A guide on how to easily transition to a new antibody expression system kit

Why consider switching expression systems?

Switching or adding a new expression system kit should never be taken lightly, as it can have implications for costs and overall performance, from yield to the quality of the final product. However, several situations warrant considering this option.

Underperformance of your current expression system

Low yield

One common reason to consider a new expression system kit is dissatisfaction with the yields achieved using your current system. Low production yields directly increase production costs and negatively impact productivity. In my experience working in research labs, one of the most frustrating hurdles was slow progress due to inefficient protocols or the time required to generate starting materials.

Poor quality

Even if your current expression system kit achieves acceptable yields, it may fall short in quality. Specific post-translational modifications or secondary structures may not be optimal, preventing you from obtaining a biomolecule in its native, functional conformation. For example, if precise glycosylation is critical, a CHO-based system might be more appropriate.

Changing project requirements

Not all expression systems are suitable for every project. Some projects demand higher yields, others require co-expression of multiple proteins, or the expression of toxic proteins. Your project’s requirements and the biomolecule’s characteristics should guide your choice to retain or switch to a new expression system kit.

Scaling up production

As your project progresses, production needs may evolve. For instance, therapeutic antibody discovery often begins with small-scale testing of numerous candidates before scaling up production for a select few. In such cases, researchers often start with HEK systems for rapid transfection and small-scale production before transitioning to CHO systems for larger-scale production.

Pros and cons of switching expression systems

Advantages

Lower production costs

Switching to a more efficient expression system kit can significantly reduce production costs. For example, our XtenCHO Race™ system has been benchmarked against market-leading kits for therapeutic antibody production, offering cost advantages through superior consumable efficiency.

Improved biomolecule quality

Certain applications demand biomolecules in their native, functional forms. For instance, therapeutic antibody development requires antigens in their native conformation to ensure functional antibodies. A CHO-based expression system kit that mimics human post-translational modifications is essential in such cases.

Versatility across applications

Modern kits like XtenCHO Race™ and ExpiCHO™ offer excellent performance in yield, quality, and versatility. These kits are tested for various applications, from small-scale production (e.g., 96-well plates) to medium- and large-scale production (several grams of proteins or antibodies).

Disadvantages

Internal protocol adjustments

One of the most significant hurdles in adopting a new expression system kit is adapting internal protocols. Many labs rely on well-established workflows that are comfortable but might not be optimal. While the change may initially seem daunting, it can ultimately lead to better results.

Need for staff training

Switching kits often requires adapting to new culture conditions, transfection methods, or harvesting protocols. These changes involve a learning curve before achieving optimal results.

Additional equipment costs

Some kits, like those using agitated incubators, may require purchasing additional equipment. While this can be a considerable investment, shared facilities often provide cost-effective alternatives.

Tips for a smooth transition to a new expression system kit

Assess your needs and constraints

Identify your specific project requirements:

• Do you need high-yield production?
• Is native conformation or post-translational modification crucial?
• Is your biomolecule toxic?
• What is the end application of your project?

Choose a kit with clear, detailed protocols

Opt for kits with well-documented protocols to minimize the risk of failure. At ProteoGenix, we developed XtenCHO Race™ with this in mind, offering step-by-step guidance, tutorial videos for each day of use, and dedicated technical support with guaranteed response times of under 48 hours.

Check compatibility with your biomolecule

Ensure the new expression system kit has been used successfully with biomolecules similar to yours. XtenCHO Race™ is widely used for therapeutic antibody production and other mammalian-expressed proteins. Browse our product catalog or contact our team to learn more about its capabilities.

Test and compare systems

Test the new kit alongside your current system to compare results. This approach ensures you make an informed decision based on actual performance in your lab environment.

Decide based on data

Use a comparative table like the one below to evaluate whether switching systems is the right move:

Conclusion

Switching or adding a new expression system kit is a strategic decision that can significantly impact the success of your projects. Whether it’s to improve yields, optimize biomolecule quality, or adapt to new project requirements, making an informed choice is crucial.
At ProteoGenix, we’ve designed the XtenCHO Race™ kit to simplify this transition while maximizing your chances of success. With detailed protocols, dedicated technical support, and proven performance, we’re here to support you every step of the way.
So, why not take the first step toward increased productivity and efficiency?