Lymphoprep™ should be stored at room temperature. It should be kept sterile and protected from light. Under these conditions Lymphoprep™ can be used for up to 3 years. Prolonged exposure to direct sunlight leads to release of iodine from the sodium diatrizoate molecule. This effect is negligible when working with this solution on a day to day basis.
Brand
Axis-Shield/Serumwerk
Composition
The solution contains sodium diatrizoate and polysaccharide in the following concentrations: Sodium Diatrizoate 9.1% (w/v) Polysaccharide 5.7% (w/v)
Principle of the separation procedure
Prior to Lymphoprep, the most common technique for separating leucocytes is to mix blood with a compound which aggregates the erythrocytes, thereby increasing their sedimentation rate. The sedimentation of leucocytes is only slightly affected and can be collected from the upper part of the tube when the erythrocytes have settled. Using a mixture of sodium metrizoate and polysaccharide – initial version of Lymphoprep™ - Bøyum (1968) developed a one-step centrifugal technique for isolation of lymphocytes. Thorsby and Bratlie (1970) used this technique with only slight modifications in the preparation of pure lymphocyte suspension for cytotoxicity tests and lymphocyte cultures. As emphasized also by other authors, Harris and Ukayiofo (1969), Ting and Morris (1971), Lymphoprep™ is a reliable, simple and quick method suitable for the preparation of lymphocyte preparations from cadaver blood, and from anticoagulated blood stored at room temperature for up to 6 hours.
Advantages
Lymphoprep™ vs Ficoll-Paque & Histopaque 1077
• Low endoxin • Less expensive • Lymphoprep™ is the only product of its kind produced under GMP conditions • Can be a good substitute for Ficoll-Paque™ with no further change of the existing protocols
Alternatives
Lymphoprep™ can be a good substitution to:
Ficoll® Ficoll-Paque Histopaque Buffy booster Cell separation media
Reference
1114544
Description of Lymphoprep™
Lymphoprep™ is a ready-to-use, sterile and endotoxin-tested density gradient medium designed for the isolation of mononuclear cells from peripheral blood, cord blood, and bone marrow based on differences in cell density.
During density gradient centrifugation, granulocytes and erythrocytes sediment through the Lymphoprep™ layer due to their higher density, while mononuclear cells form a distinct band at the plasma–medium interface. This enables efficient separation and recovery of viable lymphocytes and monocytes.
Lymphoprep™ is widely used for the preparation of high-purity lymphocyte suspensions for applications such as:
Tissue typing
Production of antilymphocyte sera
Immunological and cellular research
The Lymphoprep™ separation method is rapid, simple, and reliable, delivering consistent results with blood samples from both healthy donors and patients.
Why Buy Lymphoprep™ from ProteoGenix?
Choosing ProteoGenix ensures reliable access to high-quality laboratory reagents and responsive scientific support.
✔ Competitive pricing compared with major laboratory distributors
✔ Fast worldwide delivery to research laboratories and biotech companies
✔ Reliable laboratory-grade quality suitable for research applications
✔ Dedicated scientific support from experienced specialists
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