null

RECOMBINANT PROTEIN PRODUCTION SERVICES

We are your protein production experts
1500+ PROTEINS5 SYSTEMS
We are your protein production experts
1500+ PROTEINS5 SYSTEMS

Home /  Protein Production

With more than 15 years of extensive experience in custom protein production and over
1500 proteins successfully produced in 5 systems, ProteoGenix is a leader in
difficult-to-express recombinant proteins and antibodies productions.  

READ MORE

Recombinant protein synthesis services

Difficult-to-express protein production experts

We provide small to large-scale protein expression and purification services in each of or 5 platforms at competitive prices but also more advanced and customized packages in which we test for instance custom recombinant protein expression conditions in several systems in parallel or with extended protein expression conditions in a given system. Our services include all the steps from gene synthesis and expression vector construction to pilot protein production in flasks and large-scale protein expression in fermenters and bioreactors.

Our High throughput protein expression condition screening is intended to overcome challenges related to solubility, purity, functionality, productivity, refolding or formulation. Such a commitment involves having access to and mastering all existing technologies, processes and tools available. With an extensive experience in the expression of recombinant proteins of any kinds such as antibodies, enzymes, membrane proteins, cytokines, etc., and of any species, ProteoGenix has developed a very complete toolbox to tackle any such challenge and provide best in class protein engineering services. Try guaranteed packages of the most reliable protein production company of its kind.

E. coli

Protein production in E. coli

PROTEIN
PRODUCTION IN E. COLI

Periplasmic expression, Refolding tests, guaranteed
packages, endotoxin removal, Custom E. coli expression,
protein production in bacteria

READ MORE

Multi system

Multi-system protein expression

MULTI-SYSTEM
EXPRESSION PACKAGES

For Unknown optimal system, tests in up
to 5 systems, purified protein delivery for
each system

READ MORE

B. subtills

Protein production in B.subtilis

PROTEIN
PRODUCTION IN B. SUBTILIS

Low endotoxin level,
Recombinant protein secretion,
protein characterization

READ MORE

Yeast

Protein production in Yeast

PROTEIN
PRODUCTION IN YEAST

Saccharomyces cerevisia,
Pichia pastoris,
stable expression

READ MORE

HTP

High Throughput protein expression screening

HIGH THROUGHPUT PROTEIN
EXPRESSION SCREENING

For difficult-to-express proteins, 1000+
expression conditions, small-scale, high
throughput screening

READ MORE

Incest cells

Protein production in Insect cells

PROTEIN PRODUCTION IN
INSECT CELLS / BACULOVIRUS

Sf9, Sf21, Hi5, glycosylated proteins,
protein purification, baculovirus
production

READ MORE

Mammalian

Protein production in Mammalian cells

PROTEIN PRODUCTION
IN MAMMALIAN CELLS

Transient expression, Recombinant antibody
production, SEC and IEX purifications,
Low endotoxin levels, CHO, HEK293, BHK

READ MORE

Stable cell line

Stable cell line development

STABLE CELL LINE
DEVELOPMENT

IP free systems, guaranteed yield, process
development, HEK293 and CHO stable
cell line

READ MORE

RAB

Recombinant antibody production services

RECOMBINANT MONOCLONAL
ANTIBODY PRODUCTION

Low cost production,
guaranteed yield,
HTP antibody variants

READ MORE

Large scale

Large scale protein production in fermenter and bioreactor

FERMENTATION / BIOREACTOR
LARGE - SCALE PROTEIN

1 to 4000L in each 5 systems,
Large scale purification services,
Process development services

READ MORE

Custom Protein Production
Testimonial

I recently chose ProteoGenix to produce a recombinant protein in HEK293 cells. The high productivity and strong activity of the protein obtained, probably due to proper glycosylation and folding, allowed us to generate exciting results in functional studies and reach new milestones in our research project. The quality of the service provided and the smooth and kind communication I had with the account manager in charge of our project were decisive in this success. I’m happy to recommend ProteoGenix for recombinant protein productions.

Dr Christian BRONNER,
PhD and principal scientist
at CNRS

Why Choose ProteoGenix

Process development experience
 More than 14 years of outstanding track record in the production of difficult-to-express proteins
5 protein expression systems
5 available protein expression systems and complete toolbox to overcome any particular protein expression challenges
One stop solution from gene to protein
One-stop solution from gene to protein, process development and large scale production. Consequences: faster service, increased success rate, less administrative work.
IP Free strains and cell lines
IP-free strains and cell lines
transient protein expression service
Very small to large scale productions with 1 to 4000 L batch
Transient expression and stable cell line
Productivity of up to 800mg/L in transient expression and 5g/L in stable expression

Fermentation facilities

Protein production in bacteria, yeast, insect, mammalian cells
Process development in bioreactors

Case Study:

Example of insect cells production of a Beta-Glucosidase

SERVICE PERFORMED

We were requested by a customer to produce a recombinant Beta-Glucosidase protein using a baculovirus expression system (Insect cells production). Our customer ordered a full Insect cells package including small scale expression and purification tests followed by a 1L pilot production and purification. We reached a yield of 370 mg/L and delivered 60mg after small scale expression. 1L pilot production was no longer useful and was therefore not charged to our customer. The service performed included:

MOLECULAR BIOLOGY

The cDNA coding for Beta-Glucosidase protein was chemically synthesized after sequence optimization for insect cells expression. It was then subcloned in a proprietary expression vector. A sequence coding for a 6His tag was added for further purification.

EXPRESSION TESTS

P1 VIRUS STOCK GENERATION

Gel recombinant protein expression results

Figure 1. Analysis of insect cell expression during P1 generation.
Left. Coomassie Blue Staining. Right. WB with anti-His antibody (ECL substrate) “-“. Un-transfected
control culture. 1 and 2 are transfections with 2 different Bacmid clones

Conclusion of P1 virus generation step

A protein band matching with Beta-Glucosidase protein molecular weight is observed by SDS-PAGE in native protein extracts. This result is confirmed by Western blot (Figure 1, green arrows). Recombinant bacmid clone No1 was used for expression tests.

VIRUS AMPLIFICATION AND EXPRESSION TESTS

Protein expression gel

Figure 2. Protein Expression Tests with P2 stock.
Coomassie Blue Staining. Left. Cell type 1.Right. Cell type 2. “-“. Negative control culture.

Conclusion of expression tests

Expression tests with P2 confirm that the target is present at high level in native protein extracts (Figure 2, green arrows). Optimal conditions for native expression: Figure 2, red arrow = loi

PURIFICATION TESTS

Recombinant protein purification test

Figure 3. Protein small-scale purification test. Coomassie Blue Staining.
Left. Purification profile. Right. Final QC of purification pool
IN. Input. FT. Flow through. W1-W3. Washing steps. E1-E9. Eluted fractions.

Conclusion of small-scale purification tests

Recombinant Beta-Glucosidase protein can be produced and purified in native conditions. The purity is ≥ 95%, and the production/purification yield is approximately 370mg/L. Baculovirus expression system was a perfect choice for this protein.

Which System to choose from

COMPARISON BETWEEN 5 EXPRESSION SYSTEMS PROVIDED BY PROTEOGENIX -
PROKARYOTIC VS EUKARYOTIC CELLS EXPRESSION SYSTEMS.

The table below gives an idea of the advantages of each five different heterologous protein expression systems for production of recombinant proteins from a general standpoint. Of course features can vary from one protein to another. The more arrows there are, the better it is.

Expression
system
TimeCostPTMLow Endotoxin levelsSimple process scale upSecretion
E. coli**************
B. subtills****************
Yeast (P. pastoris,
S. cerevisiae)
**************
Baculovirus/
Insect cells
**************
Mammalian cells******************

TIME DURATION AND PRICE

Expression systemTime durationPrice
E. coli3-6 weeksStartingfrom
1 650€
B. subtills4-6 weeks
Yeast (P. pastoris,
S. cerevisiae)
6-7 weeks
Baculovirus/
Insect cells
8-11 weeks
Mammalian cells7-9 weeks

Service Content
Small scale expression and purification evaluation with many expression conditions tested.
1L production and purification

Package codeTypeSystemsPrice
ATX-PTR4S014 systems
Expression package
E. coli / Yeast / Baculovirus /
Mammalian cells
Startingfrom
2 450€
ATX-PTR3S053 system
Expression package
B. subtills / Yeast / Baculovirus
ATX-PTR2S022 systems
Expression package
E. coli / Baculovirus

  The most commonly used system of recombinant protein purification is E. coli. Indeed, it is the simplest, fastest and most cost effective system for custom expression of a protein. Bacterial cultures grow much faster than insect cells, mammalian cells or yeast. It is therefore easier and quicker to test a large number of different conditions. More solutions are also available for testing and allow therefore a higher success rate in expressing recombinant proteins. Transient expression yields of recombinant protein are also generally much higher than in yeast, baculovirus / insect cells or mammalian cells. Production of recombinant proteins in E. coli (Escherichia Coli) is often a preferred solution proposed by ProteoGenix when the final application requires large quantities of proteins such as crystallography and nuclear magnetic resonance (NMR) and no Post-Translational Modifications (PTM) are required. However, the main drawbacks of E. coli system are the high amounts of endotoxins remaining in the final sample and the lack of Post-Translational modifications. It is possible to remove endotoxin but it isn't convenient at industrial stage whereas there is not much to be done to solve PTM problems apart from using a different expression system. With these five systems, we are able to meet the requirements of a large number protein synthesis order and we therefore have a success rate of over 95% in recombinant protein synthesis. We are constantly working on integrating new systems and solutions to provide the best level of service for anyone who needs to buy recombinant protein production services with high quality standards.

  If you have any doubt on which system to choose from, as a leading protein production manufacturer we guide you from gene to protein to define the optimal strategy considering your time, budget and protein features required.

Team, Capacity, Organization

  Our recombinant protein production laboratories are organized in several platforms and supporting labs to ensure the best quality of service, the highest purity of product, the lowest risk of contamination whatsoever and the best organization for a fast and reliable service.

  We have a platform for each of our five systems and a separate quality control lab, molecular biology lab, packing lab… Each platform is managed by experienced team leaders that ensure to delivers proteins that correspond to initial customer's specifications. After more than 1500 proteins produced today and teams specialized in each system, our expertise has no equivalent for each of these systems.

Custom recombinant protein
expression applications

  ProteoGenix recombinant proteins are used in wide spectrum of applications and our customers usually involved in Life sciences, include Pharma companies, Diagnostics, Biotech, Agri-Food Industry, Crop science, New energies, Cosmetics, National Research Laboratories, etc.

  Below is a non-exhaustive list of applications:

  • Protein involved in diagnostic assays for calibration, positive controls or capture components
  • Production of recombinant antibody variants for therapeutic antibodies humanization
  • Production of food enzymes involved in food production processes or quality controls
  • Recombinant protein antigen for the production of polyclonal or monoclonal antibodies (antigen preparation)
  • Functional and activity studies
  • Crystallography
  • Protein interaction studies
  • Patent issue investigations
  • Mutant engineering studies for drug efficacy improvements in drug discovery