Frustrated by the antibody yield of your hybridomas?
40 mg/L in medium with serum,
20 mg/L in serum/animal free medium.
Get your antibodies without any animal contaminant.
Get endotoxin free antibodies (<1EU/mg).
We have all the technologies to adapt your production to your needs.
We propose recombinant antibody production if your hybridoma cell line is unstable.
Choose ProteoGenix from hybridoma generation to hybridoma cell culture.
“One of our hybridomas was unstable, difficult to grow and with a poor antibody yield. ProteoGenix was able to rescue it and produce large scale batches of purified antibodies of consistent quality. We appreciated ProteoGenix’s hard work and commitment to successfully deliver this difficult project which was of critical importance for us.”
“ProteoGenix is a reliable partner for supplying both in vitro and in vivo mAbs. I appreciated the collaborative and prompt problem solving attitude of the management, which definitely added value to our deal.”
Synonyms: Animal-product free (APF), animal component-free (ACF), animal-derived component-free (ADCF), non-animal origin medium
To order hybridoma cell culture, download this form and send it to us by email at email@example.com.
To order ascites production, click here to download the file and send it by email to firstname.lastname@example.org.
Hybridoma cell culture is an in vitro alternative of ascites production. This technique does not require the use of animals and can produce antibodies free of contaminants (if produced in animal free medium).
Hybridoma cell culture consists in growing hybridoma cells and harvesting the culture supernatant (containing the antibodies). Briefly, the hybridoma cell culture occurs in two phases:
The first one is optimal for cell growing. This step is characterized by a higher presence of serum compared to the second step.
The second step is more optimal for antibody production as it contains less or no animal component depending on the option chosen (serum free or animal free culture medium).
It exists two different ways to adapt the hybridoma cell line to the antibody production medium:
The direct adaptation: in this technique, the hybridoma cells are directly adapted from serum-containing medium to serum free medium.
The sequential adaptation: the sequential adaptation is based on a progressive depletion of the amount of serum in the culture medium. It is generally used if the direct adaptation does not work.
The expected yield of hybridoma cell culture is around 10-50mg/L. If higher yields or high amount of antibodies are necessary, we would suggest using our recombinant antibody production service. Any doubt about the most relevant antibody production technique for your project? Check the following table or contact our dedicated account manager.
Ascites production requires an available hybridoma cell line. This technique is an in vivo antibody production method based on the injection of hybridoma cells into the abdominal cavity of a rodent to produce ascites fluid. This fluid contains a high concentration of antibodies which can generally not be obtained by classical in vitro methods.
Despite these advantages, we would never advice our customers to order this technique except if there is no other alternative. Alteration of the antibody properties during the purification step could be such an exception. In this case, ascites antibody production could be necessary as the high concentration of the antibody produced allows the use of the ascitic fluid as is (without purification step).
To order ascites production, click here to download the file and send it by email to email@example.com
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