Human CD326/EPCAM HEK293T Stable Cell Line

Introduction to Human CD326/EPCAM HEK293T Stable Cell Line

Human CD326/EPCAM HEK293T Stable Cell Line is a valuable tool for researchers studying the role of CD326/EPCAM in various biological processes and diseases. This stable cell line is derived from HEK293T cells, a popular human embryonic kidney cell line that is commonly used in research due to its ease of transfection and high transfection efficiency. CD326/EPCAM, also known as epithelial cell adhesion molecule, is a transmembrane glycoprotein that plays a critical role in cell adhesion and signaling. It is expressed in a variety of tissues, including epithelial cells, and has been implicated in various cellular processes such as cell proliferation, differentiation, and migration.

Structure of Human CD326/EPCAM

Human CD326/EPCAM is a type I transmembrane glycoprotein with a molecular weight of approximately 40 kDa. It consists of an extracellular domain, a transmembrane domain, and a short cytoplasmic tail. The extracellular domain contains two epidermal growth factor (EGF)-like repeats and two thyroglobulin type-1 (Tg1) domains, which are involved in protein-protein interactions and cell adhesion. The transmembrane domain anchors the protein to the cell membrane, while the cytoplasmic tail contains potential phosphorylation sites that may regulate its function.

Activity of Human CD326/EPCAM

The main function of CD326/EPCAM is to mediate cell-cell adhesion through homophilic interactions. This means that CD326/EPCAM molecules on adjacent cells bind to each other, forming strong adhesions that are important for maintaining tissue integrity and barrier function. In addition to its role in cell adhesion, CD326/EPCAM has been shown to play a role in cell signaling. It has been reported to activate the Wnt/β-catenin signaling pathway, which is involved in cell proliferation and differentiation. CD326/EPCAM has also been implicated in the regulation of cell migration and invasion, making it a potential therapeutic target for cancer metastasis.

Application of Human CD326/EPCAM HEK293T Stable Cell Line

The Human CD326/EPCAM HEK293T Stable Cell Line offers a reliable and convenient system for studying the function of CD326/EPCAM in various biological processes. This stable cell line can be used in a wide range of applications, including flow cytometry, immunofluorescence, and Western blotting. The stable expression of CD326/EPCAM in these cells allows for consistent and reproducible results, making it an ideal tool for research and drug discovery.

Flow Cytometry

The Human CD326/EPCAM HEK293T Stable Cell Line can be used in flow cytometry to analyze the expression of CD326/EPCAM on the cell surface. This technique allows for the quantification of CD326/EPCAM expression levels and can also be used to identify subpopulations of cells expressing different levels of CD326/EPCAM. This is particularly useful in cancer research, where CD326/EPCAM is often overexpressed in tumor cells.

Immunofluorescence

The stable expression of CD326/EPCAM in HEK293T cells allows for the visualization of CD326/EPCAM localization and distribution using immunofluorescence. This technique involves labeling CD326/EPCAM with a fluorescent antibody, which can then be visualized under a fluorescence microscope. This allows for the examination of CD326/EPCAM expression in different cell types and tissues, providing valuable insights into its role in various biological processes.