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Flow-cytometry using anti-human CD47 antibody. Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD47 monoclonal antibody (Catalog ARO-A15839, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: PTX18862) and cells analysed on a NovoCyte Flow Cytometer.
Mouse CD47 recombinant protein(cat. No.PX-P6437) at 0.5µg/mL (100µL/well) can bind Human CD47/MER6 Monoclonal Antibody in indirect ELISA with Goat secondary antibody measured by OD450.
PTX17887
KPTX243
PX-P6437
PTX19763
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