Datasheet of Polymorphprep™
Polymorphprep™ is a ready-made, sterile and endotoxin tested solution used to isolate polymorphonuclear granulocytes from blood. It is the ideal medium for neutrophils and eosinophils.
The mononuclear and the polymorphonuclear leucocytes are separated into two distinct bands free from red blood cells.
The temperature of the blood sample and the medium should be kept between 18-22°C.
Temperature variations may affect the density and the viscosity of Polymorphprep™ solution which may lead to poor outcome.
Please note: The method is effective only with whole undiluted blood not with a leukocyte-rich fraction.
|Delivery condition||Room temperature|
|Delivery lead time in business days||• Europe: 1-2 days |
• USA& Canada: 2-3 days
• Rest of the World: 3-7 days
Storage and Stability Conditions of Polymorphprep™
• Polymorphprep™ should be stored at room temperature (20°C).
• It’s stable for 3 years provided the solution is kept sterile and protected from light.
• Prolonged exposure to direct sunlight leads to release of iodine from the Sodium Diatrizoate molecule (this effect is negligible when working with the solution on a day to day basis.)
|Applications||Isolation of human polymorphonuclear cells|
|Composition||The solution contains Sodium Diatrizoate and Dextran 500 in the following concentrations: |
• Sodium diatrizoate 13.8% (w/v)
• Dextran 500 8.0 (w/v)
|Principle of the separation procedure|
How Polymorphprep™ works
Polymorphonuclear leukocytes (PMNs) has a lower buoyant density than erythrocytes (1.09-1.11 g/ml) but a higher buoyant density than the mononuclear cells (higher than 1.085 g/ml). Therefore, it is difficult to isolate polymorphonuclear granulocytes using a density barrier similar to that used for mononuclear cell. Several procedures have been developed to overcomes these issues.
The high osmolality of Polymorphprep™ causes erythrocytes to lose water and shrink, thus increasing their effective buoyant densities. This allows the dextran aggregated erythrocytes to sediment rapidly through the dense medium.
As the cells sediment further into the medium, the osmotic gradient between the medium and the erythrocytes declines. This means that water loss from the erythrocytes is greater at the top of the Polymorphprep™ and progressively decreases as they sediment further. A gradient of diatrizoate is formed within the density barrier. The PMNs band within this density gradient while the mononuclear cells remain at the sample/medium interface.
Polymorphprep™ vs Ficoll-Paque
• Ficoll-Paque (GE Health Care) is not endotoxin tested at a base level and higher price
• Unlike Polymorphprep, Ficoll-Paque is not manufactured under EU cGMP compliance.
|Alternatives||Polymorphprep™ can be a good substitute for Ficoll-Paque|
|Related Products||• OptiPrep: The ideal density gradient medium|
• Lymphoprep: Isolation of human mononuclear cells
• Lymphoprep Tube: Isolation of human mononuclear cells
• Nycodenz: A universal density gradient medium
Publication mentioning Polymorphprep™
1. Ferrante, A. & Thong, Y.H. (1980): Optimal conditions for simultaneous purification of mononuclear and polymorphonuclear leucocytes from human peripheral blood by Ficoll-Hypaque method. J. Immunol. Methodes 36,109
2. Sofia Tedelind and al. (2007) Anti-inflammatory properties of the short-chain fatty acids acetate and propionate: A study with relevance to inflammatory bowel disease
3. Iwaniuk A, Jab?o?ska E, Jab?o?ski J, Ratajczak-Wrona W, Garley M.2015. Expression of selected proteins of the extrinsic and intrinsic pathways of apoptosis in human leukocytes exposed to N-nitrosodimethylamine. Hum Exp Toxicol. 6:591–600