Recombinant Human ELAVL3 Protein, N-His

Introduction to Recombinant Human ELAVL3 Protein

Recombinant Human ELAVL3 Protein, also known as ELAV-like protein 3, is a type of recombinant protein that plays a crucial role in various cellular processes. It is a member of the ELAV (embryonic lethal abnormal vision) family of RNA-binding proteins, which are known for their ability to regulate gene expression at the post-transcriptional level. In this article, we will explore the structure, activity, and applications of Recombinant Human ELAVL3 Protein.

Structure of Recombinant Human ELAVL3 Protein

The Recombinant Human ELAVL3 Protein is a 32 kDa protein consisting of 295 amino acids. It contains three RNA recognition motifs (RRMs) that are responsible for binding to specific RNA sequences. These RRMs are highly conserved in the ELAV family and are essential for their RNA-binding activity. The protein also has a nuclear localization signal, which allows it to be transported into the nucleus where it carries out its function.

The crystal structure of Recombinant Human ELAVL3 Protein has been determined and it reveals a compact protein with a globular shape. The three RRMs are arranged in a linear fashion, forming a groove that can accommodate the target RNA molecule. The structure also shows that the protein has a flexible C-terminal region, which is important for its RNA-binding activity.

Activity of Recombinant Human ELAVL3 Protein

Recombinant Human ELAVL3 Protein is primarily known for its role in regulating mRNA stability and translation. It binds to specific sequences in the 3′ untranslated region (UTR) of target mRNAs and protects them from degradation by ribonucleases. This allows the target mRNA to be translated into protein, leading to an increase in protein expression.

In addition to its role in mRNA stability, Recombinant Human ELAVL3 Protein also plays a role in alternative splicing. It has been shown to bind to pre-mRNA and influence the splicing process, resulting in the production of different protein isoforms. This activity is important for the regulation of gene expression and can have significant effects on cellular function.

Applications of Recombinant Human ELAVL3 Protein

The unique structure and activity of Recombinant Human ELAVL3 Protein make it a valuable tool in various research applications. Its ability to regulate mRNA stability and translation makes it an important factor in gene expression studies. Researchers can use this protein to manipulate the expression of specific genes and study their effects on cellular processes.

Recombinant Human ELAVL3 Protein is also used in alternative splicing studies. By manipulating its activity, researchers can investigate the effects of alternative splicing on protein function and cellular pathways. This can provide valuable insights into the role of alternative splicing in disease and other biological processes.

Furthermore, Recombinant Human ELAVL3 Protein has potential therapeutic applications. It has been shown to play a role in cancer progression, making it a potential target for cancer treatment. By understanding its structure and activity, researchers can develop drugs that can modulate its function and potentially inhibit cancer growth.

Conclusion

In summary, Recombinant Human ELAVL3 Protein is a versatile protein with a unique structure and activity. Its role in regulating mRNA stability and alternative splicing makes it a valuable tool in gene expression studies and has potential therapeutic applications. With ongoing research, we can continue to uncover the full potential of this protein and its impact on various cellular processes.