Have you ever wasted time because of bad immunoprecipitation antibodies? Don’t let this happen again! At ProteoGenix, we develop your antibody for immunoprecipitation and we guarantee that it will work in your own conditions.

How could you be sure that your antibodies will work in your immunoprecipitation experiment?

Making the right decisions starts with the choice of the best tools. At ProteoGenix, we understand that your experiments are time-consuming and that their success is essential to move your projects forward. If you don’t want to waste your time, our immunoprecipitation guaranteed antibodies are the best solution because:

  1. We manage the whole antibody development process from antigen design to hybridoma and antibody production
  2. We test your antibodies in your conditions
  3. We send you part of the sample so that you can validate it after testing it in your own lab

Ready to start? Contact our PhD account managers who will guide you during your project!

Step Content Timeline Deliverables
Antigen production
  • Antigen design
  • Gene synthesis
  • Test of two expression systems
  • Antigen production
10 to 13 weeks
Mice immunization
  • Mice injections
  • Immune response control by FC
8 to 12 weeks
Hybridoma production
  • Mice selection
  • Fusion of B cells with myeloma cells
  • Screening of the fusion products by ELISA
  • Screening of the positive clones by FC
  • pecificity control
5 to 6 weeks
  • 10-20 best clones for customer’s selection
Antibody production
  • Subcloning of two parental clones
  • Antibody production and purification
  • QC by FC
11 to 16 weeks
  • 100µg of purified antibody + 50µg of purified protein to confirm hybridoma purchase

Immunoprecipitation principle

Immunoprecipitation is an antibody-antigen interaction based technique used to isolate or concentrate a target protein in a complex sample (cell lysate…).

Immunoprecipitation relies on the affinity between an antibody and its target antigen. The general principle consists in incubating highly specific antibodies in a complex sample so that it can bind the target protein. The antibody-protein complex formed can be isolated thanks to antibody binding proteins (Protein A or Protein G or a secondary antibody detecting the primary antibody) attached to magnetic beads or agarose beads.

Two methodologies are commonly used:

  • Pre-immobilization of the antibodies on the solid substrate: in this methodology, antibodies are immobilized on a solid support (agarose or magnetic beads) prior incubation in the complex sample. The incubation period allows the binding of the target antigen to the immobilized antibody. These complexes can further be isolated and eluted from the solid support to be analyzed.
  • Pre-addition of the antibodies in the sample: this technique refers to the addition of free antibodies (unbound) in the complex sample. This allows forming an antibody-antigen complex before the addition of a solid substrate. This approach is generally preferred for low concentration target antigen or when the antibody-antigen interaction is weak or presents slow kinetics.