Human CD194/CCR4 HEK293T Stable Cell Line

Title: Introduction to Human CD194/CCR4 HEK293T Stable Cell Line: Structure, Activity and Application

Human CD194/CCR4 HEK293T Stable Cell Line: An Overview

Human CD194, also known as CC chemokine receptor 4 (CCR4), is a G protein-coupled receptor that plays a critical role in the immune system. It is highly expressed on T cells, particularly on Th2 cells, and is involved in the recruitment and activation of these cells at sites of inflammation. Dysregulation of CCR4 has been linked to various diseases, making it an attractive therapeutic target. In this article, we will discuss the structure, activity, and application of Human CD194/CCR4 HEK293T Stable Cell Line.

Structure of Human CD194/CCR4

Human CD194/CCR4 is a seven-transmembrane domain receptor that belongs to the G protein-coupled receptor superfamily. It is composed of 360 amino acids and has a molecular weight of approximately 40 kDa. The receptor has three extracellular loops, three intracellular loops, and an N-terminal and C-terminal domain. The extracellular loops are involved in ligand binding, while the intracellular loops interact with G proteins to initiate downstream signaling pathways.

Activity of Human CD194/CCR4

Human CD194/CCR4 is activated by the binding of its ligands, CCL17 and CCL22, which are members of the CC chemokine family. Upon ligand binding, the receptor undergoes conformational changes that lead to the recruitment and activation of G proteins. This, in turn, triggers a cascade of signaling events, including the activation of phospholipase C, which results in the production of inositol triphosphate and diacylglycerol. These signaling pathways ultimately lead to the activation of transcription factors and the expression of genes involved in cell migration and inflammation.

Application of Human CD194/CCR4 HEK293T Stable Cell Line

The Human CD194/CCR4 HEK293T Stable Cell Line is a powerful tool for studying the structure and function of CCR4 and its role in various diseases. This cell line is stably transfected with the human CD194 gene and expresses high levels of functional CCR4 on its surface. It can be used for a variety of applications, including flow cytometry, ligand binding assays, and downstream signaling studies.

Flow Cytometry: The Human CD194/CCR4 HEK293T Stable Cell Line can be used for flow cytometry analysis to assess the expression of CCR4 on the surface of cells. This technique allows for the detection and quantification of CCR4 expression in various cell types, providing valuable insights into its role in different diseases.

Ligand Binding Assays: The stable expression of functional CCR4 on the Human CD194/CCR4 HEK293T Stable Cell Line makes it an ideal tool for ligand binding assays. This cell line can be used to determine the affinity and specificity of CCR4 for its ligands, as well as to screen for potential CCR4 antagonists or agonists.

Downstream Signaling Studies: The Human CD194/CCR4 HEK293T Stable Cell Line can also be used for downstream signaling studies to investigate the intracellular signaling pathways activated by CCR4. This can provide valuable insights into the mechanisms underlying CCR4-mediated immune responses and identify potential therapeutic targets.

Therapeutic Target: CCR4 has been implicated in various diseases, including asthma, atopic dermatitis, and cancer. The Human CD194/CCR4 HEK293T Stable Cell Line can be used to study the role of CCR4 in these diseases and identify potential therapeutic targets for the development of novel treatments.

In conclusion, the Human CD194/CCR4 HEK293T Stable Cell Line is a valuable tool for studying the structure, activity, and function of CCR4. Its stable expression of functional CCR4 makes it an ideal model for various applications, including flow cytometry, ligand binding assays, and downstream signaling studies.