Escherichia Coli) is often the preferred solution proposed by ProteoGenix when the final application requires large quantities of proteins such as crystallography and nuclear magnetic resonance (NMR) and no Post-Translational Modifications (PTM) are required. However, the main drawbacks of E. coli system are the high amounts of endotoxins remaining in the final sample and the lack of Post-Translational modifications. It is possible to remove endotoxins but it isn’t convenient at an industrial stage when there is not much to be done to solve PTM problems apart from using a different expression system. With these five systems, we are able to meet the requirements of a large number protein synthesis orders and we therefore have a success rate of over 95% in recombinant protein synthesis. We are constantly working on integrating new systems and solutions to provide the best level of service for anyone who needs to buy high quality recombinant protein production services.

Team, Capacity, Organization

If you have any doubts about which system to choose from, we, as a leading protein production manufacturer, can guide you from gene to protein and help you define an optimal strategy while taking into account your time constraints, budget and required protein features..

Our recombinant protein expression laboratories are organized across several platforms and supporting labs to ensure the best quality of service, the highest product purity, the lowest risk of contamination and the best organization for a fast and reliable service.

We have a platform for each of our five systems and a separate quality control lab, molecular biology lab, packing lab… Each platform is managed by experienced team leaders that ensure they deliver proteins corresponding to initial customer specifications. With more than 1500 proteins produced to date, and specialized teams for each system, our expertise is unrivalled.

Custom recombinant protein
expression applications