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Immobilized EGFR protein - Epidermal Growth Factor proteins receptor(EGFR) (cat. No.PX-P4586) at 0.5µg/mL (100µL/well) can bind to Cetuximab Biosimilar - Anti-hEGFR mAb (cat. No.PX-TA1019) in indirect ELISA with Goat Anti-Human IgG secondary antibody coupled with HRP measured by OD450
Cetuximab Biosimilar - Anti-hEGFR mAb - Research Grade on SDS-PAGE under reducing and non-reducing conditions. The gel was stained overnight with Coomassie Blue.. The purity of the antibody is greater than 95%.
Flow-cytometry using APC anti-human EGFR antibody. A431 cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human EGFR monoclonal antibody (Catalog: PX-TA1019, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Target Cells were stained with an irrelevant antibody or Cetuximab Biosimilar - Anti-hEGFR mAb - Research Grade at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Goat Anti-Human IgG Polyclonal Antibody, FITC (PTX18862) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry using PE anti-human EGFR antibody. A431 cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human EGFR monoclonal antibody (Catalog: PX-TA1019, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry using FITC anti-human EGFR antibody. A431 cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human EGFR monoclonal antibody (Catalog: PX-TA1019, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
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PTX18889
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