Baculovirus protein expression service

The baculovirus expression system is suitable for a wide range of proteins, particularly those requiring proper folding or post-translational modifications (PTMs) such as glycosylation and phosphorylation.

We work with both Spodoptera frugiperda and Trichoplusia ni cell lines. The choice of cell line is optimized based on your specific protein and project requirements.

Insect cells combine the best of both worlds: the post-translational modification machinery of eukaryotic systems — including glycosylation and phosphorylation patterns close to those of mammalian cells — with significantly lower production costs than mammalian expression systems. This makes them an ideal choice for producing high-quality proteins at a competitive price.

Yes. We handle the full virus generation workflow, from bacmid production to P1 and P2 virus stock amplification and titer determination, so you don’t need to provide any viral material to get started.

Yes, both are available as optional add-ons. We can design and synthesize your gene with codon optimization tailored for insect cell expression, and subclone it directly into the appropriate baculovirus expression vector.

Yes. Once expression conditions have been optimized at small scale, we can scale production up to 20L bioreactors to meet your quantity requirements.

Quality is assessed at multiple stages throughout the process. Expression is monitored by SDS-PAGE and Western Blot where needed. Purified protein can be further analyzed by analytical size exclusion chromatography (SEC) to assess homogeneity, and an optional polishing step is available to remove process-related contaminants including residual DNA, host cell proteins (HCP), and endotoxins.

Standard projects are completed within 4 to 6 weeks. Timelines may vary depending on the complexity of your protein and any additional service options selected, such as gene synthesis or large-scale production.

Simply reach out via our contact form or book a call directly with one of our PhD project managers. We’ll get back to you promptly to discuss your needs and provide a free tailored quote.