What sample formats can you sequence: hybridoma cells, supernatants, purified antibodies, or all of the above?

All of the above. We can sequence from hybridoma cells, culture supernatants, or purified antibodies.

How many cells or how much antibody do you need to guarantee high-quality sequencing?

We recommend 3 frozen vials, each containing 2–3 × 10⁶ cells/vial, to ensure optimal sequencing quality.

Can you handle low-producing or unstable hybridomas?

Yes. We routinely work on low-producing and unstable hybridomas, including complex cases.

Do you perform sequencing directly from the hybridoma’s mRNA or via de novo protein sequencing?

Both options are available. We can sequence from mRNA or perform de novo protein sequencing when needed.

Can you guarantee recovery of the correct functional antibody sequence?

Yes. Sequence recovery is guaranteed, if we don’t deliver your functional sequence, there is no fee.

Can you clone the antibody sequence into expression vectors after sequencing?

Yes, we can clone the recovered sequences into expression vectors upon request.

Do you offer optional humanization, affinity maturation, or expression optimization once the sequence is obtained?

Yes. We offer humanization, affinity maturation, expression optimization, and other downstream services.

Are you able to deliver a functional recombinant antibody based on the sequence you recover?

Yes. We can produce and deliver up to 2 mg of your recombinant antibody.

How long does the sequencing process take from start to finish?

Typical turnaround is about 1 week from project start.

What exactly is included in the final report?

You receive a full report with nucleotide and amino acid sequences, CDR and framework identification, plus expert guidance from a dedicated PhD scientist.

Antibody Sequencing Service

Your guaranteed antibody sequence delivered in 1 week

Week delivery starting time

0€

No sequence no fee

Years of experience

From protecting your antibody discovery through patent applications to enabling AI-driven engineering, antibody sequencing is a critical milestone in any development program. ProteoGenix provides this service at competitive pricing with rapid turnaround, while ensuring long-term preservation of your hybridoma-derived sequence. Securing the sequence creates a durable reference, allowing recombinant antibody production to continue even if the original hybridoma cell line is lost.

A Modular Sequencing service Built around Your Project

Hybridoma sequencing service
- No win, no fee
- 1 week starting time
- Competitive pricing
- Antibody variable domains (VH, VL) or full-length antibody (VH, VL, Fc)
Optional add-ons
- Gene synthesis with codon optimization for a chosen expression system: yeast, insect, or mammalian
- Recombinant antibody production
- Interaction test against soluble antigen or cell antigen (ELISA, FACS, KD determination by Biacore / SPRi / Octet…)
Comprehensive Deliverables
- A complete report including the CDR sequence annotated
- Plasmid containing the sequence (upon request)
- Up to 2mg recombinant antibody (upon request).