Standard ELISA package: ATX-PACK1M

  • Mice injections
  • Immune response control by ELISA
  • Selection of the best mice for fusion
  • Screening of the fusion product by ELISA : around 10 x 96-well plates
  • Subcloning of 2 parental clones (2-3 rounds of limiting dilutions) examined by ELISA
  • If we succeed in producing an antibody that works in ELISA against the immunogen, we charge the full package price
  • If we don’t, we charge EUR 800
  • 10 -20 best clones at parental stage for customer’s selection
  • One hybridoma cell line (four frozen vials)
  • 5 ml culture supernatant
  • Injections : 8-12 weeks
  • Fusion screening:3-4 weeks
  • Subcloning: 8-12 weeks
  • TOTAL : 19-28 days

Premium ELISA guaranteed package: ATX-PACK2M

  • Antigen design (design of the most appropriate fragment to be produced)
  • Two gene syntheses (each gene optimized for a specific expression system) and 2 subclonings
  • Expression tests in two systems taken from E. coli, B. Subtilis, P. Pastoris, insect cells and mammalian cells. To be determined by ProteoGenix.
  • Production of the antigen in the most appropriate expression system, depending on the yield and the project requirements.
  • Mice injections
  • Immune response control by ELISA
  • Selection of the two best mice for two fusions, in order to have a large number and variety of clones
  • Screening of the fusion product by ELISA: around 10 x 96-well plates
  • Subcloning of two parental clones (2-3 rounds of limiting dilutions) examined by ELISA
  • Production and purification of 2mg from the best clone
  • Level 1: If we don’t succeed in producing an antibody that works in ELISA against the immunogen, we charge EUR 1000
  • Level 2 : If we succeed in producing an antibody that works in ELISA against the immunogen, we charge only a small part of the cost
  • Level 3 : If after testing the purified antibody the customer wants to receive the hybridoma, we charge the full package price
  • 10 -20 best clones at parental stage for customer’s selection
  • 100 µg of purified antibody from the best clone to confirm hybridoma purchase
  • 50 µg of purified protein
  After total payment:  
  • 1.9 mg of purified antibody
  • One hybridoma cell line (four frozen vials)
  • 150 µg of purified protein
  • Both genes in pUC57
  • Gene synthesis: 3-4 weeks
  • Protein production : 7-9 weeks
  • Injections: 8-12 weeks
  • Fusion screening:4-5 weeks
  • Subcloning: 8-12 weeks
  • Antibody production: 3-4 weeks
  • Total: 33-46 weeks

Peptide guaranteed package: ATX-PACK8M

  • Two or three peptides: design, synthesis, and conjugation to KLH
  • Mice injections with a mix of the peptides
  • Immune response control by ELISA
  • Selection of the best mice for fusion
  • Screening of the fusion product by ELISA : around 10 x 96-well plates
  • Subcloning of two parental clones (2-3 rounds of limiting dilutions) examined by Elisa
  • If we don’t succeed in producing an antibody that works in ELISA against the peptides, we charge EUR 800
  • If we succeed, we charge the full package price
  • 10 -20 best clones at parental stage for customer’s selection
  • One hybridoma cell line (four frozen vials)
  • 2 mg of purified antibody
  • Peptide synthesis and conjugation : 3-4 weeks
  • Injections: 8-12 weeks
  • Fusion screening: 3-4 weeks
  • Subcloning: 8-12 weeks
  • Antibody production: 3-4 weeks
  • Total: 25-36 weeks

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