ProteoGenix has more than 13 years of experience in recombinant protein production in E. coli and is therefore a major player in this industry for the production of mg to gram levels of protein in flasks or fermentors. E. coli is the most widely used system for recombinant protein expression as is it possible to vary many conditions at every step of the production process, and it allows a high success rate for protein expression compared to other systems.

 

Depending on our customer’s budget we can carry on a large number of tests (several thousands) thanks to our high throughput platform in just a few weeks or only a few (more than 10 though) in our standard package described below.



Standard Escherichia coli Protein Expression package:

  • Project study
  • Bacteria strains transformation
  • Expression evaluation under different conditions (strain, temperature, time, inductor concentration…)
  • SDS/PAGE analysis and WB if necessary
  • Purification tests
  • SDS/PAGE analysis and WB if necessary
  • 1L expression and purification scale up
  • SDS/PAGE analysis and WB if necessary
  • Final detailed report
  • Purified protein
  • 3 to 5 weeks

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