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Picomolar-affinity antibodies with guaranteed performance in your application
Accelerate antibody discovery with high-throughput Single B Cell screening while preserving natural VH/VL pairing.
Single B Cell antibody technology enables the isolation and screening of individual antigen-specific B cells directly from the immune repertoire. It preserves natural VH/VL pairing, enabling rapid recovery of sequence-defined monoclonal antibody candidates.
High-throughput screening
Up to 960 cells/run
Antigen-specific single B cells isolated for downstream screening and characterization.
Application relevance
11 applications
Antibodies validated in the assay relevant to your project.
Native antibody biology
Natural VH/VL pairing
Preserve the native heavy- and light-chain combination of each selected B cell.
What is your project focused on?
Start with the assay you need the antibody to perform in. Our workflow is designed to identify binders and validate them against the experimental requirements of your project—not only against the antigen.
Your antibody is tested in the assay that matters to you. You receive antibodies guaranteed to work in your specific application.
Standard ELISA
Sandwich ELISA
Flow cytometry (FACS)
Immunohistofluorescence
Immunofluorescence
Lateral flow assay (LFA)
Competitive ELISA
Immunoprecipitation
Modification-specific detection
Western Blot
Immunohistochemistry
Planning a diagnostic or research project? Tell us your target, antigen format and final assay. If you need recognition of a native membrane target, discrimination between modified and unmodified antigen, cross-reactivity control, or a matched antibody pair, include this in your request so our scientists can adapt the screening strategy from the beginning.
Test the antibodies in your own laboratory, under your real experimental conditions, before accepting the final delivery. You only pay in full for antibodies that perform in your application.
ProteoGenix validation
We test the antibodies in their final application.
Your lab test
You test the antibodies under your own experimental conditions.
Pay If Satisfied
You only pay the full project fee if you are satisfied with their performance.
Rabbit Single B Cell screening
Rabbit immune repertoires offer high antibody diversity, picomolar-range affinities, and broad epitope coverage, making them particularly valuable for demanding diagnostic applications. Single B Cell screening enables direct selection of the best candidates from this repertoire.
Therapeutic Single B Cell discovery
Generate fully human, functionally relevant antibody candidates and move faster from Single B Cell screening to therapeutic development.
For therapeutic programmes, IP-free humanized mice generate fully human antibodies, eliminating the humanization step entirely and delivering sequences with full freedom to operate from day one.
IP-free humanized mice
Fully human antibodies
Generate fully human antibody sequences directly from immunized humanized mice.
RocketAbs™ fast-track option
45-day immunization
Accelerate the immunization stage and move faster into Single B Cell screening and candidate selection.
Functional candidate selection
Our optional add-ons screening protocols can complement the standard single B-cell service, ensuring performance for your specific project.
Neutralization
NeutAb protocol
Competitive ELISA screening to identify clones with blocking or neutralizing activity against the active site of your target
Selectivity
Counter-screening protocol
Negative ELISA screening against a counter-antigen to confirm selectivity and exclude cross-reactive clones
Cross-reactivity
Cross-reactivity protocol
Positive/negative ELISA screening against a second antigen (protein, peptide, or small molecule) to profile selectivity or confirm cross-species binding
Not sure which screening strategy fits your programme?
Share your target, intended mechanism of action and selectivity requirements with our scientists.
One integrated workflow connects immunization, antigen-specific B-cell isolation, functional screening, VH/VL sequence recovery and recombinant antibody production.
High-Throughput B-Cell Isolation
Up to 960 antigen-specific B cells are isolated per run using high-resolution FACS, with each cell individually sorted and cultured.
Functional Validation & Antigen Positivity Confirmation
Each sorted B cell is monitored and tested to confirm antigen-specific binding, ensuring only truly positive clones move forward.
Ranking & Top Candidate Selection
Validated clones are ranked by performance. The top 10 highest-performing B-cell clones are selected for downstream processing.
VH/VL Sequence Recovery
Heavy and light chain variable sequences are recovered from the top clones using a validated amplification workflow that preserves natural VH/VL pairing.
Recombinant Antibody Production
Selected sequences are expressed in ProteoGenix’s CHO platform to produce recombinant antibodies at scale.
QC & Functional Validation
Produced antibodies undergo quality control and functional validation to confirm performance and suitability for the project’s application.
What determines the exact workflow? Antigen format, immune response, required specificity, final assay, desired cross-reactivity and whether you need binding-only or functional screening. Contact us with these parameters and our scientists can recommend the appropriate project design.
Designing your Single B Cell project
The quality of a Single B Cell screening campaign starts with the immunization strategy. ProteoGenix supports several species and antigen formats so antigen presentation can be adapted to the biology of your target and to the antibody profile you need to recover.
Standard Single B Cell programmes are available in mouse, rabbit and IP-free humanized mice. The page also supports work from suitable client-provided samples depending on project feasibility.
Mouse
Rabbit
IP-free humanized mice
Recombinant proteins
Including challenging targets such as membrane proteins (GPCRs, ion channels), multi-domain complexes, and low-immunogenicity antigens.
Peptides
Ideal for high specificity, PTM detection, or when the protein is unavailable.
Post-translational modifications
Phosphorylation, acetylation, methylation… with modified vs. unmodified antibody validation.
Haptens & small molecules
Conjugated to KLH or BSA. From pesticides, steroids and toxins to lipids, nucleotides, amino acids and saccharides…
Whole cells & VLPs
Cell-based immunization and panning for membrane proteins in native conformation.
DNA constructs
Useful when the protein is hard to produce. Combines advantages of protein and peptide immunization.
RocketAbs™ immunization protocol
Our proprietary RocketAbs™ immunization protocol is designed to generate robust, high-titer immune responses on accelerated timelines while reducing antigen consumption.
When can RocketAbs™ be particularly useful?
Single B cell screening is the starting point, not the finish line. Every service below is available as a seamless add-on to your discovery project.
Structure-aware AI variant design combined with wet-lab validation.
Developability · Affinity maturation · Humanization
Convert your hit into any therapeutic format: IgG, Fab, scFv-Fc, VHH-Fc, bispecific, ADC…, with Fc engineering for effector function modulation.
Extensive IP-free portfolio
Recombinant antibody production
From µg to g and stable cell line development. All formats: IgG, Fab, VHH, scFv…
Powered by XtenCHO™ Race
Data Reports
Review experimental examples to see how antigen format, immunization, FACS sorting, screening and recombinant antibody production are connected in actual Single B Cell projects.
Peptide antigen
Recombinant protein antigen
They trusted us
“I was immediately impressed by the speed of the response and initial virtual meeting where it was clear from the questions I was being asked and the pragmatic discussion that ProteoGenix’s staff knew the biology very well and had extensive experience in this area and these weren’t salespeople but scientists.”
“We wouldn’t hesitate to recommend ProteoGenix to any lab – especially labs that have a challenging project where a collaboration rather than a simple service agreement is likely to be far more productive.”
Richard Beatson
Honorary Associate Professor
ProteoGenix partnered with MicrosensDx to develop antibodies for a novel sepsis diagnostic approach. Using our Single B-cell screening platform, we generated antibodies capable of specifically recognizing the oxidized form of a novel biomarker.
“ProteoGenix is a logical partner for us, sharing our belief in the need to radically improve sepsis diagnosis and outcomes for patients and their families. The company’s antibody expertise and technical knowhow will be essential in ensuring consistent assay performance as we move towards clinical testing.”
Simon Walker,
CEO of MicrosensDx
Explore our antibody discovery services and alternative platforms, or contact our scientists to discuss the most appropriate approach.
