Lymphoprep™ should be stored at room temperature. It should be kept sterile and protected from light. Under these conditions Lymphoprep™ can be used for up to 3 years. Prolonged exposure to direct sunlight leads to release of iodine from the sodium diatrizoate molecule. This effect is negligible when working with this solution on a day to day basis.
Brand
Axis-Shield/Serumwerk
Composition
The solution contains sodium diatrizoate and polysaccharide in the following concentrations: Sodium Diatrizoate 9.1% (w/v) Polysaccharide 5.7% (w/v)
Principle of the separation procedure
Prior to Lymphoprep, the most common technique for separating leucocytes is to mix blood with a compound which aggregates the erythrocytes, thereby increasing their sedimentation rate. The sedimentation of leucocytes is only slightly affected and can be collected from the upper part of the tube when the erythrocytes have settled. Using a mixture of sodium metrizoate and polysaccharide – initial version of Lymphoprep™ - Bøyum (1968) developed a one-step centrifugal technique for isolation of lymphocytes. Thorsby and Bratlie (1970) used this technique with only slight modifications in the preparation of pure lymphocyte suspension for cytotoxicity tests and lymphocyte cultures. As emphasized also by other authors, Harris and Ukayiofo (1969), Ting and Morris (1971), Lymphoprep™ is a reliable, simple and quick method suitable for the preparation of lymphocyte preparations from cadaver blood, and from anticoagulated blood stored at room temperature for up to 6 hours.
Advantages
Lymphoprep™ vs Ficoll-Paque & Histopaque 1077
• Low endoxin • Less expensive • Lymphoprep™ is the only product of its kind produced under GMP conditions • Can be a good substitute for Ficoll-Paque™ with no further change of the existing protocols
Alternatives
Lymphoprep™ can be a good substitution to:
Ficoll® Ficoll-Paque Histopaque Buffy booster Cell separation media
Reference
1114544
Description of Lymphoprep™
About Lymphoprep™
• Lymphoprep™ is a ready-made, sterile and endotoxin-tested density gradient medium used to isolate mononuclear cells issued from cord blood, peripheral blood and bone marrow based on their cell density.
• Granulocytes and erythrocytes sediment through the Lymphoprep™ layer during centrifugation because they have a higher density than mononuclear cells.
• Lymphoprep™ can be used for the preparation of pure lymphocyte suspensions for tissue typing, antilymphocyte sera and immunological research.
• The use of Lymphoprep™ has been proven to be quick, simple, reliable, and has good outcomes with blood samples from most regular individuals and patients.
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