Package details

 
  • Antigen design (design of the most appropriate fragment to be produced)
  • Two gene syntheses (each gene optimized for a specific expression system) and 2 subclonings
  • Expression tests in two systems taken from E. coli, B. subtilis, P. pastoris, insect cells, and mammalian cells. To be determined by ProteoGenix.
  • Production of the antigen in the most appropriate expression system depending on the yield and the project requirements.
  • Mice injections (5 mice)
  • Immune response control in ELISA
  • Selection of the best two mice for two fusions, in order to have a large number and variety of clones
  • Screening of the fusion product by ELISA: around 10 x 96-well plates
  • Subcloning of 10 parental clones (2-3 rounds of limiting dilutions) examined by ELISA
  • Production and purification of 2mg from 10 clones
  • Conjugation of all 10 clones to Biotin, HRP or AP
  • Test of all the 90 sandwich possibilities
  • Level 1 : If we don’t succeed in producing an antibody pair that works in ELISA against the immunogen, we charge EUR 2500
  • Level 2 : If we succeed in producing an antibody pair that works in ELISA against the immunogen, we charge a small part of the cost only
  • Level 3 : If after testing the purified antibody the customer wants to receive the hybridoma, we charge the full package price
  • 10 -30 best clones at parental stage for customer’s selection
  • 100 µg of each purified antibody from best pair to confirm hybridoma purchase
  • 50 µg of purified protein
  After total payment:
  • Remaining purified and conjugated antibodies
  • 10 hybridoma cell line (four frozen vials)
  • 150 µg of purified protein
  • Gene in pUC57
  • Gene synthesis: 3-4 weeks
  • Protein production : 7-9 weeks
  • Injections: 8-12 weeks
  • Fusion & screening: 5-6 weeks
  • Subcloning: 12-18 weeks
  • Antibody production: 3-4 weeks
  • Conjugation and tests: 2 weeks
  • Total: 40-55 weeks