4°C for short term (1 week), -20°C or -80°C for long term (avoid freezing/thawing cycles; addition of 20-40% glycerol improves cryoprotection)
Brand
ProteoGenix
Host species
Insect
Fragment Type
Full-length
Protein Accession
NP_476656.1
Spec:Entrez GeneID
33271
Spec:NCBI Gene Aliases
CG3966, NINAA, DmelCG3966, ninA, NinaA
Spec:SwissProtID
P15425
NCBI Reference
NP_476656.1
Aliases /Synonyms
VSVG, neither inactivation nor afterpotential A, Peptidyl-prolyl cis-trans isomerase, rhodopsin-specific isozyme, PPIase, Rotamase
Reference
PX-P2099
Note
For research use only
Description of VSVG Protein- Drosophila VSVG NJ Recombinant Protein
General Information on VSVG NJ Protein:
Vesicular stomatitis virus (VSV) glycoproteins mediate both cell attachment and membrane fusion. Unlike many other low-pH-induced viral fusion proteins, their fusogenic conformational transitions is reversible. VSV-G has a three-stage fusion kinetics:
I. G-protein conformational change which is reversible and pH-dependent.
II. Reversible trimerization and clustering of the G-protein fusion loops.
III. Folding back of a cluster of extended trimers into their post-fusion conformations.
VSV NJ glycoprotein contains 517 amino acids and is glycosylated at position 178 and 335. Unlike VSV Indiana (VSIV), another major serotype of VSV, VSV NJ is not acylated. The two serotypes also differentiate in terms of antigenic structure. VSV NJ has a faster glycoprotein folding intracellularly and with less dependence on glycosylation.
1: Lenhard T, Reiländer H. Engineering the folding pathway of insect cells:
generation of a stably transformed insect cell line showing improved folding of a
recombinant membrane protein. Biochem Biophys Res Commun. 1997 Sep
29;238(3):823-30. PubMed PMID: 9325175.
2: Hemenway C, Heitman J. Proline isomerases in microorganisms and small
eukaryotes. Ann N Y Acad Sci. 1993 Nov 30;696:38-46. Review. PubMed PMID:
8109845.
3: Larrivee DC, Conrad SK, Stephenson RS, Pak WL. Mutation that selectively
affects rhodopsin concentration in the peripheral photoreceptors of Drosophila
melanogaster. J Gen Physiol. 1981 Nov;78(5):521-45. PubMed PMID: 6796648; PubMed
Central PMCID: PMC2228638.
4: Pak WL, Conrad SK, Kremer NE, Larrivee DC, Schinz RH, Wong F. Photoreceptor
function. Basic Life Sci. 1980;16:331-46. PubMed PMID: 6779798.
5: Schneuwly S, Shortridge RD, Larrivee DC, Ono T, Ozaki M, Pak WL. Drosophila
ninaA gene encodes an eye-specific cyclophilin (cyclosporine A binding protein).
Proc Natl Acad Sci U S A. 1989 Jul;86(14):5390-4. PubMed PMID: 2664782; PubMed
Central PMCID: PMC297628.
6: Shieh BH, Stamnes MA, Seavello S, Harris GL, Zuker CS. The ninaA gene required
for visual transduction in Drosophila encodes a homologue of cyclosporin
A-binding protein. Nature. 1989 Mar 2;338(6210):67-70. PubMed PMID: 2493138.
7: Webel R, Menon I, O'Tousa JE, Colley NJ. Role of asparagine-linked
oligosaccharides in rhodopsin maturation and association with its molecular
chaperone, NinaA. J Biol Chem. 2000 Aug 11;275(32):24752-9. PubMed PMID:
10811808.
9: Bentrop J, Schwab K, Pak WL, Paulsen R. Site-directed mutagenesis of highly
conserved amino acids in the first cytoplasmic loop of Drosophila Rh1 opsin
blocks rhodopsin synthesis in the nascent state. EMBO J. 1997 Apr 1;16(7):1600-9.
PubMed PMID: 9130705; PubMed Central PMCID: PMC1169764.
10: Ondek B, Hardy RW, Baker EK, Stamnes MA, Shieh BH, Zuker CS. Genetic
dissection of cyclophilin function. Saturation mutagenesis of the Drosophila
cyclophilin homolog ninaA. J Biol Chem. 1992 Aug 15;267(23):16460-6. PubMed PMID:
1644830.
11: Zuker CS, Mismer D, Hardy R, Rubin GM. Ectopic expression of a minor
Drosophila opsin in the major photoreceptor cell class: distinguishing the role
of primary receptor and cellular context. Cell. 1988 May 6;53(3):475-82. PubMed
PMID: 2966681.
12: Fouillet A, Levet C, Virgone A, Robin M, Dourlen P, Rieusset J, Belaidi E,
Ovize M, Touret M, Nataf S, Mollereau B. ER stress inhibits neuronal death by
promoting autophagy. Autophagy. 2012 Jun;8(6):915-26. doi: 10.4161/auto.19716.
Epub 2012 Jun 1. PubMed PMID: 22660271; PubMed Central PMCID: PMC3427257.
13: Mitra A, Chinchore Y, Kinser R, Dolph PJ. Characterization of two dominant
alleles of the major rhodopsin-encoding gene ninaE in Drosophila. Mol Vis.
2011;17:3224-33. Epub 2011 Dec 14. PubMed PMID: 22194648; PubMed Central PMCID:
PMC3244490.
14: Ahmad ST, Natochin M, Barren B, Artemyev NO, O'Tousa JE. Heterologous
expression of bovine rhodopsin in Drosophila photoreceptor cells. Invest
Ophthalmol Vis Sci. 2006 Sep;47(9):3722-8. PubMed PMID: 16936079.
15: Ozaki K, Nagatani H, Ozaki M, Tokunaga F. Maturation of major Drosophila
rhodopsin, ninaE, requires chromophore 3-hydroxyretinal. Neuron. 1993
Jun;10(6):1113-9. PubMed PMID: 8318232.
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