4°C for short term (1 week), -20°C or -80°C for long term (avoid freezing/thawing cycles; addition of 20-40% glycerol improves cryoprotection)
Brand
ProteoGenix
Host species
Escherichia coli (E.coli)
Fragment Type
Property sequence
Protein Accession
NP_734212.1
NCBI Reference
NP_734212.1
Aliases /Synonyms
GST-TEV, NIa-Pro protein (TEV protease)
Reference
PX-P1108
Note
For research use only
Description of Tobacco TEV Protease Recombinant Enzyme
General information on Tobacco TEV Protease Recombinant Enzyme:
TEV (Tobacco Etch Virus) Protease is a highly site-specific cysteine protease that is found in the Tobacco Etch Virus. Due to its high sequence specificity it is regularly used to cleave affinity tags from fusion proteins. The TEV encodes its whole genome as a single massive polyprotein. This is cleaved into functional units by the three proteases: P1 protease (1 cleavage site), helper-component protease (1 cleavage site) and TEV protease (7 cleavage sites). The greatest recognition site for this enzyme is the sequence Glu-Asn-Leu-Tyr-Phe-Gln-(Gly/Ser) [ENLYFQ/S] and cleavage appear between the Gln and Gly/Ser residues.
1: Martínez F, Daròs JA. Tobacco etch virus protein P1 traffics to the nucleolus _x000D_
and associates with the host 60S ribosomal subunits during infection. J Virol._x000D_
2014 Sep;88(18):10725-37. doi: 10.1128/JVI.00928-14. Epub 2014 Jul 2. PubMed_x000D_
PMID: 24991017; PubMed Central PMCID: PMC4178839.
_x000D_
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2: Cui X, Wei T, Chowda-Reddy RV, Sun G, Wang A. The Tobacco etch virus P3_x000D_
protein forms mobile inclusions via the early secretory pathway and traffics_x000D_
along actin microfilaments. Virology. 2010 Feb 5;397(1):56-63. doi:_x000D_
10.1016/j.virol.2009.11.015. Epub 2009 Nov 30. PubMed PMID: 19945728.
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3: Torres-Barceló C, Martín S, Daròs JA, Elena SF. From hypo- to_x000D_
hypersuppression: effect of amino acid substitutions on the RNA-silencing_x000D_
suppressor activity of the Tobacco etch potyvirus HC-Pro. Genetics. 2008_x000D_
Oct;180(2):1039-49. doi: 10.1534/genetics.108.091363. Epub 2008 Sep 9. PubMed_x000D_
PMID: 18780745; PubMed Central PMCID: PMC2567354.
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4: Allison RF, Dougherty WG, Parks TD, Willis L, Johnston RE, Kelly M, Armstrong _x000D_
FB. Biochemical analysis of the capsid protein gene and capsid protein of tobacco_x000D_
etch virus: N-terminal amino acids are located on the virion's surface. Virology._x000D_
1985 Dec;147(2):309-16. PubMed PMID: 18640560.
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