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Recombinant Human CD3E Protein, N-His(cat. No.ARO-P10225) at 0.5µg/mL (100µL/well) can bind Human CD3E (OKT3) Monoclonal Antibody in indirect ELISA with Goat secondary antibody measured by OD450.
Target Cells were stained with an irrelevant antibody or Human CD3E (OKT3) Monoclonal Antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Goat Anti-Human IgG Polyclonal Antibody, FITC (PTX18862) and cells analysed on a NovoCyte Flow Cytometer.
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PTX17816
PTX17818
ARO-P10225
PTX20654
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