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CD62L recombinant protein(cat. No.PX-P5195) at 0.5µg/mL (100µL/well) can bind Human CD62L/SELL Monoclonal Antibody in indirect ELISA with Goat secondary antibody measured by OD450.
Flow-cytometry using anti-human SELL antibody. Human Jurkat cell line were stained with an irrelevant antibody (Green Histogram) or an anti-human SELL antibody monoclonal antibody (Catalog # ARO-A15777 , Red Histogram) at a concentration of 20 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # PTX18862) and cells analysed on a NovoCyte Flow Cytometer.
PTX17887
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