Brand: ProteoGenix

TNFa / TNF-alpha, N-His, recombinant protein

PX-P5961
Validated ELISA

Host species:: Mammalian cells
Origin species:: Homo sapiens (Human)
Uniprot ID:: P01375
Molecular weight:: 25.65 kDa

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His Val77–Leu233

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SDS-PAGE for TNFa / TNF-alpha, N-His, recombinant protein

TNFa / TNF-alpha, N-His, recombinant protein

Product name	TNFa / TNF-alpha, N-His, recombinant protein
Uniprot ID	P01375
Origin species	Homo sapiens (Human)
Expression system	Eukaryotic expression
Raw Antigen Sequence	VRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL
Molecular weight	25.65 kDa
Protein delivered with Tag?	N-terminal His Tag
Purity estimated	>90% as determined by SDS-PAGE
Buffer	PBS, pH7.5
Delivery condition	Dry Ice
Storage condition	4°C for short term (1 week), -20°C or -80°C for long term (avoid freezing/thawing cycles; addition of 20-40% glycerol improves cryoprotection)
Brand	ProteoGenix
Host species	Mammalian cells
Fragment Type	Val77-Leu233
Protein Accession	P01375
Aliases /Synonyms	cachexin, cachectin
Reference	PX-P5961
Note	For research use only
Molecular Constructor	His Val77–Leu233

General information on TNFa / TNF-alpha, N-His, recombinant protein

TNF-Alpha: Cytokine Protein

TNF-alpha, also known as tumor necrosis factor-alpha, is a cytokine protein produced mainly by macrophages and other white blood cells. It is involved in the regulation of immune responses, inflammation, and cell death. TNF-alpha binds to two receptors, TNFR1 and TNFR2, which are present on the surface of many cell types. Binding to TNFR1 activates the pro-inflammatory signals, while binding to TNFR2 leads to cell death.

TNF-Alpha: Antibody-Drug Target

TNF-alpha is a target for antibody-drugs used to treat autoimmune diseases such as rheumatoid arthritis, psoriasis, and Crohn’s disease. These drugs are monoclonal antibodies that bind to TNF-alpha and inhibit its activity, thus reducing inflammation and preventing tissue damage. Infliximab and adalimumab are two of the most commonly used TNF-alpha inhibitors.

TNF-Alpha: Application

TNF-alpha has also been studied for its potential therapeutic applications. It has been used in cancer therapy to induce apoptosis in tumor cells, and it has been studied as a potential treatment for HIV infection.

SDS-PAGE for TNFa / TNF-alpha, N-His, recombinant protein

TNFa / TNF-alpha, N-His, recombinant protein on SDS-PAGE under reducing condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is superior than 90 %.

Etanercept Biosimilar - Anti-TNF mAb binds to TNFa / TNF-alpha, N-His, recombinant protein in indirect ELISA Assay

Immobilized TNFa / TNF-alpha, N-His, recombinant protein (cat. No.PX-P5961) at 0.5µg/mL (100µL/well) can bind to Etanercept Biosimilar - Anti-TNF mAb (cat. No.PX-TA1015) in indirect ELISA with Goat Anti-Human IgG secondary antibody coupled with HRP measured by OD450