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Human Beta-nerve growth factor recombinant protein(cat. No.PX-P4027) at 0.5µg/mL (100µL/well) can bind InVivoMAb Anti-Human NGF/Beta-NGF (Iv0066) in indirect ELISA with Goat secondary antibody measured by OD450.
Flow-cytometry using anti-human NGF antibody.NCI-H460 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human NGF antibody monoclonal antibody (Catalog # ARO-A13308 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # PTX18862) and cells analysed on a NovoCyte Flow Cytometer.
Human Beta-nerve growth factor recombinant protein(cat. No.PX-P4027) can bind InVivoMAb Anti-Human NGF/Beta-NGF (Iv0066) in Western Blot Assay as detected on gel analysis.
InVivoMAb Anti-Human NGF/Beta-NGF (Iv0066), on SDS-PAGE. The gel was stained overnight with Coomassie Blue. The purity of the antibody is greater than 95%.
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