Recombinant Human QKI Protein, N-His

Description of Recombinant Human QKI Protein, N-His

Introduction to Recombinant Human QKI Protein

Recombinant Human QKI Protein, also known as Quaking Protein, is a highly conserved RNA-binding protein that plays a crucial role in regulating gene expression and post-transcriptional processing of RNA. It is a member of the STAR (Signal Transduction and Activation of RNA) family of proteins and is encoded by the QKI gene located on chromosome 6 in humans.

Structure of Recombinant Human QKI Protein

The Recombinant Human QKI Protein is a 315 amino acid protein with a molecular weight of approximately 34 kDa. It consists of three major domains: an N-terminal STAR domain, a central KH domain, and a C-terminal QUA2 domain. The STAR domain is responsible for RNA binding and contains two RNA recognition motifs (RRMs). The KH domain is involved in protein-protein interactions and the QUA2 domain is responsible for nuclear localization and homodimerization of the protein.

The crystal structure of Recombinant Human QKI Protein has been determined and it is found to form a homodimer in its active form. The dimerization of QKI is essential for its RNA binding and regulatory functions. The protein is also post-translationally modified by phosphorylation and acetylation, which can modulate its activity and stability.

Activity of Recombinant Human QKI Protein

Recombinant Human QKI Protein is a multifunctional protein that has been shown to play a critical role in a variety of cellular processes, including RNA splicing, stability, and localization. It is involved in the regulation of alternative splicing, which is a process that generates multiple mRNA isoforms from a single gene. QKI binds to specific RNA sequences and controls the splicing of target genes, thereby regulating their expression levels and functions.

In addition to its role in splicing, Recombinant Human QKI Protein also regulates the stability and localization of target mRNAs. It binds to the 3’ untranslated region (UTR) of target mRNAs and promotes their stability and transport to specific subcellular compartments. This is crucial for the proper functioning of cells, as misregulation of mRNA stability and localization can lead to various diseases, including cancer.

Moreover, Recombinant Human QKI Protein has been shown to interact with other proteins involved in RNA processing and translation, such as the RNA helicase DHX9 and the translation initiation factor eIF4E. These interactions further highlight the role of QKI in regulating gene expression at multiple levels.

Application of Recombinant Human QKI Protein

The unique RNA-binding and regulatory functions of Recombinant Human QKI Protein make it a valuable tool for various research applications. It has been extensively studied in the context of neurodevelopment and neurodegenerative diseases, as QKI is highly expressed in the brain and has been implicated in the regulation of neuronal differentiation and myelination.

Furthermore, Recombinant Human QKI Protein has been shown to be involved in the development and progression of various cancers, including breast, lung, and prostate cancer. Its role in regulating alternative splicing and mRNA stability of oncogenes and tumor suppressor genes makes it a potential target for cancer therapy.

In addition, Recombinant Human QKI Protein has been shown to play a crucial role in the pathogenesis of multiple sclerosis (MS), a chronic inflammatory disease of the central nervous system. QKI has been found to regulate the differentiation and function of oligodendrocytes, the cells responsible for myelination of neurons. Dysregulation of QKI has been linked to the development of MS, making it a potential therapeutic target for this disease.

Conclusion

In summary, Recombinant Human QKI Protein is a multifunctional protein with crucial roles in regulating gene expression and post-transcriptional processing of RNA. Its unique structure and