Recombinant Human VDAC2 Protein, N-His

Introduction to Recombinant Human VDAC2 Protein

Recombinant Human VDAC2 Protein is a type of recombinant protein that is commonly used in scientific research and biotechnology. This protein is a member of the voltage-dependent anion channel (VDAC) family, which are transmembrane proteins that are involved in regulating ion and metabolite transport across the mitochondrial membrane. VDAC2 is a highly conserved protein found in all eukaryotic organisms, and its structure and function have been extensively studied due to its importance in cellular metabolism and apoptosis.

Structure of Recombinant Human VDAC2 Protein

Recombinant Human VDAC2 Protein is a 32-kDa protein that consists of 282 amino acids. It is composed of three beta-barrel transmembrane domains, with the N- and C-termini located on the cytosolic side of the mitochondrial membrane. The protein also contains six alpha-helices that are involved in forming the channel pore. VDAC2 is highly homologous to other VDAC family members, with VDAC1 and VDAC3 sharing 70% and 74% sequence identity, respectively.

Activity of Recombinant Human VDAC2 Protein

Recombinant Human VDAC2 Protein is primarily known for its role in regulating metabolite and ion transport across the mitochondrial membrane. It forms a channel that allows the passage of small molecules, such as ATP, ADP, and pyruvate, between the cytosol and the mitochondrial matrix. This channel is also permeable to ions, such as calcium and potassium, which play important roles in cellular metabolism and signaling.

In addition to its transport activity, VDAC2 has been shown to be involved in several other cellular processes. It has been implicated in the regulation of mitochondrial membrane potential, which is important for ATP production and cell survival. VDAC2 has also been linked to the release of cytochrome c from the mitochondria, a key step in the initiation of apoptosis.

Application of Recombinant Human VDAC2 Protein

Recombinant Human VDAC2 Protein has a wide range of applications in both basic research and biotechnology. Its function in regulating metabolite and ion transport makes it a valuable tool for studying mitochondrial metabolism and cellular signaling. By modulating the activity of VDAC2, researchers can gain insights into the role of this protein in various cellular processes.

In biotechnology, Recombinant Human VDAC2 Protein is used in drug discovery and development. Its involvement in apoptosis makes it a potential target for anti-cancer therapies. By studying the structure and function of VDAC2, researchers can identify potential compounds that can modulate its activity and potentially inhibit cancer cell growth.

Furthermore, Recombinant Human VDAC2 Protein is also used in the production of antibodies for diagnostic and therapeutic purposes. It is a common antigen used to generate specific antibodies that can be used to detect the presence of VDAC2 in various tissues and cell types. These antibodies can also be used to target VDAC2 in cancer cells, potentially leading to targeted therapies.

Conclusion

In summary, Recombinant Human VDAC2 Protein is a highly conserved transmembrane protein that plays important roles in regulating metabolite and ion transport, mitochondrial membrane potential, and apoptosis. Its structure and function have been extensively studied, and it has a wide range of applications in basic research and biotechnology. With its potential as a drug target and its use in antibody production, Recombinant Human VDAC2 Protein continues to be an important protein in the scientific community.