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Are you seeking a highly specific, application-ready antibody? With ProteoGenix’s dual-platform approach to custom rabbit antibody discovery, you’ll access the exact antibody you need, tailored to any antigen.
Select our “Application Guaranteed” packages to ensure your antibody works seamlessly in your desired application (ELISA, sandwich ELISA, Western Blot, flow cytometry, immunofluorescence, immunoprecipitation, immunohistochemistry).
From non-immunogenic to toxic antigens, our platforms can successfully generate the antibody you need.
Receive your antibodies in as few as four weeks.
Using our proprietary naïve library, we can develop antibodies without rabbit immunization.
For rabbit antibody development using phage display, it’s essential to consider two scenarios:
Naïve SFRab™ Library : Ideal for projects needing rapid turnaround or when immunization isn’t feasible (e.g., toxic, non-immunogenic, or novel antigens). With this fully animal-free library, screening is swift and straightforward.
Immune Library : When immunization is possible, an immune library becomes invaluable. While immune libraries may have reduced diversity, they provide a highly specific selection of antibodies against a particular antigen. The antibodies generated through this method often demonstrate exceptional affinity and developability, thanks to in vivo affinity maturation.
Single B cell screening is the method of choice for generating rabbit antibodies with high affinity and specificity. This technology isolates B cells from an immunized rabbit based on affinity to the target antigen, resulting in natural VH/VL pairing and optimized developability.
At ProteoGenix, we consider this approach ideal for diagnostic antibody development against known targets. That’s why our diagnostic antibody services with single B cell screening come with an “Application Guaranteed” performance warranty.
The following table summarizes the strengths and applications of each rabbit monoclonal antibody development platform. This overview helps you choose the optimal strategy for your project, though we recommend further consideration of your target antigen characteristics and final application.
| Criteria | Single B Cell Screening | Phage Display – Naïve Library | Phage Display – Immune Library |
|---|---|---|---|
| Affinity | +++ | + | +++ |
| Diversity | ++ | +++ | ++ |
| Specificity | +++ | + | +++ |
| Developability | +++ | + | ++ |
| Use of Rabbits | Yes | No | Yes |
| Turnaround time | ++ | + (fastest) | ++++ |
| Budget | ++ | + (less expensive) | +++ |
| Applications | For diagnostics and therapeutics - Ideal for diagnostics with “Application Guaranteed” package | Suitable for non-immunogenic/toxic targets or short timelines | For diagnostics and therapeutics - Optimized for therapeutic use to discover a large number of binders |
With multiple rabbit antibody development platforms, ProteoGenix addresses diverse project needs. For a closer look at our approach to custom rabbit antibody development via phage display or single B cell screening, download our two case studies below. These reports include:
Project description
Strategy used for antibody generation
Complete set of project results
At ProteoGenix, our team of experts and advanced technologies ensure the best possible approach to your custom rabbit antibody project. Every project begins with a consultation with one of our specialists, where you can provide your project specifications and any target-specific details. We’ll propose the best antibody generation strategy and provide a detailed quote. Once approved, simply submit your antigen sequence, and your project begins!
| Step | Details | Timeline | Deliverables |
|---|---|---|---|
| Rabbit Immunization | Antigen validation by SDS-Page; immunization with our RocketAbs protocol; bleedings, serum titer tests | 29-43 days | |
| Single B-cell Sorting & Screening | PBMCs and spleen lymphocyte isolation, single B-cell sorting by FACS, B-cell cultivation, screening | 2-3 weeks | Up to 30 supernatant samples |
| Recombinant Rabbit Antibody Production | Sequence top clones, transient expression, ELISA screening | 2 weeks | Up to 10 supernatant samples, report, amino acid sequences of top clones |
| Step | Naïve Library | Immune Library | Timeline | Deliverables |
|---|---|---|---|---|
| Rabbit Immunization | x | 29-43 days | ||
| PBMC Isolation | x | 1 week | ||
| PCR Amplification | x | 1 week | ||
| Library Construction | x | 1-2 weeks | Final report Library kept by ProteoGenix for further screening service or transfer to customer with full intellectual property | |
| Library Screening (4-6 rounds of library panning) | x | x | ||
| Screening & validation by ELISA | x | x | ||
| Phage DNA extraction + antibody sequencing | x | x | 4-5 weeks from library screening to antibody sequencing | Final report DNA sequence of the best binder Full ownership of the sequences |
In this video, Justine Warner, Business Manager for Monoclonal Antibodies at ProteoGenix, shares a personal story from the front lines of biotech innovation.
She takes you behind the scenes of a unique CAR-T project—highlighting the challenges, the journey, and the original solutions developed to target a rare pMHC complex.
