Recombinant Human TRPV6/ECaC2 Protein, N-His

Description of Recombinant Human TRPV6/ECaC2 Protein, N-His

Introduction

Recombinant Human TRPV6/ECaC2 Protein, also known as transient receptor potential vanilloid 6 and epithelial calcium channel 2, is a protein that plays a crucial role in calcium homeostasis and absorption in the body. This protein is encoded by the TRPV6 gene and is expressed predominantly in the epithelial cells of the small intestine, colon, and kidney. In this article, we will discuss the structure, activity, and applications of Recombinant Human TRPV6/ECaC2 Protein.

Structure of Recombinant Human TRPV6/ECaC2 Protein

Recombinant Human TRPV6/ECaC2 Protein is a transmembrane protein with a molecular weight of approximately 100 kDa. It consists of 801 amino acids and contains six transmembrane domains, with a large intracellular loop between the fifth and sixth transmembrane domains. The N- and C-termini of the protein are located in the cytoplasm. The structure of this protein is similar to other members of the transient receptor potential (TRP) family of ion channels.

Activity of Recombinant Human TRPV6/ECaC2 Protein

Recombinant Human TRPV6/ECaC2 Protein functions as a calcium-selective ion channel, allowing for the transport of calcium ions across the cell membrane. It is activated by both intracellular and extracellular factors, including hormones, growth factors, and vitamin D. The activation of this protein leads to an increase in intracellular calcium levels, which is essential for many cellular processes such as cell signaling, muscle contraction, and bone formation.

Regulation of Recombinant Human TRPV6/ECaC2 Protein

The activity of Recombinant Human TRPV6/ECaC2 Protein is tightly regulated to maintain calcium homeostasis in the body. This protein is regulated at both the transcriptional and post-translational levels. Hormones such as parathyroid hormone (PTH) and 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) can upregulate the expression of TRPV6, leading to an increase in calcium absorption. On the other hand, inflammatory cytokines can downregulate TRPV6 expression, reducing calcium absorption.

Applications of Recombinant Human TRPV6/ECaC2 Protein

Recombinant Human TRPV6/ECaC2 Protein has various applications in both research and clinical settings. Some of the key applications of this protein include:

1. Research on Calcium Homeostasis

Recombinant Human TRPV6/ECaC2 Protein is an essential tool for studying calcium homeostasis and absorption. Researchers can use this protein to investigate the role of TRPV6 in calcium transport and its regulation by various factors. It can also be used to study the effects of mutations in the TRPV6 gene on calcium absorption and related diseases.

2. Development of Therapeutics

The dysregulation of calcium homeostasis is associated with various diseases, including osteoporosis, kidney stones, and cancer. Recombinant Human TRPV6/ECaC2 Protein can be used to develop therapeutics that target this protein and modulate its activity. For example, inhibitors of TRPV6 may be useful in treating hypercalcemia, while activators of TRPV6 may help in treating calcium deficiency disorders.

3. Diagnostic Marker

Abnormal levels of TRPV6 expression have been observed in certain diseases, making it a potential diagnostic marker. For instance, increased TRPV6 expression has been linked to prostate cancer, while decreased expression has been associated with inflammatory bowel disease. Measuring TRPV6 levels in patients could aid in early diagnosis and monitoring of these diseases.

4. Vaccine Development

Recombinant Human TRPV6/ECaC