Antibody phage display services

Phage Display form

    Discover LiAb-SFCOVID-19TM the first antibody library designed to fight the COVID-19 pandemic

    Accelerate your development program with ProteoGenix antibody phage display services and get your purified recombinant antibodies in less than 2 months! Our libraries are made of various monoclonal antibody formats including Fab, scFv or VHH and species! Thanks to our highly diversified human libraries, humanization is not necessary for your therapeutic antibody development and you benefit from our strong guarantees!

    Why choose ProteoGenix' antibody
    phage display services?

    Guaranteed phage display services
    Binders guaranteed

    We guarantee AT LEAST 3 unique binders against your antigen!

    IP free phage display antibody production
    IP free

    You get the full ownership of the antibody sequence generated!

    Antibody phage display libraries
    Very high diversity antibody phage display libraries

    Our premium libraries contain at least 1010 different variants!

    Phage display antibody formats
    scFv, Fab or VHH formats available

    The variety of our libraries allows us to propose a wide range of formats to fit a wide range of applications.

    Antibody phage display immune library
    Wide variety of species available

    Mouse, sheep, llama, rabbit monoclonal antibodies etc.As well as our naïve libraries, you can also choose to develop immune libraries from a species of your choice without limitation.

    Fast monoclonal antibody generation
    Save time!

    From antigen to antibody in less than 7 weeks!

    Animal free monoclonal antibody production
    No animal use

    Naïve library screening does not require any animal use.

    antibody library screening
    Buy your library

    Buy our naïve libraries or your own immune libraries!

    Naïve or immune antibody phage display libraries?

    Naïve libraries Immune libraries
    Species Human, rabbit, camelids (camel, llama, alpaca) No limitation except humans
    Formats Fab, scFv, VHH Fab, scFv, VHH
    Number of binders ++ +++
    Affinity of binders ++ +++
    Potential immunogenicity issues No Yes
    Animal use No Yes
    Timelines 6-7 weeks 14-19 weeks + 6-7 weeks
    Price + ++

     

    Interested in our naïve library screening service or in constructing an immune library ? Please feel free to contact our dedicated account manager.

    Find your perfect antibody phage display library!

    Library Format Species Size (clones)

    NEW!

    LiAb-SFCOVID-19TM

    scFv Human – Donors that recovered from COVID-19 – Ideal for the identification of SARS-CoV-2 binders 1.19 X 1010
    LiAb-SFMAXTM scFv & Fab Human – 5 different ethnic groups – 368 donors for maximized diversity 5.37 X 1010
    LiAb-SFaTM scFv Human 1.5 X 109
    LiAb-FabTM Fab Human 2.00 X 1010

    NEW!

    LiAb-VHHMAXTM

    VHH Camel, llama, alpaca – 57 animals – Ideal for nanobody generation 1.51 X 1010
    LiAb-SFRabTM scFv & Fab Rabbit – 4 different breeds for maximized diversity 1.09 X 1010
    LiPep-12TM Peptide 12-mer / 1.00 X 109
    LiPep-7TM Peptide 7-mer / 1.00 X 109

     

    ProteoGenix developed a large choice of proprietary phage display libraries that you can adapt to all your projects. Our goal is to fulfill all your unique requirements and to always push the boundaries of innovation by creating new and better phage display libraries. Whether you need a fully human antibody or a nanobody for therapeutic applications or a high-affinity rabbit antibody for diagnostic applications, our libraries will help you overcome your most difficult challenges. Feel free to contact our account manager for more information.

    Our antibody phage display service process

    Antibody phage display library construction
    Antigen procurement or design and production
    • Peptide/small molecules: conjugation to carriers
    • Protein production including gene synthesis
    • Cell overexpressing target protein

    Immune library

    Immune library

    Naive library

    Antibody phage display library construction
    Immune library construction
    • PBMC isolation
    • RNA extraction and cDNA synthesis
    • VH and VL PCR amplification
    • Library construction and QC
    Antibody phage display screening
    Library screening and biopanning
    • Screening of naive or immune library against antigen
    • 4-6 rounds of biopanning
    Single phage screening
    ELISA screening of single phage binders
    • ELISA screening and validation until identification of at least 3-10 different binders
    Antibody sequencing
    DNA extraction & antibody sequencing

    Our antibody phage display service content

    Step Content Timelines Deliverables
    Library construction (only for immune libraries)
    • Animal immunization and titer tests
    • Cell isolation (spleen, bone marrow, PBMC)
    • RNA extraction
    • cDNA synthesis
    • VH and VL PCR amplification
    • cDNA assembly
    • Phagemid synthesis and cloning
    • Library QC
    14-19 weeks
    • Progress report
    Library screening and biopanning
    • Screening of the naïve or immune library
    • 4-6 rounds of biopanning
    6-7 weeks
    • Progress report
    ELISA screening
    • Screening and validation by ELISA until at least 3-10 different binders are identified
    • Progress report
    DNA extraction and antibody sequencing
    • Phage DNA extraction and antibody sequencing
    • Detailed report
    • DNA sequence of the best binder
      Full ownership of the sequences
    Options available:
    • Additional screening of single phage clones by ELISA against a specific protein/peptide/small molecule/cell
    • Additional screening of single phage clones by WB
    • Additional screening of single phage binders by flow cytometry on cells
    • Assessment of the affinity of single phage binders
    • Panning against cells overexpressing antigen of interest with library depletion against control cell

    Satisfied antibody phage display customer testimonial

    “All three of the antibodies you delivered were reformatted into IgGs and worked well. Interestingly two were d1 binders, and one was a d1/d2 binder when we domain mapped them here. They were good “protein X” blocking antibodies. But none were “protein Y” blockers. They express reasonably well. Overall we are quite happy with them.”

    Satisfied antibody phage display customer

    Senior scientist at aTyr Pharma, USA

    Case study: antibody generation against a small molecule by phage display

    Project requirements

    One of our customers requested a human antibody against a small molecule.
    The small molecule was conjugated to different carriers to achieve optimal coating.
    Biopanning was performed using our human naive library of high diversity.
    A total of 192 single phage binders were screened and validated by ELISA.
    10 antibody sequences were required.

    Biopanning results

    Rounds Phage input (pfu) Phage output (pfu)
    Panning with antigen coating Panning with no coating (negative control)
    1 2.0×1012 8.9×106 3.4×106
    4 1.0×1012 4.0×107 2.5×106

    There is an obvious enrichment in anti-antigen binders even if it is difficult to remove the binders to carriers (known phenomenon).

    Polyclonal phage ELISA

    Phage quantity (pfu/well) Round 3 Round 4
    Antigen Control Antigen Control
    6×1012 3.035 2.584 3.630 3.451
    2×1012 2.798 1.709 3.308 3.007
    6.7X1011 1.508 1.069 3.028 2.128
    2.2×1011 0.5666 0.5024 2.976 1.885
    7.4×1010 0.1712 0.1932 2.581 1.704
    2.5×1010 0.08020 0.09040 2.033 1.317
    8.2×109 0.06090 0.09140 1.931 1.054
    2.7×109 0.01620 0.01740 0.7235 0.7526
    9×108 0.01290 0.01460 0.2341 0.2432

    Although there are many unspecific binders (known phenomenon), an obvious enrichment in anti-antigen binders is observed (data in bold).

    Monoclonal phage ELISA of the 11 unique antibodies identified (unique means unique sequence)

    Clones Ag-carrier 1 Ag-carrier 2 Ag-carrier 3 No coating Carrier 1 Carrier 2 Carrier 3
    A6 4.698 4.743 4.466 0.118 0.100 0.092 0.117
    A9 4.769 4.766 4.420 0.100 0.092 0.078 0.074
    B1 4.716 4.804 4.452 0.353 0.584 0.422 0.187
    B2 4.494 4.482 4.874 0.081 0.122 0.109 0.100
    D1 4.862 3.989 4.540 0.170 0.488 0.313 0.123
    D8 4.862 4.090 4.486 0.057 0.106 0.102 0.059
    F4 4.214 3.352 5.306 0.051 0.110 0.123 0.059
    H2 4.797 4.537 4.588 0.084 0.213 0.188 0.112
    H11 4.722 4.604 5.049 0.103 0.218 0.262 0.138
    1A11 4.407 4.528 4.562 0.054 0.086 0.115 0.079
    1G1 4.956 4.381 5.218 0.076 0.114 0.138 0.077

    We generated 11 unique clones that specifically and strongly bind to the small molecule antigen.
    Our customer decided to express these 11 clones as recombinant antibodies and to order the screening of 192 additional single phages, which resulted in the identification of 7 new unique sequences.

    Learn more about our capabilities of antibody phage display services. Discover our complete report by clicking on the “DOWNLOAD PDF REPORT” button.

    What is antibody phage display?

    The phage display technology involves the determination of interaction partners of a protein (protein-protein, protein-DNA or protein-peptide interactions), which would be used as the “bait” for phages expressing peptides, proteins or antibodies coded by a huge DNA library (consisting of all coding sequences of a given organism). The function of that protein may therefore be determined.

    Phage display cycle: phage display library, panning/biopanning and validation by ELISA

    The phage display technique uses the filamentous phage, a virus that lives on E. coli. This method has proven to be a powerful technique for the interrogation of libraries containing billions of different proteins or peptides.
    The application of phage display on large antibody libraries has been a real success with regard to the isolation of monoclonal antibodies which are highly specific against an antigen.
    These new methods, using libraries composed of billions of antibodies expressed on phages, offer highly valuable alternatives that have proven to be as effective as traditional methods in the generation of billions of components and their quick selection to identify the most interesting candidates for your research.

    As such, phage display is a useful and state-of-the-art tool widely used in therapeutic antibody discovery.

    Advantages of antibody phage display

    The major advantage of phage display lies in the simplified structure, stability and therefore reliability of phages, making it possible to be applied to various surfaces but also to in vivo applications.
    The main characteristic of the phage display technique is the physical coupling between genotype and phenotype (phage will « display » the protein on its outside while containing the gene for the protein inside). This requires the artificial introduction of encoding DNAs into phages, explaining the large diversity of phage-based antibody libraries. The engineered antibody fragments, which are generated by phage display at ProteoGenix, are single chain variable fragment format (scFv), Fab-based antibody format presentation and single-domain antibodies (VHH).

    M13 bacteriophage in antibody phage display

    All ProteoGenix antibody phage display libraries are made of the M13 filamentous phage, a virus that infects the bacterium E. coli. This bacteriophage is the most commonly used for phage display and will be appropriate for you research.

    M13 is a lysogenic filamentous phage, comprised of a circular and single-stranded DNA encased in a thin flexible tube made up of about 2700 copies of a single protein called pVIII, the major coat protein, and about 5 copies of pIII, the minor coat protein, present at the ends of the tube. Infection with M13 plasmids is not lethal for bacteria and they are used for many recombinant DNA processes.