Your antibody production yields are not satisfying? You cannot reach sufficient purity for your application? With up to 40 mg/L antibody yield guaranteed and the possibility to get high purity and low endotoxin antibodies, our hybridoma development platform and cell culture service will overcome the most difficult challenges.

Testimonial for ascites and hybridoma cell culture antibody production

Senior Scientist
at Pfizer

“One of our hybridomas was unstable, difficult to grow and with a poor antibody yield. ProteoGenix was able to rescue it and produce large scale batches of purified antibodies of consistent quality. We appreciated ProteoGenix’s hard work and commitment to successfully deliver this difficult project which was of critical importance for us.”

Manager at Agritest

“ProteoGenix is a reliable partner for supplying both in vitro and in vivo mAbs. I appreciated the collaborative and prompt problem solving attitude of the management, which definitely added value to our deal.”

Production Method Production Method Pros Cons
Mouse Ascites Mouse Ascites
  • Best and easiest for hybridomas
  • Stable antibody yields + very high antibody concentrations → purification easier + no further concentration procedures required
  • Animal use
  • Purified antibody can contain residual mouse contaminants such as IgG
Hybridoma cell culture Medium With serum
  • Animal protection: no animal use, injection, sacrifice → more ethical
  • Almost same price + lead time as ascites production
  • Lower antibody concentrations than ascites, which requires concentrating steps that might change antibody affinity or denature antibody
  • Purified antibody can contain residual animal contaminants such as IgG
Serum-free Medium
  • Animal protection: no animal use, injection, sacrifice → more ethical
  • Better defined and reproducible medium formulations than serum medium → higher antibody yields + more consistent productions
  • Elimination of a source of possible infectious agents (viruses, prions, bacteria)
  • Reduction of purified antibody contamination by culture residues such as animal IgG → reduced risk of interferences in experiments
  • Much cheaper than cultures with ultra low IgG serum
  • Lower antibody concentrations than ascites, which requires concentrating steps that might change antibody affinity or denature antibody
  • Potential persistence of residual animal contaminants in purified antibody
  • More expensive + may take more time than serum/ascites production
Animal-free Medium
  • Same as serum-free but with 100% elimination of other animal contaminants
  • No risk of purified antibody contamination by animal components such as proteins → risk of interferences in experiments dramatically reduced
  • Best if purified antibody to be used for in vivo experiments

Synonyms: Animal-product free (APF), animal component-free (ACF), animal-derived component-free (ADCF), non-animal origin medium

  • Lower antibody concentrations than ascites, which requires concentrating steps that might change antibody affinity or denature antibody
  • More expensive + may take more time than serum/serum-free/ascites production
Ascites Hybridoma cell culture Recombinant antibody production
Concentration 1-3mg/mL 10-50mg/L From mg to g/L
Volume 2-5 mL/animal From mL to L range From L to hundreds of L

Ascites antibody production principle

Ascites production requires an available hybridoma cell line. This technique is an in vivo antibody production method based on the injection of hybridoma cells into the abdominal cavity of a rodent to produce ascites fluid. This fluid contains a high concentration of antibodies which can generally not be obtained by classical in vitro methods.

Despite these advantages, we would never advice our customers to order this technique except if there is no other alternative. Alteration of the antibody properties during the purification step could be such an exception. In this case, ascites antibody production could be necessary as the high concentration of the antibody produced allows the use of the ascitic fluid as is (without purification step).

To order ascites production, click here to download the file and send it by email to contact@proteogenix.fr