Chromosomal replication initiator protein DnaA

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Description of Chromosomal replication initiator protein DnaA

Description of Chromosomal replication initiator protein DnaA

General Information about Chromosomal replication initiator protein DnaA

This entry represents the central domain of bacterial DnaA protein, which plays an important role in initiating and regulating chromosome replication. DnaA is an ATP and DNA binding protein. It specifically binds to a 9 bp nucleotide repeat sequence, the dnaA box, which is found in the chromosomal origin of replication (oriC). DnaA is a protein of about 50kDa and contains two conserved regions: the first is located in the N-terminal half and corresponds to the ATP binding domain, and the second is located in the C-terminal half and may be involved in DNA binding. The protein can also bind RNA polymerase β subunit, dnaB and dnaZ proteins, and groE gene products (chaperone proteins).

Product nameChromosomal replication initiator protein DnaA
Expression systemProcaryotic expression
Molecular weight54.43kDa
Purity estimated>40% by SDS-PAGE
Buffer50 mM Tris-HCl pH 8.0, 150 mM NaCl/PBS pH 7.5, 4 M urea
Delivery conditionDry Ice
Storage condition4°C for short term (1 week), -20°C or -80°C for long term (avoid freezing/thawing cycles; addition of 20-40% glycerol improves cryoprotection)
BrandProteoGenix
Host speciesEscherichia coli (E.coli)
Fragment TypeMer1-472Ser
Aliases /Synonyms/
ReferencePX-P4506
NoteFor research use only
Product nameChromosomal replication initiator protein DnaA
Expression systemProcaryotic expression
Molecular weight54.43kDa
Purity estimated>40% by SDS-PAGE
Buffer50 mM Tris-HCl pH 8.0, 150 mM NaCl/PBS pH 7.5, 4 M urea
Delivery conditionDry Ice
Storage condition4°C for short term (1 week), -20°C or -80°C for long term (avoid freezing/thawing cycles; addition of 20-40% glycerol improves cryoprotection)
BrandProteoGenix
Host speciesEscherichia coli (E.coli)
Fragment TypeMer1-472Ser
Aliases /Synonyms/
ReferencePX-P4506
NoteFor research use only

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