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Anti-Human CFH Monoclonal Antibody, SAA0527, on SDS-PAGE. The gel was stained overnight with Coomassie Blue. The purity of the antibody is greater than 95%.
Target Cells were stained with an irrelevant antibody or Anti-Human CFH Monoclonal Antibody, SAA0527 at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Goat Anti-Human IgG Polyclonal Antibody, FITC (PTX18862) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry using anti-human CFH antibody.HepG2 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CFH antibody monoclonal antibody (Catalog # PTX19299 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # PTX17818) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry using FITC anti-human CFH antibody. HepG-2 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CFH monoclonal antibody (Catalog: PTX19299, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry using APC anti-human CFH antibody. HepG-2 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CFH monoclonal antibody (Catalog: PTX19299, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
PTX17816
PTX17818
ARO-A14604
ARO-A14609
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