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Ascites production and purification

 

As a monoclonal and polyclonal antibody expert, ProteoGenix offers all related services such as ascites production and purification from mice. We can produce antibodies on a small or large scales in ascites, even though we recommend producing culture supernatants for large scale production. You can provide your own hybridomas or let us develop new hybridomas to use for your Ig production request.

To order ascites production, click here to download the file and send it by email to contact@proteogenix.fr

Production in culture supernatant

 

As part of our custom antibody services, we produce antibodies from culture supernatants either from hybridomas developed by ourselves or hybridomas provided by our customers. We can produce milligrams to grams of purified antibody using serum, serum-free or animal-free culture medium depending on your needs. We have various technologies available for production, such as flasks, roller bottles, hollow fibers, wave bags and bioreactors.
ProteoGenix can also check your antibodies for mycoplasma, identify the isotype, define the best production method, make pilot production runs, or produce master and working cell banks.

Download this form and send it to us by email at contact@proteogenix.fr. We will reply with your quote in 24 hours. You can then send your PO to order any antibody production and purification in cell culture.

Testimonial for Ascites & Culture Supernatant Production

“ProteoGenix is a reliable partner for supplying either in vitro
and in vivo mAbs. I appreciated the collaborative and prompt
problem solving attitude of the management, which definitely
added value to our deal."

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Lilia Formica, R&D Manager at Agritest


Pros and cons of the different antibody production methods offered by ProteoGenix

 

Production Method Pros Cons
Mouse Ascites
  • Best and easiest for hybridomas
  • Stable antibody yields + very high antibody concentrations
    → purification easier + no further concentration procedures required
  • Animal use
  • Purified antibody can contain residual mouse contaminants such as IgG
Cell Culture Supernatant Medium With serum
  • Animal protection: no animal use, injection, sacrifice
    → more ethical
  • Almost same price + lead time as ascites production
  • Low antibody concentrations than ascites, which requires concentrating steps that might change antibody affinity or denature antibody
  • Purified antibody can contain residual animal contaminants such as IgG
Serum-free Medium
  • Animal protection: no animal use, injection, sacrifice
    → more ethical
  • Better defined and reproducible medium formulations than serum medium
    → higher antibody yields + more consistent productions
  • Elimination of a source of possible infectious agents (viruses, prions, bacteria)
  • Reduction of purified antibody contamination by culture residues such as animal IgG
    → reduced risk of interferences in experiments
  • Much cheaper than cultures with ultra low IgG serum
  • Lower antibody concentrations than ascites, which requires concentrating steps that might change antibody affinity or denature antibody
  • Potential persistence of residual animal contaminants in purified antibody
  • More expensive + may take more time than serum/ascites production
Animal-free Medium

 

  • Same as serum-free but with 100% elimination of other animal contaminants
  • No risk of purified antibody contamination by animal components such as proteins
    → risk of interferences in experiments dramatically reduced
  • Best if purified antibody to be used for in vivo experiments

 

Synonyms: Animal-product free (APF), animal component-free (ACF), animal-derived component-free (ADCF), non-animal origin medium

  • Lower antibody concentrations than ascites, which requires concentrating steps that might change antibody affinity or denature antibody
  • More expensive + may take more time than serum/serum-free/ascites production