MONOCOLONAL ANTIBODY PRODUCTION SERVICES
ProteoGenix developed XtenCHOTM, the most productive expression system for transient monoclonal antibody production, even exceeding ExpiCHO performances! Based on years of research and tailored to meet your requirements, our proprietary XtenCHOTM cell line gives you access to a large amount of your antibody in a short time period, all at the most competitive price of the market!
Why choose ProteoGenix for your monoclonal
XtenCHOTM > ExpiCHO
Our proprietary expression system even exceeds ExpiCHO’s production yields!
Most competitive price
590 € for 1mg purified antibody* thanks to XtenCHOTM outstanding performances!
We guarantee the amount ordered following well-established pricing guidelines!
Expression system diversity
Produce your antibodies in HEK or CHO expression systems.
Antibody engineering experts
Humanization, affinity maturation, bispecific antibody development… Generate antibody variants that can bring your project far further!
From your gene sequence to your antibody in 3-4 weeks!
Our recombinant monoclonal antibody production
|Monoclonal antibody sequencing||
||2 to 3 weeks for variable region & leader sequence
3 to 4 weeks for full length antibody
|Gene synthesis and cloning||
||2 to 3 weeks||
|Transient monoclonal antibody production and purification||
||Starting from 3 to 4 weeks||
Options available (non exhaustive list):
XtenCHOTM: Gold standard expression system for transient
monoclonal antibody production
ProteoGenix proposes to its customers a unique high performance transient expression system. Thanks to our in-house Xten transfection protocol and our proprietary XtenCHOTM cell line, our mammalian expression system even exceeds the performances of ExpiCHO, the most productive transient expression cell line on the market.
Our Xten expression system is our answer to all your transient recombinant antibody production challenges and significantly reduces the cost of production.
Here are some examples of the incredible results we obtained for the transient expression of Pembrolizumab and of a human IgG.
Comparison of a human IgG expression yield in three different expression systems
including XtenCHOTM using Xten transient protocol.
Comparison of the Pembrolizumab expression yield in three different expression
systems including XtenCHOTM using Xten transient protocol.
Pembrolizumab recombinant production
Aim of the study
Here, we showcase the recombinant Pembrolizumab production using our proprietary Xten expression system.
Genes and Expression Vectors
The genes coding for the Heavy chain (HC) and the light chain (LC) of the therapeutic antibody Pembrolizumab were chemically synthesized with optimizations for expression in CHO cells. The genes were cloned in ProteoGenix’s proprietary mammalian cells expression vector pTXs1. The vector map is provided in the PDF report.
Small-scale antibody production and purification tests
An endotoxin-free DNA preparation was done for the pTXs1 expression constructions. By using the proprietary Xten transfection protocol, the plasmids were then transiently transfected in the proprietary XtenCHOTM cells. Culture medium was collected and production yield was determined by Octet Red analysis. The recombinant antibody was then purified by affinity vs. protein G.
QC of the purified Pembrolizumab. Coomassie blue staining.
A. Non-reduced SDS-PAGE. B. Reduced SDS-PAGE.
Pembrolizumab antibody expression results
What are the benefits of recombinant
monoclonal antibody production?
Monoclonal antibody production by recombinant expression technology has been a major breakthrough in the therapeutic antibody development context as it leads to several advantages over the “traditional” antibody production method from hybridoma.
Reproducibility, control and security
Monoclonal antibody production for therapeutic applications requires high control and reproducibility. Because crucial parameters such as DNA and protein sequence are chemically defined, recombinant antibody production allows creating a standardized process for monoclonal antibody production with very low batch-to-batch variations. As recombinant antibody production starts from a known antibody or DNA sequence, mutations or cell-line drift that can occur in hybridoma cell lines are also obviated.
Recombinant antibody production leads to high purity grade due to the absence of serum contaminants.
Engineering and reformatting
Recombinant monoclonal antibody production allows getting access to the control of the antibody structure at the genetic level. This is particularly important when it comes to therapeutic antibody development as it permits to modulate the pharmacological properties of an antibody.
The possibility to modify the gene that will further be expressed by a selected expression system gives also access to an infinite range of solutions in terms of:
- Reformatting: species, isotype or subtype switching
- Antibody engineering: humanization, affinity maturation, bispecific antibody, antibody drug conjugates (ADC), antibody fragments (Fab, Fab2, scFv).
No animal use
Today, more and more attention is dedicated to ethics and animal welfare. Thanks to recombinant production technology, the antibody production process can be completely without animal use.
Transient or stable? Which monoclonal antibody
production service should you choose?
Choosing the most appropriate solution for your monoclonal antibody production can have a major impact on your project, for instance in terms of time. For this reason, we put at your disposal this short section explaining how to decide between transient or stable antibody expression.
Transient monoclonal antibody production offers a main advantage: it allows rapid production of a relatively large amount of antibody. However, in transient transfection, the transfected gene does not integrate the host genome and can be lost in several days (over a short time period, several days). Thus, it can be the perfect solution for short-term applications such as small-scale protein production.
Stable cell line development is related to the integration of a synthetic gene into the host genome of the expression system. As it can be a long and complicated development process (>3 months), stable cell lines are privileged for long-term applications such as therapeutic antibody production. For large scale bioproduction, stable cell line allows to construct a standardized process which guarantees higher yield and quality control.
For any additional question, please feel free to contact our PhD account manager who will be glad to guide you toward the most suitable antibody production solution.
*at least 4 antibodies