Recombinant Human P2RX6 Protein, N-His

Description of Recombinant Human P2RX6 Protein, N-His

Introduction

The Recombinant Human P2RX6 Protein is a type of recombinant protein that has been produced through genetic engineering techniques. It is a member of the P2X family of purinergic receptors, which are ion channels that are activated by extracellular ATP. The P2RX6 protein is primarily found in the central nervous system and plays a role in various physiological processes such as neurotransmission, inflammation, and pain sensation.

Structure of Recombinant Human P2RX6 Protein

The P2RX6 protein is composed of 489 amino acids and has a predicted molecular weight of approximately 55 kDa. It consists of two transmembrane domains, a large extracellular loop, and intracellular N- and C-termini. The extracellular loop contains several conserved regions that are important for ATP binding and channel activation. The intracellular termini are responsible for protein-protein interactions and regulation of channel activity.

Activity of Recombinant Human P2RX6 Protein

The P2RX6 protein functions as a ligand-gated ion channel, meaning it opens in response to the binding of ATP. Upon activation, the channel allows the influx of cations such as calcium, sodium, and potassium into the cell. This influx of ions leads to changes in membrane potential and triggers downstream signaling pathways. The P2RX6 protein is also involved in the release of neurotransmitters, such as glutamate and GABA, from neurons.

Studies have shown that the activity of the P2RX6 protein is modulated by various factors, including pH, divalent cations, and allosteric modulators. It has been suggested that these modulators play a role in fine-tuning the function of P2RX6 in different physiological conditions.

Application of Recombinant Human P2RX6 Protein

The recombinant human P2RX6 protein has a wide range of applications in both basic research and drug discovery. It is commonly used as an antigen in antibody production, as well as in functional assays to study the role of P2RX6 in different cellular processes. The recombinant protein can also be used in drug screening assays to identify potential modulators of P2RX6 activity.

One potential therapeutic application of the P2RX6 protein is in the treatment of pain. Studies have shown that P2RX6 is involved in the transmission of pain signals in the spinal cord, and targeting this protein may provide a new avenue for pain relief. Additionally, P2RX6 has been implicated in various neurological disorders, such as epilepsy and Alzheimer’s disease, making it a potential target for drug development in these areas.

Conclusion

The Recombinant Human P2RX6 Protein is a vital tool for studying the function and regulation of P2RX6 in various physiological processes. Its structure, activity, and potential applications make it a valuable resource for both basic research and drug discovery. Further studies on this protein may lead to a better understanding of its role in health and disease, and potentially pave the way for the development of new therapeutics targeting P2RX6.