Recombinant Human LIN28A Protein, N-His-SUMO & C-Strep

Introduction to Recombinant Human LIN28A Protein

Recombinant Human LIN28A Protein, also known as LIN28A or LIN28, is a highly conserved RNA-binding protein that plays a crucial role in regulating gene expression and development. It is encoded by the LIN28A gene and is found in various tissues and cell types, including embryonic stem cells, fetal tissues, and some adult tissues.

Structure of Recombinant Human LIN28A Protein

Recombinant Human LIN28A Protein is a 25 kDa protein that consists of 209 amino acids. It has a unique structure with a cold shock domain (CSD) at the N-terminus and two CCHC zinc finger motifs at the C-terminus. The CSD is responsible for RNA binding, while the zinc finger motifs are involved in protein-protein interactions.

The structure of Recombinant Human LIN28A Protein is highly conserved across species, with 100% sequence identity between humans and mice. This indicates the importance of this protein in regulating gene expression and development.

Activity of Recombinant Human LIN28A Protein

Recombinant Human LIN28A Protein is a multifunctional protein that has been shown to have various activities in different cellular processes. Its main function is to bind to and regulate the expression of specific mRNAs, leading to the suppression of their translation. This regulation is achieved through the binding of LIN28A to the 3′ untranslated region (UTR) of target mRNAs.

In addition to its role in regulating gene expression, Recombinant Human LIN28A Protein has also been found to play a role in cell proliferation, differentiation, and metabolism. It has been shown to interact with various proteins and signaling pathways, such as the let-7 microRNA family, the PI3K/AKT/mTOR pathway, and the Wnt/β-catenin pathway, to regulate these cellular processes.

Application of Recombinant Human LIN28A Protein

Due to its diverse activities, Recombinant Human LIN28A Protein has been studied extensively in various fields, including developmental biology, stem cell research, and cancer biology. Its role in regulating gene expression and cell proliferation makes it a potential target for therapeutic interventions.

One of the major applications of Recombinant Human LIN28A Protein is in stem cell research. LIN28A is highly expressed in embryonic stem cells and has been shown to promote their self-renewal and pluripotency. This has led to the development of protocols for the differentiation of induced pluripotent stem cells (iPSCs) into specific cell types using LIN28A.

Recombinant Human LIN28A Protein has also been studied in the context of cancer biology. It is overexpressed in various types of cancers, and its overexpression has been associated with poor prognosis. This has led to the development of LIN28A inhibitors as potential anti-cancer therapies.

Furthermore, Recombinant Human LIN28A Protein has been used in a variety of research studies to investigate its role in different cellular processes and signaling pathways. Its availability as a recombinant protein allows for easy manipulation and characterization in these studies.

Conclusion

In summary, Recombinant Human LIN28A Protein is a highly conserved RNA-binding protein that plays a crucial role in regulating gene expression and development. Its unique structure and diverse activities make it a valuable tool in various fields of research, including stem cell biology and cancer research. As more studies continue to uncover its functions, Recombinant Human LIN28A Protein will likely find even more applications in the future.