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Brand: ProteoGenix

Pertuzumab Biosimilar – Anti-ERBB2, EGFR2, CD340 mAb – Research Grade

Clonality:
Monoclonal Antibody
Isotype:
IgG1, kappa

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Pertuzumab Biosimilar - Anti-ERBB2, EGFR2, CD340 mAb binds to Human CD340/ERBB2/HER2 in indirect ELISA Assay
SDS-PAGE for Pertuzumab Biosimilar - Anti-ERBB2, EGFR2, CD340 mAb
Flow Cytometry results for Pertuzumab Biosimilar - Anti-ERBB2, EGFR2, CD340 mAb - Research Grade
Flow Cytometry results for Pertuzumab Biosimilar - Anti-ERBB2, EGFR2, CD340 mAb - Research Grade
Flow Cytometry results for Pertuzumab Biosimilar - Anti-ERBB2, EGFR2, CD340 mAb - Research Grade
Flow Cytometry results for Pertuzumab Biosimilar - Anti-ERBB2, EGFR2, CD340 mAb - Research Grade
Pertuzumab Biosimilar - Anti-ERBB2, EGFR2, CD340 mAb - Research Grade

Pertuzumab Biosimilar - Anti-ERBB2, EGFR2, CD340 mAb - Research Grade

Product name Pertuzumab Biosimilar - Anti-ERBB2, EGFR2, CD340 mAb - Research Grade
Uniprot ID P04626
Source CAS 380610-27-5
Species Humanized
Expression system Mammalian cells
Raw Antigen Sequence MELAALCRWGLLLALLPPGAASTQVCTGTDMKLRLPASPETHLDMLRHLYQGCQVVQGNLELTYLPTNASLSFLQDIQEVQGYVLIAHNQVRQVPLQRLRIVRGTQLFEDNYALAVLDNGDPLNNTTPVTGASPGGLRELQLRSLTEILKGGVLIQRNPQLCYQDTILWKDIFHKNNQLALTLIDTNRSRACHPCSPMCKGSRCWGESSEDCQSLTRTVCAGGCARCKGPLPTDCCHEQCAAGCTGPKHSDCLACLHFNHSGICELHCPALVTYNTDTFESMPNPEGRYTFGASCVTACPYNYLSTDVGSCTLVCPLHNQEVTAEDGTQRCEKCSKPCARVCYGLGMEHLREVRAVTSANIQEFAGCKKIFGSLAFLPESFDGDPASNTAPLQPEQLQVFETLEEITGYLYISAWPDSLPDLSVFQNLQVIRGRILHNGAYSLTLQGLGISWLGLRSLRELGSGLALIHHNTHLCFVHTVPWDQLFRNPHQALLHTANRPEDECVGEGLACHQLCARGHCWGPGPTQCVNCSQFLRGQECVEECRVLQGLPREYVNARHCLPCHPECQPQNGSVTCFGPEADQCVACAHYKDPPFCVARCPSGVKPDLSYMPIWKFPDEEGACQPCPINCTHSCVDLDDKGCPAEQRASPLTSIISAVVGILLVVVLGVVFGILIKRRQQKIRKYTMRRLLQETELVEPLTPSGAMPNQAQMRILKETELRKVKVLGSGAFGTVYKGIWIPDGENVKIPVAIKVLRENTSPKANKEILDEAYVMAGVGSPYVSRLLGICLTSTVQLVTQLMPYGCLLDHVRENRGRLGSQDLLNWCMQIAKGMSYLEDVRLVHRDLAARNVLVKSPNHVKITDFGLARLLDIDETEYHADGGKVPIKWMALESILRRRFTHQSDVWSYGVTVWELMTFGAKPYDGIPAREIPDLLEKGERLPQPPICTIDVYMIMVKCWMIDSECRPRFRELVSEFSRMARDPQRFVVIQNEDLGPASPLDSTFYRSLLEDDDMGDLVDAEEYLVPQQGFFCPDPAPGAGGMVHHRHRSSSTRSGGGDLTLGLEPSEEEAPRSPLAPSEGAGSDVFDGDLGMGAAKGLQSLPTHDPSPLQRYSEDPTVPLPSETDGYVAPLTCSPQPEYVNQPDVRPQPPSPREGPLPAARPAGATLERPKTLSPGKNGVVKDVFAFGGAVENPEYLTPQGGAAPQPHPPPAFSPAFDNLYYWDQDPPERGAPPSTFKGTPTAENPEYLGLDVPV
Purity >85%
Buffer PBS buffer PH7.5
Delivery condition Blue ice (+4°C)
Delivery lead time in business days 3-5 days if in stock; 3-5 weeks if production needed
Delivery Time 3-5 days if in stock; 3-5 weeks if production needed
Storage condition store at -80°C
Brand ProteoGenix
Applications ELISA,WB
Aliases /Synonyms Pertuzumab,OMNITARG,rhuMAB 2C4,ERBB2, EGFR2, CD340,anti-ERBB2, EGFR2, CD340
Reference PX-TA1058
Note For research use only. Not suitable for clinical or therapeutic use.
Isotype IgG1-kappa
Clonality Monoclonal Antibody
Product name Pertuzumab Biosimilar - Anti-ERBB2, EGFR2, CD340 mAb - Research Grade
Uniprot ID P04626
Source CAS 380610-27-5
Species Humanized
Expression system Mammalian cells
Raw Antigen Sequence MELAALCRWGLLLALLPPGAASTQVCTGTDMKLRLPASPETHLDMLRHLYQGCQVVQGNLELTYLPTNASLSFLQDIQEVQGYVLIAHNQVRQVPLQRLRIVRGTQLFEDNYALAVLDNGDPLNNTTPVTGASPGGLRELQLRSLTEILKGGVLIQRNPQLCYQDTILWKDIFHKNNQLALTLIDTNRSRACHPCSPMCKGSRCWGESSEDCQSLTRTVCAGGCARCKGPLPTDCCHEQCAAGCTGPKHSDCLACLHFNHSGICELHCPALVTYNTDTFESMPNPEGRYTFGASCVTACPYNYLSTDVGSCTLVCPLHNQEVTAEDGTQRCEKCSKPCARVCYGLGMEHLREVRAVTSANIQEFAGCKKIFGSLAFLPESFDGDPASNTAPLQPEQLQVFETLEEITGYLYISAWPDSLPDLSVFQNLQVIRGRILHNGAYSLTLQGLGISWLGLRSLRELGSGLALIHHNTHLCFVHTVPWDQLFRNPHQALLHTANRPEDECVGEGLACHQLCARGHCWGPGPTQCVNCSQFLRGQECVEECRVLQGLPREYVNARHCLPCHPECQPQNGSVTCFGPEADQCVACAHYKDPPFCVARCPSGVKPDLSYMPIWKFPDEEGACQPCPINCTHSCVDLDDKGCPAEQRASPLTSIISAVVGILLVVVLGVVFGILIKRRQQKIRKYTMRRLLQETELVEPLTPSGAMPNQAQMRILKETELRKVKVLGSGAFGTVYKGIWIPDGENVKIPVAIKVLRENTSPKANKEILDEAYVMAGVGSPYVSRLLGICLTSTVQLVTQLMPYGCLLDHVRENRGRLGSQDLLNWCMQIAKGMSYLEDVRLVHRDLAARNVLVKSPNHVKITDFGLARLLDIDETEYHADGGKVPIKWMALESILRRRFTHQSDVWSYGVTVWELMTFGAKPYDGIPAREIPDLLEKGERLPQPPICTIDVYMIMVKCWMIDSECRPRFRELVSEFSRMARDPQRFVVIQNEDLGPASPLDSTFYRSLLEDDDMGDLVDAEEYLVPQQGFFCPDPAPGAGGMVHHRHRSSSTRSGGGDLTLGLEPSEEEAPRSPLAPSEGAGSDVFDGDLGMGAAKGLQSLPTHDPSPLQRYSEDPTVPLPSETDGYVAPLTCSPQPEYVNQPDVRPQPPSPREGPLPAARPAGATLERPKTLSPGKNGVVKDVFAFGGAVENPEYLTPQGGAAPQPHPPPAFSPAFDNLYYWDQDPPERGAPPSTFKGTPTAENPEYLGLDVPV
Purity >85%
Buffer PBS buffer PH7.5
Delivery condition Blue ice (+4°C)
Delivery lead time in business days 3-5 days if in stock; 3-5 weeks if production needed
Delivery Time 3-5 days if in stock; 3-5 weeks if production needed
Storage condition store at -80°C
Brand ProteoGenix
Applications ELISA,WB,,,
Aliases /Synonyms Pertuzumab,OMNITARG,rhuMAB 2C4,ERBB2, EGFR2, CD340,anti-ERBB2, EGFR2, CD340
Reference PX-TA1058
Note For research use only. Not suitable for clinical or therapeutic use.
Isotype IgG1-kappa
Clonality Monoclonal Antibody
Product name PX-TA1058-50
Uniprot ID P04626
Source CAS 380610-27-5
Species Humanized
Expression system Mammalian cells
Raw Antigen Sequence MELAALCRWGLLLALLPPGAASTQVCTGTDMKLRLPASPETHLDMLRHLYQGCQVVQGNLELTYLPTNASLSFLQDIQEVQGYVLIAHNQVRQVPLQRLRIVRGTQLFEDNYALAVLDNGDPLNNTTPVTGASPGGLRELQLRSLTEILKGGVLIQRNPQLCYQDTILWKDIFHKNNQLALTLIDTNRSRACHPCSPMCKGSRCWGESSEDCQSLTRTVCAGGCARCKGPLPTDCCHEQCAAGCTGPKHSDCLACLHFNHSGICELHCPALVTYNTDTFESMPNPEGRYTFGASCVTACPYNYLSTDVGSCTLVCPLHNQEVTAEDGTQRCEKCSKPCARVCYGLGMEHLREVRAVTSANIQEFAGCKKIFGSLAFLPESFDGDPASNTAPLQPEQLQVFETLEEITGYLYISAWPDSLPDLSVFQNLQVIRGRILHNGAYSLTLQGLGISWLGLRSLRELGSGLALIHHNTHLCFVHTVPWDQLFRNPHQALLHTANRPEDECVGEGLACHQLCARGHCWGPGPTQCVNCSQFLRGQECVEECRVLQGLPREYVNARHCLPCHPECQPQNGSVTCFGPEADQCVACAHYKDPPFCVARCPSGVKPDLSYMPIWKFPDEEGACQPCPINCTHSCVDLDDKGCPAEQRASPLTSIISAVVGILLVVVLGVVFGILIKRRQQKIRKYTMRRLLQETELVEPLTPSGAMPNQAQMRILKETELRKVKVLGSGAFGTVYKGIWIPDGENVKIPVAIKVLRENTSPKANKEILDEAYVMAGVGSPYVSRLLGICLTSTVQLVTQLMPYGCLLDHVRENRGRLGSQDLLNWCMQIAKGMSYLEDVRLVHRDLAARNVLVKSPNHVKITDFGLARLLDIDETEYHADGGKVPIKWMALESILRRRFTHQSDVWSYGVTVWELMTFGAKPYDGIPAREIPDLLEKGERLPQPPICTIDVYMIMVKCWMIDSECRPRFRELVSEFSRMARDPQRFVVIQNEDLGPASPLDSTFYRSLLEDDDMGDLVDAEEYLVPQQGFFCPDPAPGAGGMVHHRHRSSSTRSGGGDLTLGLEPSEEEAPRSPLAPSEGAGSDVFDGDLGMGAAKGLQSLPTHDPSPLQRYSEDPTVPLPSETDGYVAPLTCSPQPEYVNQPDVRPQPPSPREGPLPAARPAGATLERPKTLSPGKNGVVKDVFAFGGAVENPEYLTPQGGAAPQPHPPPAFSPAFDNLYYWDQDPPERGAPPSTFKGTPTAENPEYLGLDVPV
Purity >85%
Buffer PBS buffer PH7.5
Delivery condition Blue ice (+4°C)
Delivery lead time in business days 3-5 days if in stock; 3-5 weeks if production needed
Delivery Time 3-5 days if in stock; 3-5 weeks if production needed
Storage condition store at -80°C
Brand ProteoGenix
Aliases /Synonyms Pertuzumab,OMNITARG,rhuMAB 2C4,ERBB2, EGFR2, CD340,anti-ERBB2, EGFR2, CD340
Reference PX-TA1058
Note For research use only. Not suitable for human use.
Isotype IgG1, kappa
Clonality Monoclonal Antibody

General information on Anti-ERBB2/EGFR2/CD340(Homo sapiens) (Pertuzumab) Monoclonal Antibody

Pertuzumab is a recombinant humanized monoclonal antibody that targets the extracellular dimerization domain (subdomain II) of human epidermal growth factor receptor 2 protein (HER2). It was first used with docetaxel and another HER2-targeting monoclonal antibody trastuzumab to treat metastatic HER2-positive breast cancer. Since then, its indications have been expanded to be used as both neoadjuvant therapy and adjuvant therapy for the treatment of HER2-positive breast cancer with a high risk of recurrence.

Pertuzumab Biosimilar - Anti-ERBB2, EGFR2, CD340 mAb binds to Human CD340/ERBB2/HER2 in indirect ELISA Assay

Pertuzumab Biosimilar - Anti-ERBB2, EGFR2, CD340 mAb binds to Human CD340/ERBB2/HER2 in indirect ELISA Assay

Immobilized CD340 Recombinant Protein (cat. No.PX-P4121) at 0.5µg/mL (100µL/well) can bind Pertuzumab Biosimilar - Anti-ERBB2, EGFR2, CD340 mAb (cat. No.PX-TA1058) in indirect ELISA with Goat Anti-Human IgG secondary antibody coupled with HRP measured by OD450 giving an EC50 at 9.566M.

SDS-PAGE for Pertuzumab Biosimilar - Anti-ERBB2, EGFR2, CD340 mAb

SDS-PAGE for Pertuzumab Biosimilar - Anti-ERBB2, EGFR2, CD340 mAb

Pertuzumab Biosimilar - Anti-ERBB2, EGFR2, CD340 mAb, on SDS-PAGE under reducing and non-reducing condition. The gel was stained overnight with Coomassie Blue. The purity of the antibody is greater than 95%.

Flow Cytometry results for Pertuzumab Biosimilar - Anti-ERBB2, EGFR2, CD340 mAb - Research Grade

Flow Cytometry results for Pertuzumab Biosimilar - Anti-ERBB2, EGFR2, CD340 mAb - Research Grade

Flow-cytometry using anti-human CD340 antibody. MCF-7 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD340 monoclonal antibody (Catalog PX-TA1058, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: PTX18862) and cells analysed on a NovoCyte Flow Cytometer.

Flow Cytometry results for Pertuzumab Biosimilar - Anti-ERBB2, EGFR2, CD340 mAb - Research Grade

Flow Cytometry results for Pertuzumab Biosimilar - Anti-ERBB2, EGFR2, CD340 mAb - Research Grade

Target Cells were stained with an irrelevant antibody or Pertuzumab Biosimilar - Anti-ERBB2, EGFR2, CD340 mAb - Research Grade at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Goat Anti-Human IgG Polyclonal Antibody, FITC (PTX18862) and cells analysed on a NovoCyte Flow Cytometer.

Flow Cytometry results for Pertuzumab Biosimilar - Anti-ERBB2, EGFR2, CD340 mAb - Research Grade

Flow Cytometry results for Pertuzumab Biosimilar - Anti-ERBB2, EGFR2, CD340 mAb - Research Grade

Flow-cytometry using anti-human CD340 antibody. MDA-MB-231 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD340 monoclonal antibody (Catalog PX-TA1058, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: PTX18862) and cells analysed on a NovoCyte Flow Cytometer.

Flow Cytometry results for Pertuzumab Biosimilar - Anti-ERBB2, EGFR2, CD340 mAb - Research Grade

Flow Cytometry results for Pertuzumab Biosimilar - Anti-ERBB2, EGFR2, CD340 mAb - Research Grade

Target Cells were stained with an irrelevant antibody or Pertuzumab Biosimilar - Anti-ERBB2, EGFR2, CD340 mAb - Research Grade at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Goat Anti-Human IgG Polyclonal Antibody, FITC (PTX18862) and cells analysed on a NovoCyte Flow Cytometer.

Pertuzumab Biosimilar - Anti-ERBB2, EGFR2, CD340 mAb - Research Grade

  • Rocha Tapia A, Abgottspon F, Nilvebrant J, Nygren P-Å, Duclos Ivetich S, Bello Hernandez AJ, et al. Site-directed Conjugation of Single-Stranded DNA to Affinity Proteins: Quantifying the Importance of Conjugation Strategy. ChemRxiv. 2023; doi:https://doi.org/10.26434/chemrxiv-2023-hxx06

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