With more than 15 years of extensive experience in custom protein expression, and over 1500 proteins successfully produced in 5 systems, ProteoGenix is a leader in difficult-to-express recombinant proteins and antibodies productions.

Custom Protein Production

Dr Christian BRONNER,
PhD and principal
scientist at CNRS

” I recently chose ProteoGenix to produce a recombinant protein in HEK293 cells. The high productivity and strong activity of the protein obtained, probably due to proper glycosylation and folding, allowed us to generate exciting results in functional studies and reach new milestones in our research project. The quality of the service provided, and the smooth and kind communication I had with the account manager in charge of our project, were paramount to this success. I’m happy to recommend ProteoGenix for recombinant protein productions. “

Fermentation facilities

Protein production in bacteria, yeast, insect, mammalian cells
Process development in bioreactors

Case Study:

Claudiximab protein expression

A customer requested the recombinant expression of Claudiximab. He ordered a protein expression package including:

  • Gene synthesis with codon optimization
  • Subcloning in an expression vector
  • Small-scale protein expression and purification

Gene Synthesis And Subcloning

The genes coding for the Heavy chain (HC) and the light chain (LC) of the therapeutic antibody Claudiximab were chemically synthesized with optimization for protein expression in mammalian cells. The genes were cloned in ProteoGenix’s proprietary mammalian cells expression vector pTXs1.

Protein Expression

recombinant protein expression purification

Figure 1. Purification profile of Claudiximab. Reduced PAGE with Coomassie blue staining.
IN. Input sample. FT. Flow through. W. Wash step. E1-E11. Eluted fractions.

protein production QC

Figure 2. Final QC of purified Claudiximab. Coomassie blue staining.
A. Non-reduced PAGE. B. Reduced PAGE

Production Yield Purity
Claudiximab 1281mg/L ≥95%

Which System choose for your Protein Production?

Comparison Between 5 Expression Systems Provided By
Proteogenix –
Prokaryotic Vs Eukaryotic Cells Expression Systems

Expression system Time Cost PTM Low Endotoxin levels Simple process scale up Secretion
E. coli **** **** * * *** *
B. subtilis *** *** * **** *** **
Yeast (P. pastoris, S. cerevisiae) * *** ** **** ** **
Baculovirus/ Insect cells * ** *** **** * ***
Mammalian cells *** * **** **** ** ****
Expression system Time duration Price
E. coli 3-6 weeks Starting from 1650€
B. subtilis 4-6 weeks Starting from 1650€
Yeast (P. pastoris, S. cerevisiae) 6-7 weeks Starting from 1650€
Baculovirus/ Insect cells 8-11 weeks Starting from 1650€
Mammalian cells 7-9 weeks Starting from 1650€

Service Content
Small scale expression and purification evaluation with many expression conditions tested.
1L production and purification

Package code Type Systems Price
ATX-PTR4S01 4 systems Expression package E. coli / Yeast / Baculovirus / Mammalian cells Starting from 2450€
ATX-PTR3S05 3 system Expression package B. subtilis/ Yeast / Baculovirus Starting from 2450€
ATX-PTR2S02 2 systems Expression package E. coli / Baculovirus Starting from 2450€

The most commonly used system of recombinant protein purification is E. coli. Indeed, it is the simplest, fastest and most cost effective system for custom expression of a protein. Bacterial cultures grow much faster than insect or mammalian cells or yeast. It is therefore easier and quicker to test a large number of different conditions. More solutions are also available for testing and therefore allow a higher success rate in expressing recombinant proteins. Transient expression yields of recombinant protein are also generally much higher than in yeast, baculovirus / insect cells or mammalian cells. Production of recombinant proteins in E. coli (Escherichia Coli) is often the preferred solution proposed by ProteoGenix when the final application requires large quantities of proteins such as crystallography and nuclear magnetic resonance (NMR) and no Post-Translational Modifications (PTM) are required. However, the main drawbacks of E. coli system are the high amounts of endotoxins remaining in the final sample and the lack of Post-Translational modifications. It is possible to remove endotoxins but it isn’t convenient at an industrial stage when there is not much to be done to solve PTM problems apart from using a different expression system. With these five systems, we are able to meet the requirements of a large number protein synthesis orders and we therefore have a success rate of over 95% in recombinant protein synthesis. We are constantly working on integrating new systems and solutions to provide the best level of service for anyone who needs to buy high quality recombinant protein production services.

Team, Capacity, Organization

If you have any doubts about which system to choose from, we, as a leading protein production manufacturer, can guide you from gene to protein and help you define an optimal strategy while taking into account your time constraints, budget and required protein features..

Our recombinant protein expression laboratories are organized across several platforms and supporting labs to ensure the best quality of service, the highest product purity, the lowest risk of contamination and the best organization for a fast and reliable service.

We have a platform for each of our five systems and a separate quality control lab, molecular biology lab, packing lab… Each platform is managed by experienced team leaders that ensure they deliver proteins corresponding to initial customer specifications. With more than 1500 proteins produced to date, and specialized teams for each system, our expertise is unrivalled.

Custom recombinant protein
expression applications

ProteoGenix recombinant proteins are used in a wide spectrum of applications and our customers are usually involved in Life sciences, including Pharma companies, Diagnostics, Biotech, the Agri-Food Industry, Crop science, New energies, Cosmetics, National Research Laboratories, etc..

Below is a non-exhaustive list of applications:

  • Protein involved in diagnostic assays for calibration, positive controls or capture components
  • Production of recombinant antibody variants for therapeutic antibodies humanization
  • Production of food enzymes involved in food production processes or quality controls
  • Recombinant protein antigen for the production of polyclonal or monoclonal antibodies (antigen preparation)
  • Functional and activity studies
  • Crystallography
  • Protein interaction studies
  • Patent issue investigations
  • Mutant engineering studies for drug efficacy improvements in drug discovery