Enzyme-Linked Immunosorbent Assay (ELISA) is a biochemical technique used to detect and quantify proteins, antibodies, antigens, and other molecules in a sample. ELISA is widely used in research and clinical…
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Enzyme-Linked Immunosorbent Assay (ELISA) is a biochemical technique used to detect and quantify proteins, antibodies, antigens, and other molecules in a sample. ELISA is widely used in research and clinical diagnostics to measure the presence and amount of a target molecule in a sample.
Principles of ELISA
In ELISA, a sample is incubated with a specific antibody that binds to the target molecule. The antibody is bound to an enzyme, such as horseradish peroxidase or alkaline phosphatase, which catalyzes a reaction that produces a colored product. The amount of the colored product is then measured and used to calculate the amount of target molecule in the sample.
How to choose Antibodies for ELISA?
When selecting antibodies for ELISA, it is important to consider the affinity of the antibody for the target molecule. High affinity antibodies will bind more tightly to the target molecule, resulting in more accurate results. In addition, it is important to consider the specificity of the antibody, as cross-reactivity with other molecules can lead to inaccurate results.
ProteoGenix Antibodies
ProteoGenix is a leading provider of antibodies for ELISA. The company offers a wide range of antibodies with high affinity and specificity for a variety of target molecules. In addition, ProteoGenix offers custom antibody production services, allowing researchers to create antibodies tailored to their specific needs.
