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Elizabethkingia miricola PNGaseF Recombinant Protein
| Size | 15000u, 75000u, 10ug, 100ug |
|---|---|
| Brand | |
| Product type | |
| Host Species |
Description of Elizabethkingia miricola PNGaseF Recombinant Protein
Description of Elizabethkingia miricola PNGaseF Recombinant Protein
Description of Elizabethkingia miricola PNGaseF Recombinant Protein
Description of Elizabethkingia miricola PNGaseF Recombinant Protein
General information on Elizabethkingia miricola PNGaseF Recombinant Protein-15000u
PNGase F is a recombinant glycosidase cloned from Elizabethkingia miricola and overexpressed in E. coli. It cleaves a complete glycan from a glycoprotein and it deaminates the asparagine to aspartic acid, but leaves the oligosaccharide undamaged. PNGase F will not eliminate oligosaccharides containing Alpha-(1, 3)-linked core fucose usually found in plant glycoproteins. A tri-peptide with the oligosaccharide-linked asparagine as the central residue is the minimal substrate for PNGase F.
| Product name | Elizabethkingia miricola PNGaseF Recombinant Protein |
|---|---|
| Origin species | Elizabethkingia miricola |
| Expression system | Procaryotic expression |
| Molecular weight | 34.78 kDa |
| Purity estimated | 95% |
| Buffer | Tris-HCl 20mM,NaCl 50mM, EDTA 5mMl, pH7.5 |
| Form | Frozen |
| Delivery condition | Dry Ice |
| Storage condition | 4°C for short term (1 week), -20°C or -80°C for long term (avoid freezing/thawing cycles; addition of 20-40% glycerol improves cryoprotection) |
| Brand | ProteoGenix |
| Host species | Escherichia coli (E.coli) |
| Fragment Type | Full-length |
| Aliases /Synonyms | Peptide -N-Glycosidase F, PNGase F, PNGaseF |
| Reference | PX-P3070 |
| Publications | 1: Kuhn P, Tarentino AL, Plummer TH Jr, Van Roey P. Crystal structure of peptide-N4-(N-acetyl-beta-D-glucosaminyl)asparagine amidase F at 2.2-A resolution. Biochemistry. 1994 Oct 4;33(39):11699-706. PubMed PMID: 7918386. 2: Norris GE, Stillman TJ, Anderson BF, Baker EN. The three-dimensional structure of PNGase F, a glycosylasparaginase from Flavobacterium meningosepticum. Structure. 1994 Nov 15;2(11):1049-59. PubMed PMID: 7881905. 3: Kuhn P, Guan C, Cui T, Tarentino AL, Plummer TH Jr, Van Roey P. Active site and oligosaccharide recognition residues of peptide-N4-(N-acetyl-beta-D-glucosaminyl)asparagine amidase F. J Biol Chem. 1995 Dec 8;270(49):29493-7. PubMed PMID: 7493989. 4: Lemp D, Haselbeck A, Klebl F. Molecular cloning and heterologous expression of N-glycosidase F from Flavobacterium meningosepticum. J Biol Chem. 1990 Sep 15;265(26):15606-10. PubMed PMID: 2203781. 5: Barsomian GD, Johnson TL, Borowski M, Denman J, Ollington JF, Hirani S, McNeilly DS, Rasmussen JR. Cloning and expression of peptide-N4-(N-acetyl-beta-D-glucosaminyl)asparagine amidase F in Escherichia coli. J Biol Chem. 1990 Apr 25;265(12):6967-72. PubMed PMID: 2182635. 6: Tarentino AL, Quinones G, Trumble A, Changchien LM, Duceman B, Maley F, Plummer TH Jr. Molecular cloning and amino acid sequence of peptide-N4-(N-acetyl-beta-D-glucosaminyl)asparagine amidase from flavobacterium meningosepticum. J Biol Chem. 1990 Apr 25;265(12):6961-6. Erratum in: J Biol Chem 1990 Jul 5;265(19):11405. PubMed PMID: 2182634. |
| Note | For research use only |
| Product name | Elizabethkingia miricola PNGaseF Recombinant Protein |
|---|---|
| Origin species | Elizabethkingia miricola |
| Expression system | Procaryotic expression |
| Molecular weight | 34.78 kDa |
| Purity estimated | 95% |
| Buffer | Tris-HCl 20mM,NaCl 50mM, EDTA 5mMl, pH7.5 |
| Form | Frozen |
| Delivery condition | Dry Ice |
| Storage condition | 4°C for short term (1 week), -20°C or -80°C for long term (avoid freezing/thawing cycles; addition of 20-40% glycerol improves cryoprotection) |
| Brand | ProteoGenix |
| Host species | Escherichia coli (E.coli) |
| Fragment Type | Full-length |
| Aliases /Synonyms | Peptide -N-Glycosidase F, PNGase F, PNGaseF |
| Reference | PX-P3070 |
| Publications | 1: Kuhn P, Tarentino AL, Plummer TH Jr, Van Roey P. Crystal structure of peptide-N4-(N-acetyl-beta-D-glucosaminyl)asparagine amidase F at 2.2-A resolution. Biochemistry. 1994 Oct 4;33(39):11699-706. PubMed PMID: 7918386. 2: Norris GE, Stillman TJ, Anderson BF, Baker EN. The three-dimensional structure of PNGase F, a glycosylasparaginase from Flavobacterium meningosepticum. Structure. 1994 Nov 15;2(11):1049-59. PubMed PMID: 7881905. 3: Kuhn P, Guan C, Cui T, Tarentino AL, Plummer TH Jr, Van Roey P. Active site and oligosaccharide recognition residues of peptide-N4-(N-acetyl-beta-D-glucosaminyl)asparagine amidase F. J Biol Chem. 1995 Dec 8;270(49):29493-7. PubMed PMID: 7493989. 4: Lemp D, Haselbeck A, Klebl F. Molecular cloning and heterologous expression of N-glycosidase F from Flavobacterium meningosepticum. J Biol Chem. 1990 Sep 15;265(26):15606-10. PubMed PMID: 2203781. 5: Barsomian GD, Johnson TL, Borowski M, Denman J, Ollington JF, Hirani S, McNeilly DS, Rasmussen JR. Cloning and expression of peptide-N4-(N-acetyl-beta-D-glucosaminyl)asparagine amidase F in Escherichia coli. J Biol Chem. 1990 Apr 25;265(12):6967-72. PubMed PMID: 2182635. 6: Tarentino AL, Quinones G, Trumble A, Changchien LM, Duceman B, Maley F, Plummer TH Jr. Molecular cloning and amino acid sequence of peptide-N4-(N-acetyl-beta-D-glucosaminyl)asparagine amidase from flavobacterium meningosepticum. J Biol Chem. 1990 Apr 25;265(12):6961-6. Erratum in: J Biol Chem 1990 Jul 5;265(19):11405. PubMed PMID: 2182634. |
| Note | For research use only |
| Product name | Elizabethkingia miricola PNGaseF Recombinant Protein |
|---|---|
| Origin species | Schizosaccharomyces pombe |
| Expression system | Procaryotic expression |
| Molecular weight | 45,52 kDa |
| Purity estimated | 90% |
| Buffer | PBS, imidazole 300mM |
| Form | liquid |
| Delivery condition | Dry Ice |
| Storage condition | 4°C for short term (1 week), -20°C or -80°C for long term (avoid freezing/thawing cycles; addition of 20-40% glycerol improves cryoprotection) |
| Brand | ProteoGenix |
| Host species | Escherichia coli (E.coli) |
| Fragment Type | Partial |
| Aliases /Synonyms | Peptide -N-Glycosidase F, PNGase F, PNGaseF |
| Reference | PX-P3070 |
| Publications | 1: Kuhn P, Tarentino AL, Plummer TH Jr, Van Roey P. Crystal structure of peptide-N4-(N-acetyl-beta-D-glucosaminyl)asparagine amidase F at 2.2-A resolution. Biochemistry. 1994 Oct 4;33(39):11699-706. PubMed PMID: 7918386. 2: Norris GE, Stillman TJ, Anderson BF, Baker EN. The three-dimensional structure of PNGase F, a glycosylasparaginase from Flavobacterium meningosepticum. Structure. 1994 Nov 15;2(11):1049-59. PubMed PMID: 7881905. 3: Kuhn P, Guan C, Cui T, Tarentino AL, Plummer TH Jr, Van Roey P. Active site and oligosaccharide recognition residues of peptide-N4-(N-acetyl-beta-D-glucosaminyl)asparagine amidase F. J Biol Chem. 1995 Dec 8;270(49):29493-7. PubMed PMID: 7493989. 4: Lemp D, Haselbeck A, Klebl F. Molecular cloning and heterologous expression of N-glycosidase F from Flavobacterium meningosepticum. J Biol Chem. 1990 Sep 15;265(26):15606-10. PubMed PMID: 2203781. 5: Barsomian GD, Johnson TL, Borowski M, Denman J, Ollington JF, Hirani S, McNeilly DS, Rasmussen JR. Cloning and expression of peptide-N4-(N-acetyl-beta-D-glucosaminyl)asparagine amidase F in Escherichia coli. J Biol Chem. 1990 Apr 25;265(12):6967-72. PubMed PMID: 2182635. 6: Tarentino AL, Quinones G, Trumble A, Changchien LM, Duceman B, Maley F, Plummer TH Jr. Molecular cloning and amino acid sequence of peptide-N4-(N-acetyl-beta-D-glucosaminyl)asparagine amidase from flavobacterium meningosepticum. J Biol Chem. 1990 Apr 25;265(12):6961-6. Erratum in: J Biol Chem 1990 Jul 5;265(19):11405. PubMed PMID: 2182634. |
| Note | For research use only |
| Product name | Elizabethkingia miricola PNGaseF Recombinant Protein |
|---|---|
| Origin species | Schizosaccharomyces pombe |
| Expression system | Procaryotic expression |
| Molecular weight | 45,52 kDa |
| Purity estimated | 90% |
| Buffer | PBS, imidazole 300mM |
| Form | liquid |
| Delivery condition | Dry Ice |
| Storage condition | 4°C for short term (1 week), -20°C or -80°C for long term (avoid freezing/thawing cycles; addition of 20-40% glycerol improves cryoprotection) |
| Brand | ProteoGenix |
| Host species | Escherichia coli (E.coli) |
| Fragment Type | Partial |
| Aliases /Synonyms | Peptide -N-Glycosidase F, PNGase F, PNGaseF |
| Reference | PX-P3070 |
| Publications | 1: Kuhn P, Tarentino AL, Plummer TH Jr, Van Roey P. Crystal structure of peptide-N4-(N-acetyl-beta-D-glucosaminyl)asparagine amidase F at 2.2-A resolution. Biochemistry. 1994 Oct 4;33(39):11699-706. PubMed PMID: 7918386. 2: Norris GE, Stillman TJ, Anderson BF, Baker EN. The three-dimensional structure of PNGase F, a glycosylasparaginase from Flavobacterium meningosepticum. Structure. 1994 Nov 15;2(11):1049-59. PubMed PMID: 7881905. 3: Kuhn P, Guan C, Cui T, Tarentino AL, Plummer TH Jr, Van Roey P. Active site and oligosaccharide recognition residues of peptide-N4-(N-acetyl-beta-D-glucosaminyl)asparagine amidase F. J Biol Chem. 1995 Dec 8;270(49):29493-7. PubMed PMID: 7493989. 4: Lemp D, Haselbeck A, Klebl F. Molecular cloning and heterologous expression of N-glycosidase F from Flavobacterium meningosepticum. J Biol Chem. 1990 Sep 15;265(26):15606-10. PubMed PMID: 2203781. 5: Barsomian GD, Johnson TL, Borowski M, Denman J, Ollington JF, Hirani S, McNeilly DS, Rasmussen JR. Cloning and expression of peptide-N4-(N-acetyl-beta-D-glucosaminyl)asparagine amidase F in Escherichia coli. J Biol Chem. 1990 Apr 25;265(12):6967-72. PubMed PMID: 2182635. 6: Tarentino AL, Quinones G, Trumble A, Changchien LM, Duceman B, Maley F, Plummer TH Jr. Molecular cloning and amino acid sequence of peptide-N4-(N-acetyl-beta-D-glucosaminyl)asparagine amidase from flavobacterium meningosepticum. J Biol Chem. 1990 Apr 25;265(12):6961-6. Erratum in: J Biol Chem 1990 Jul 5;265(19):11405. PubMed PMID: 2182634. |
| Note | For research use only |
Category:Recombinant Proteins
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