Human CD278/ICOS HEK293T Stable Cell Line

Introduction to Human CD278/ICOS HEK293T Stable Cell Line

Human CD278/ICOS (Inducible T-cell CO-Stimulator) is a protein that belongs to the CD28 family and is primarily expressed on activated T cells. It plays a crucial role in regulating immune responses by promoting the activation, differentiation, and survival of T cells. Dysregulation of CD278/ICOS signaling has been linked to various autoimmune diseases, making it an attractive therapeutic target. In order to study the structure, activity, and application of CD278/ICOS, a stable cell line has been developed using HEK293T cells.

Structure of Human CD278/ICOS

CD278/ICOS is a type I transmembrane protein with a molecular weight of approximately 50 kDa. It consists of an extracellular domain, a transmembrane domain, and a cytoplasmic tail. The extracellular domain contains an immunoglobulin (Ig)-like domain, which is essential for binding to its ligand, B7-H2. The cytoplasmic tail contains a conserved YMFM motif, which is responsible for recruiting signaling molecules and mediating downstream signaling pathways.

Activity of Human CD278/ICOS

CD278/ICOS is primarily expressed on activated T cells, but it can also be found on other immune cells such as regulatory T cells, B cells, and dendritic cells. Upon binding to its ligand, B7-H2, CD278/ICOS activates the PI3K/Akt and NF-κB signaling pathways, leading to the production of cytokines and the upregulation of co-stimulatory molecules. This results in the enhancement of T cell proliferation, survival, and effector functions.

Application of Human CD278/ICOS HEK293T Stable Cell Line

The Human CD278/ICOS HEK293T stable cell line has been developed to study the role of CD278/ICOS in immune responses and its potential as a therapeutic target. This stable cell line overexpresses CD278/ICOS, allowing for the study of its structure and function in a controlled environment. It can be used for various applications, including flow cytometry, functional assays, and drug screening.

Flow Cytometry

Flow cytometry is a powerful technique that allows for the analysis of cell surface markers. The Human CD278/ICOS HEK293T stable cell line can be used as a positive control in flow cytometry experiments to detect the expression of CD278/ICOS on activated T cells. It can also be used to study the binding of CD278/ICOS to its ligand, B7-H2, by co-staining with an antibody against B7-H2.

Functional Assays

The stable overexpression of CD278/ICOS in HEK293T cells allows for the study of its signaling pathways and downstream effects. Functional assays such as proliferation, cytokine production, and cell survival can be performed using this cell line. These assays can provide valuable insights into the role of CD278/ICOS in immune responses and its potential as a therapeutic target.

Drug Screening

Due to its involvement in various autoimmune diseases, CD278/ICOS has emerged as a promising therapeutic target. The Human CD278/ICOS HEK293T stable cell line can be used for drug screening to identify potential inhibitors or agonists of CD278/ICOS signaling. This can aid in the development of new therapies for autoimmune diseases.

Conclusion

In summary, the Human CD278/ICOS HEK293T stable cell line is a valuable tool for studying the structure, activity, and application of CD278/ICOS. Its stable overexpression of CD278/ICOS makes it a reliable model for various applications, including flow cytometry, functional assays, and drug screening.