Ascites and hybridoma cell culture antibody production

Antibody production form

Your antibody production yields are not satisfying? You cannot reach sufficient purity for your application? With up to 40mg/L antibody yield guaranteed and the possibility to get high purity and low endotoxin antibodies, our hybridoma cell culture service will overcome the most difficult challenges.

Why choose ProteoGenix for ascites or
hybridoma cell culture antibody production?

Hybridoma cell culture yield guarantee
Yield guaranteed

40 mg/L in medium with serum,
20 mg/L in serum/animal free medium.

high purity antibodies
High purity antibodies (>95%)

Get your antibodies without any animal contaminant.

endotoxin free antibodies
Endotoxin free

Get endotoxin free antibodies (<1EU/mg).

large scale hybridoma cell culture
From mg to g antibody production

We have all the technologies to adapt your production to your needs.

Secured hybridoma cell line
Secure your hybridoma cell line

We propose recombinant antibody production if your hybridoma cell line is unstable.

From antigen design to hybridoma cell culture
From antigen design to production

Testimonial for ascites and hybridoma cell culture antibody production

“ProteoGenix is a reliable partner for supplying both in vitro and in vivo mAbs. I appreciated the collaborative and prompt problem solving attitude of the management, which definitely added value to our deal.”

Satisfied hybridoma cell culture customer
Lilia Formica, R&D
Manager at Agritest

“ProteoGenix is a reliable partner for supplying both in vitro and in vivo mAbs. I appreciated the collaborative and prompt problem solving attitude of the management, which definitely added value to our deal.”

Satisfied hybridoma cell culture customer
Lilia Formica, R&D
Manager at Agritest

Ascites vs. hybridoma cell
culture antibody production

As affinity maturation is fairly complex, our experts define the most relevant strategy after a preliminary study. This is the best way to guarantee our customers a safe investment and to include unique result guarantees to your project. This demonstrates our commitment to the success of your antibody affinity maturation.

Production MethodProsCons
Mouse Ascites
  • Best and easiest for hybridomas
  • Stable antibody yields + very high antibody concentrations
    → purification easier + no further concentration procedures required
  • Animal use
  • Purified antibody can contain residual mouse contaminants such as IgG
Hybridoma cell cultureMedium With serum
  • Animal protection: no animal use, injection, sacrifice
    → more ethical
  • Almost same price + lead time as ascites production
  • Low antibody concentrations than ascites, which requires concentrating steps that might change antibody affinity or denature antibody
  • Purified antibody can contain residual animal contaminants such as IgG
Serum-free Medium
  • Animal protection: no animal use, injection, sacrifice
    → more ethical
  • Better defined and reproducible medium formulations than serum medium
    → higher antibody yields + more consistent productions
  • Elimination of a source of possible infectious agents (viruses, prions, bacteria)
  • Reduction of purified antibody contamination by culture residues such as animal IgG
    → reduced risk of interferences in experiments
  • Much cheaper than cultures with ultra low IgG serum
  • Lower antibody concentrations than ascites, which requires concentrating steps that might change antibody affinity or denature antibody
  • Potential persistence of residual animal contaminants in purified antibody
  • More expensive + may take more time than serum/ascites production
Animal-free Medium
  • Same as serum-free but with 100% elimination of other animal contaminants
  • No risk of purified antibody contamination by animal components such as proteins
    → risk of interferences in experiments dramatically reduced
  • Best if purified antibody to be used for in vivo experiments

Synonyms: Animal-product free (APF), animal component-free (ACF), animal-derived component-free (ADCF), non-animal origin medium

  • Lower antibody concentrations than ascites, which requires concentrating steps that might change antibody affinity or denature antibody
  • More expensive + may take more time than serum/serum-free/ascites production

Hybridoma cell culture principle

Hybridoma cell culture is an in vitro alternative of ascites production. This technique does not require the use of animals and can produce antibodies free of contaminants (if produced in animal free medium).

Hybridoma cell culture consists in growing hybridoma cells and harvesting the culture supernatant (containing the antibodies). Briefly, the hybridoma cell culture occurs in two phases:

  1. The first one is optimal for cell growing. This step is characterized by a higher presence of serum compared to the second step.

  2. The second step is more optimal for antibody production as it contains less or no animal component depending on the option chosen (serum free or animal free culture medium).

It exists two different ways to adapt the hybridoma cell line to the antibody production medium:

  1. The direct adaptation: in this technique, the hybridoma cells are directly adapted from serum-containing medium to serum free medium.

  2. The sequential adaptation: the sequential adaptation is based on a progressive depletion of the amount of serum in the culture medium. It is generally used if the direct adaptation does not work.

The expected yield of hybridoma cell culture is around 10-50mg/L. If higher yields or high amount of antibodies are necessary, we would suggest using our recombinant antibody production service. Any doubt about the most relevant antibody production technique for your project? Check the following table or contact our dedicated account manager.

AscitesHybridoma cell cultureRecombinant antibody production
Concentration1-3mg/mL10-50mg/LFrom mg to g/L
Volume2-5 mL/animalFrom mL to L rangeFrom L to hundreds of L

Ascites antibody production principle

Ascites production requires an available hybridoma cell line. This technique is an in vivo antibody production method based on the injection of hybridoma cells into the abdominal cavity of a rodent to produce ascites fluid. This fluid contains a high concentration of antibodies which can generally not be obtained by classical in vitro methods.

Despite these advantages, we would never advice our customers to order this technique except if there is no other alternative. Alteration of the antibody properties during the purification step could be such an exception. In this case, ascites antibody production could be necessary as the high concentration of the antibody produced allows the use of the serum as is (without purification step).

To order ascites production, click here to download the file and send it by email to contact@proteogenix.fr