Size exclusion chromatography is based on separating different-sized molecules according to their molecular weights or sizes. Unlike other chromatography methods, the former technique uses mild conditions and is usually employed for improved purity at the end of the downstream process. As all our protein purification techniques, Size Exclusion Chromatography (SEC) can be included in our protein expression services.
At the earliest stages of your project, ProteoGenix establishes your full protein expression strategy, from gene design to protein purification. In this context, SEC purification can be recommended when:
Size exclusion chromatography, also known as Gel-Filtration chromatography is a type of liquid chromatography. It allows the separation of molecules based on their size or molecular weight.
Gel-filtration chromatography is equipped with a resin composed of porous particles/beads. Different in size, these pores grant the size separation property of this technique. These beads are chemically and physically stable. Besides, they do not interact with the surrounding solution. However, thanks to their pores they enable the entry or not of molecules based on their size.
Gel-filtration is composed of a mobile phase as well as a stationary phase:
Size exclusion chromatography is a versatile method. The flexibility of the conditions allows to use it for several applications, for instance:
In conclusion, size exclusion chromatography can result in yield loss due to sample dilution, but it remains an efficient method for the separation of molecules based on their size. For this reason, this method is recommended to be used at the end of the purification process. SEC purification is also well-known to increase the product’s purity in mild conditions and operate molecule separation under flexible conditions.
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